Ichthyological Research

, Volume 65, Issue 2, pp 270–275 | Cite as

Detection and morphological characteristics of “Kunimasu” (Oncorhynchus kawamurae)/”Himemasu” (O. nerka) hybrids in Lake Motosu, Yamanashi Prefecture, Japan

  • Kouji Nakayama
  • Akira Tohkairin
  • Akane Yoshikawa
  • Tetsuji Nakabo
Short Report


A recently discovered transplanted population of “Kunimasu” (Oncorhynchus kawamurae) in Lake Saiko, Yamanashi Prefecture, Japan, extinct in its original habitat, has been reported as almost never hybridizing with sympatric “Himemasu” (O. nerka). However, analyses of microsatellite and mitochondrial DNA markers disclosed extensive hybridization between these two species in Lake Motosu, Yamanashi Prefecture, a second lake to which Kunimasu had been transplanted. The rates of purebred Kunimasu, Kunimasu/Himemasu hybrids and purebred Himemasu were 0 %, 64.3 % and 35.7 %, respectively, and hybrid specimens had intermediate numbers of pyloric caeca and gill rakers compared with the parental species.


Oncorhynchus kawamurae Oncorhynchus nerka Hybridization Lake Motosu Microsatellite 



We are grateful to Motosuko Gyogyo Kyodo Kumiai (Lake Motosu Fisheries Cooperative), K. Watanabe, Y. Watanabe, Saiko Gyogyo Kyodo Kumiai (Lake Saiko Fisheries Cooperative), T. Aoyagi and M. Nozu for their help in collecting specimens; Yamanashi Fisheries Technology Center for information on stocking records of Himemasu; and N. Muto for helpful comments and discussion. We also thank Dr. G. S. Hardy for his critical reading of the manuscript.

Supplementary material

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Supplementary material 1 (XLS 60 kb)
10228_2018_613_MOESM2_ESM.tif (3.5 mb)
Supplementary material 2 (TIFF 3602 kb)


  1. Anderson EC, Thompson EA (2002) A model-based method for identifying species hybrids using multilocus genetic data. Genetics 160:1217–1229Google Scholar
  2. Earl DA, vonHoldt BM (2012) STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conserv Genet Resour 4:359–361CrossRefGoogle Scholar
  3. Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software structure: a simulation study. Mol Ecol 14:2611–2620CrossRefPubMedGoogle Scholar
  4. Hubbs CL (1955) Hybridization between fish species in nature. Syst Zool 4:1–20Google Scholar
  5. Hubisz MJ, Falush D, Stephens M, Pritchard JK (2009) Inferring weak population structure with the assistance of sample group information. Mol Ecol Resour 9:1322–1332Google Scholar
  6. Jakobsson M, Rosenberg NA (2007) CLUMPP: a cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure. Bioinformatics 23:1801–1806Google Scholar
  7. Jordan DS, Hubbs CL (1925) Record of fishes obtained by David Starr Jordan in Japan, 1922. Mem Carnegie Mus 10:93–346, pls 5–12Google Scholar
  8. Moreira AL, Taylor EB (2015) The origin and genetic divergence of “black” kokanee, a novel reproductive ecotype of Oncorhynchus nerka. Can J Fish Aquat Sci 72:1584–1595Google Scholar
  9. Muto N, Nakayama K, Nakabo T (2013) Distinct genetic isolation between “Kunimasu” (Oncorhynchus kawamurae) and “Himemasu” (O. nerka) in Lake Saiko, Yamanashi Prefecture, Japan, inferred from microsatellite analysis. Ichthyol Res 60:188–194Google Scholar
  10. Nakabo T, Nakayama K, Muto N, Miyazawa M (2011) Oncorhynchus kawamurae ‘‘Kunimasu,’’ a deepwater trout, discovered in Lake Saiko, 70 years after extinction in the original habitat, Lake Tazawa, Japan. Ichthyol Res 58:180–183Google Scholar
  11. Nakabo T, Tohkairin A, Muto N, Watanabe Y, Miura Y, Miura H, Aoyagi T, Kaji N, Nayakaya K, Kai Y (2014) Growth-related morphology of “Kunimasu” (Oncorhynchus kawamurae: family Salmonidae) from Lake Saiko, Yamanashi Prefecture, Japan. Ichthyol Res 61:115–130Google Scholar
  12. Nakayama K, Muto N, Nakabo T (2013) Mitochondrial DNA sequence divergence between “Kunimasu” Oncorhynchus kawamurae and “Himemasu” O. nerka in Lake Saiko, Yamanashi Prefecture, Japan, and their identification using multiplex haplotype-specific PCR. Ichthyol Res 60:277–281Google Scholar
  13. Olsen JB, Wilson SL, Kretschmer EJ, Jones KC, Seeb JE (2000) Characterization of 14 tetranucleotide microsatellite loci derived from sockeye salmon. Mol Ecol 9:2185–2187Google Scholar
  14. Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959Google Scholar
  15. Rosenberg NA (2004) Distruct: a program for the graphical display of population structure. Mol Ecol Notes 4:137–138Google Scholar
  16. Srinivasa Rao KS, Lakshmi K (1999) Cryptic hybridization in marine fishes: significance of narrow hybrid zones in identifying stable hybrid populations. J Nat Hist 33:1237–1259Google Scholar
  17. Sugiyama H (2000) Encyclopedia of Kunimasu. Akita Sakigake Shimpo, AkitaGoogle Scholar
  18. Terada S (1955) Fishes of Kai district. Yamanashi Ken Suisan Kenkyukai, KofuGoogle Scholar
  19. Tokui T (1964) Studies on the kokanee salmon (V). Transplantation of the kokanee salmon in Japan. Sci Rep Hokkaido Salmon Hatchery 18:73–90Google Scholar
  20. Tokui T, Hikita T (1964) Hanamagari-seppari-masu in local name collected from Lake Motosu, Yamanashi Prefecture, Japan. Sci Rep Hokkaido Salmon Hatchery 18:117–119Google Scholar
  21. Vähä JP, Primmer CR (2006) Efficiency of model-based Bayesian methods for detecting hybrid individuals under different hybridization scenarios and with different numbers of loci. Mol Ecol 15:63–72Google Scholar
  22. Yamamoto S, Kitamura S, Sakano H, Morita K (2011) Genetic structure and diversity of Japanese kokanee Oncorhynchus nerka stocks as revealed by microsatellite and mitochondrial DNA markers. J Fish Biol 79:1340–1349Google Scholar

Copyright information

© The Ichthyological Society of Japan 2018

Authors and Affiliations

  1. 1.Division of Applied Biosciences, Graduate School of AgricultureKyoto UniversityKyotoJapan
  2. 2.The Kyoto University Museum, Kyoto UniversityKyotoJapan
  3. 3.Kyoto Prefectural Agriculture, Forestry and Fisheries Technology CenterKyotoJapan
  4. 4.Japan Sea National Fishery Research InstituteNiigataJapan

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