We examined the genetic population structure of Lefua echigonia in the Ise Bay region based on the nucleotide sequences of the mitochondrial DNA cytochrome b region. Previous phylogeographic studies reported that only the Tokai lineage is distributed in this region; however, the present study revealed that the native Kinki lineage (Kuwana sub-lineage) is also distributed here. Moreover, the Tokai lineage was separated into three sub-lineages—Ise Bay, Mikawa-Shizuoka, and Nakatsugawa—with the Ise Bay and Mikawa-Shizuoka sub-lineages further subdivided into six and two local lineages, respectively. We revealed that L. echigonia has clear geographic differentiation within a single paleo-river basin.
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We express sincere thanks to Mr. Tomonari Asaka, Mr. Asaomi Asano, Mr. Kazuhisa Asano, Mr. Ryota Hamaguchi, Mr. Toshihiko Handa, Ms. Rina Ido, Dr. Takahiko Itai, Mr. Masaaki Kobayashi, Mr. Fumiya Komatsu, Ms. Chiaki Mineshita, Mr. Fumiyoshi Murase, Mr. Naofumi Nakanishi, Ms. Yukiko Shinkai, Mr. Go Shinkai, Ms. Miyu Suzuki, Ms. Rui Tajima, Dr. Taiga Yodo, and the Natural Environmental Division, Gifu City Government Office, for their assistance with obtaining the specimens, and to the Division of Genomics Research, Life Science Research Center, Gifu University, for their help with the DNA analysis. This work was supported by JSPS KAKENHI Grant Number 26250044 to author T. Mukai.
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Ito, G., Koya, Y., Kitanishi, S. et al. Genetic population structure of the eight-barbel loach Lefua echigonia in the Ise Bay region, a single paleo-river basin in central Honshu, Japan. Ichthyol Res 66, 411–416 (2019). https://doi.org/10.1007/s10228-019-00683-z
- Cytochrome b