In the Naka-ikemi Wetland, a cryptic species of the dojo loach, Misgurnus sp. Type I (called Type I loach herein), inhabits along with the common nominal species, Misgurnus anguillicaudatus (called Type II loach herein). In this study, the distribution patterns of two loaches were investigated by environmental DNA (eDNA) analysis and previously suggested habitat segregation between two sympatric loaches was discussed. Type I loach tended to have a limited distribution, mainly inhabiting the northern part of the wetland; Type II loach tended to be widely distributed across the central to southern parts of the wetland. Both loaches occurred sympatrically in some areas throughout the sampling periods, indicating that they did not completely segregate their habitats.
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We thank the NPO Naka-ikemi Net members for their assistance during the surveying at the Naka-ikemi Wetland. We also thank Dr. K. Hosoya, Kindai University, for providing valuable advice, and other members of our laboratory for assistance in the study. We would like to thank Editage (https://www.editage.jp) for English language editing. We express our gratitude to the three anonymous referees for providing valuable comments. This study was supported by JSPS KAKENHI (No. 17H03735 and 18J10088). The present study complied with current Japanese laws.
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Okada, R., Tsuji, S., Shibata, N. et al. Environmental DNA analysis provides an overview of distribution patterns of two dojo loach species within the Naka-ikemi Wetland, Fukui Prefecture, Japan. Ichthyol Res 68, 198–206 (2021). https://doi.org/10.1007/s10228-020-00776-0
- Cryptic species
- Type I loach
- Naka-ikemi Wetland
- Real-time PCR