Abstract
A recently discovered transplanted population of “Kunimasu” (Oncorhynchus kawamurae) in Lake Saiko, Yamanashi Prefecture, Japan, extinct in its original habitat, has been reported as almost never hybridizing with sympatric “Himemasu” (O. nerka). However, analyses of microsatellite and mitochondrial DNA markers disclosed extensive hybridization between these two species in Lake Motosu, Yamanashi Prefecture, a second lake to which Kunimasu had been transplanted. The rates of purebred Kunimasu, Kunimasu/Himemasu hybrids and purebred Himemasu were 0 %, 64.3 % and 35.7 %, respectively, and hybrid specimens had intermediate numbers of pyloric caeca and gill rakers compared with the parental species.
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References
Anderson EC, Thompson EA (2002) A model-based method for identifying species hybrids using multilocus genetic data. Genetics 160:1217–1229
Earl DA, vonHoldt BM (2012) STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv Genet Resour 4:359–361
Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software structure: a simulation study. Mol Ecol 14:2611–2620
Hubbs CL (1955) Hybridization between fish species in nature. Syst Zool 4:1–20
Hubisz MJ, Falush D, Stephens M, Pritchard JK (2009) Inferring weak population structure with the assistance of sample group information. Mol Ecol Resour 9:1322–1332
Jakobsson M, Rosenberg NA (2007) CLUMPP: a cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure. Bioinformatics 23:1801–1806
Jordan DS, Hubbs CL (1925) Record of fishes obtained by David Starr Jordan in Japan, 1922. Mem Carnegie Mus 10:93–346, pls 5–12
Moreira AL, Taylor EB (2015) The origin and genetic divergence of “black” kokanee, a novel reproductive ecotype of Oncorhynchus nerka. Can J Fish Aquat Sci 72:1584–1595
Muto N, Nakayama K, Nakabo T (2013) Distinct genetic isolation between “Kunimasu” (Oncorhynchus kawamurae) and “Himemasu” (O. nerka) in Lake Saiko, Yamanashi Prefecture, Japan, inferred from microsatellite analysis. Ichthyol Res 60:188–194
Nakabo T, Nakayama K, Muto N, Miyazawa M (2011) Oncorhynchus kawamurae ‘‘Kunimasu,’’ a deepwater trout, discovered in Lake Saiko, 70 years after extinction in the original habitat, Lake Tazawa, Japan. Ichthyol Res 58:180–183
Nakabo T, Tohkairin A, Muto N, Watanabe Y, Miura Y, Miura H, Aoyagi T, Kaji N, Nayakaya K, Kai Y (2014) Growth-related morphology of “Kunimasu” (Oncorhynchus kawamurae: family Salmonidae) from Lake Saiko, Yamanashi Prefecture, Japan. Ichthyol Res 61:115–130
Nakayama K, Muto N, Nakabo T (2013) Mitochondrial DNA sequence divergence between “Kunimasu” Oncorhynchus kawamurae and “Himemasu” O. nerka in Lake Saiko, Yamanashi Prefecture, Japan, and their identification using multiplex haplotype-specific PCR. Ichthyol Res 60:277–281
Olsen JB, Wilson SL, Kretschmer EJ, Jones KC, Seeb JE (2000) Characterization of 14 tetranucleotide microsatellite loci derived from sockeye salmon. Mol Ecol 9:2185–2187
Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959
Rosenberg NA (2004) Distruct: a program for the graphical display of population structure. Mol Ecol Notes 4:137–138
Srinivasa Rao KS, Lakshmi K (1999) Cryptic hybridization in marine fishes: significance of narrow hybrid zones in identifying stable hybrid populations. J Nat Hist 33:1237–1259
Sugiyama H (2000) Encyclopedia of Kunimasu. Akita Sakigake Shimpo, Akita
Terada S (1955) Fishes of Kai district. Yamanashi Ken Suisan Kenkyukai, Kofu
Tokui T (1964) Studies on the kokanee salmon (V). Transplantation of the kokanee salmon in Japan. Sci Rep Hokkaido Salmon Hatchery 18:73–90
Tokui T, Hikita T (1964) Hanamagari-seppari-masu in local name collected from Lake Motosu, Yamanashi Prefecture, Japan. Sci Rep Hokkaido Salmon Hatchery 18:117–119
Vähä JP, Primmer CR (2006) Efficiency of model-based Bayesian methods for detecting hybrid individuals under different hybridization scenarios and with different numbers of loci. Mol Ecol 15:63–72
Yamamoto S, Kitamura S, Sakano H, Morita K (2011) Genetic structure and diversity of Japanese kokanee Oncorhynchus nerka stocks as revealed by microsatellite and mitochondrial DNA markers. J Fish Biol 79:1340–1349
Acknowledgements
We are grateful to Motosuko Gyogyo Kyodo Kumiai (Lake Motosu Fisheries Cooperative), K. Watanabe, Y. Watanabe, Saiko Gyogyo Kyodo Kumiai (Lake Saiko Fisheries Cooperative), T. Aoyagi and M. Nozu for their help in collecting specimens; Yamanashi Fisheries Technology Center for information on stocking records of Himemasu; and N. Muto for helpful comments and discussion. We also thank Dr. G. S. Hardy for his critical reading of the manuscript.
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Nakayama, K., Tohkairin, A., Yoshikawa, A. et al. Detection and morphological characteristics of “Kunimasu” (Oncorhynchus kawamurae)/”Himemasu” (O. nerka) hybrids in Lake Motosu, Yamanashi Prefecture, Japan. Ichthyol Res 65, 270–275 (2018). https://doi.org/10.1007/s10228-018-0613-z
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DOI: https://doi.org/10.1007/s10228-018-0613-z