Ichthyological Research

, Volume 63, Issue 1, pp 68–78 | Cite as

Cobitis sakahoko, a new species of spined loach (Cypriniformes: Cobitidae) from southern Kyushu Island, Japan

  • Jun Nakajima
  • Yuzuru Suzawa
Full Paper


We describe a new species of spined loach, Cobitis sakahoko, based on the holotype and nine paratypes collected from the Oyodo River system in the southern region of Kyushu Island, Japan. This species is distinguished from its congeners by the following combination of characteristics: lamina circularis at the base of the pectoral fin in adult males, a rectangular plate with a neck in the mid-lower part; the upper segments of the first branched soft ray of the pectoral fin broad; snout relatively short, length 32.1–38.2% of head length; long maxillary barbel, longer than the eye diameter; prepelvic myotome number 14; and line L5 organised in 8–13 oblong or ovoid blotches.


Cobitoidei Freshwater fish Cobitis sp. Oyodo form Oyodo-shima-dojyô 



We thank S. Toosaki, T. Iwasaki and M. Inamitsu for support in sample collection and Y. Kimizuka and M. Watanabe for their invaluable information. We are obliged to H. Senou (KPM) and Y. Sato (TKPM) for the donation of specimens, A. Tawa (Kyushu University) for taking the X-ray photographs, R. Uchiyama (Nature Photographer) for providing a valuable photograph, and the handling editor for giving helpful comments. This study was partly supported by Grants-in-Aid for JSPS Fellows (No. 08J02830).


  1. Akasaki M (1995) Miyazaki-ken no umi to kawa no gyokairui (9) Miyazaki-ken no kasen no sakana (5). Miyazaki no Shizen 11:78–88Google Scholar
  2. Buj I, Šanda R, Marčić Z, Ćaleta M, Mrakovčić M (2014) Combining morphology and genetics in resolving taxonomy – a systematic revision of spined loaches (genus Cobitis, Cypriniformes, Actinopterygii) in the Adriatic watershed. Plos One 9:e99833Google Scholar
  3. Chen Y, Chen Y (2013) Three new species of cobitid fish (Teleostei, Cobitidae) from the River Xinjiang and the River Le’anjiang, tributaries of Lake Poyang of China, with remarks on their classification. Folia Zool 62:83–95Google Scholar
  4. Chen YF, Chen YX (2005) Secondary sexual characters, pigmentation zones of Gambetta and taxonomical revision the genus Cobitis from China (Pisces, Cobitidae, Cobitinae). Acta Zootax Sinica 30:647–658Google Scholar
  5. Chen YX, Chen YF, He DK (2013) A new species of spined loach (Osteichthyes, Cobitidae) from the Pearl River, Guangxi of China. Acta Zootax Sinica 38:377–387Google Scholar
  6. Erkakan F, Özdemir F (2014) The first new cave fish species, Cobitis damlae (Teteostei: Cobitidae) from Turkey. Hacettepe J Biol Chem 42:275–279Google Scholar
  7. Hosoya K (2013) Cobitidae. In: Nakabo T (ed) Fishes of Japan with pictorial keys to the species third edition. Tokai Univ Press, Hadano, pp 328–334, 1819–1822Google Scholar
  8. Ikeda H (1936) On the sexual dimorphism and the taxonomical status of some Japanese loaches. Zool Mag Tokyo 48:983–994Google Scholar
  9. Jordan DS, Snyder JO (1901) List of fishes collected in 1883 and 1885 by Pierre Louis Jouy and preserved in the United States National Museum, with descriptions of six new species. Proc U S Nat Mus 28:739–769Google Scholar
  10. Kim IS (2009) A review of the spined loaches, family Cobitidae (Cypriniformes) in Korea. Korean J Ichthyol 21 (Suppl):7–28Google Scholar
  11. Kitagawa E, Hoshino K, Okazaki T, Kitagawa T (2004) Cobitis biwae from Oita River system in Oita Prefecture, Japan, and its biogeographic origin. Jpn J Ichthyol 51:117–122Google Scholar
  12. Kitagawa T, Jeon SR, Kitagawa E, Yoshioka M, Kashiwagi M, Okazaki T (2005) Genetic relationships among the Japanese and Korean striated spined loach complex (Cobitidae: Cobitis) and their phylogenetic positions. Ichthyol Res 52:111–122Google Scholar
  13. Kitagawa T, Watanabe M, Kitagawa E, Yoshioka M, Kashiwagi M, Okazaki T (2003a) Phylogeography and the maternal origin of the tetraploid form of the Japanese spined loach, Cobitis biwae, revealed by mitochondrial DNA analysis. Ichthyol Res 50:318–325Google Scholar
  14. Kitagawa T, Watanabe M, Kobayashi T, Yoshioka M, Kashiwagi M, Okazaki T (2001) Two genetically divergent groups in the Japanese spined loach, Cobitis takatsuensis, and their phylogenetic relationships among Japanese Cobitis inferred from mitochondrial DNA analysis. Zool Sci 18:249–259Google Scholar
  15. Kitagawa T, Yoshioka M, Kashiwagi M, Okazaki T (2003b) Genetic structure of a Japanese allotetraploid loach of the genus Cobitis (Osteichthyes, Cobitidae). Folia Biol (Kraków) 51:93–100Google Scholar
  16. Kottelat M (2012) Conspectus cobitidum: an inventory of the loaches of the world (Teleostei: Cypriniformes: Cobitidae). Raffles Bull Zool Suppl 26:1–199Google Scholar
  17. Kottelat M, Freyhof J (2007) Handbook of European freshwater fishes. Publ Kottelat, CornolGoogle Scholar
  18. Kyushu Regional Development Bureau (2006) River improvement plan, Oyodo River system. Kyushu Regional Development Bureau, MLIT, Government of Japan, FukuokaGoogle Scholar
  19. Linnaeus C (1758) Systema naturae per regna tria naturae, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis. Tomus I. Editio decima, reformata. Salvius, HolmiaeGoogle Scholar
  20. Minamori S (1951) Hybridization and classification in spinous loaches. Jpn J Ichthyol 1:215–225Google Scholar
  21. Ministry of the Environment (2015) Red data book 2014, brackish water and freshwater fish. Gyosei, TokyoGoogle Scholar
  22. Miyazaki Prefecture (2000) The red data species of plants and animals in Miyazaki Prefecture. Miyazaki Prefecture Environmental Science Association, MiyazakiGoogle Scholar
  23. Mizuno N (1970) A new species of cobitid fish from Japan. Mem Ehime Univ Sci Ser B (Biol) 6:1–11Google Scholar
  24. Mousavi-Sabet M, Vatandoust S, Esmaeili HR, Geiger MF, Freyhof HJ (2015) Cobitis avicennae, a new species of spined loach from the Tigris River drainage (Teleostei: Cobitidae). Zootaxa 3914:558–568Google Scholar
  25. Nakajima J (2012) Taxonomic study of the Cobitis striata complex (Cypriniformes, Cobitidae) in Japan. Zootaxa 3586:103–130Google Scholar
  26. Nakajima J (2015) Cobitis sp. Oyodo form. In: Ministry of the Environment (ed) Red data book 2014, brackish water and freshwater fish. Gyosei, Tokyo, pp 192–193Google Scholar
  27. Nakajima J, Nakamura T, Suzawa Y (2011) Record of peculiar Cobitis (Cypriniformes: Cobitidae) from the Oyodo River system in Miyazaki Prefecture, southern Kyushu Island, Japan. Jpn J Ichthyol 58:153–160Google Scholar
  28. Nakajima J, Suzawa Y, Shimizu T, Saitoh K (2012) Proposal of standard Japanese names for Japanese loaches in the genus Cobitis (Cobitidae). Jpn J Ichthyol 59:86–95Google Scholar
  29. Nalbant T (1963) A study of the genera of Botunae and Cobitinae. Trav Mus Nat d’Hist Nat “Grigore Antipa” 4:343–379Google Scholar
  30. Okada Y, Ikeda H (1939) A revision of the Japanese striped loaches, referred to the genus Cobitis. Sci Rep Tokyo Bunrika Daigaku Sec B 4:89–104Google Scholar
  31. Perdices A, Doadrio I (2001) The molecular systematics and biogeography of the European Cobitis based on mitochondrial DNA sequences. Mol Phyl Evol 19:468–478Google Scholar
  32. Perdices A, Vasil’ev V, Vasil’eva E (2012) Molecular phylogeny and intraspecific structure of loaches (genera Cobitis and Misgurnus) from the Far East region of Russia and some conclusions on their systematics. Ichthyol Res 59:113–123Google Scholar
  33. Saitoh K, Kobayashi T, Ueshima R, Numachi K (2000) Analysis of mitochondrial and satellite DNAs on spined loaches of the genus Cobitis from Japan have revealed relationships among populations of three diploid-tetraploid complexes. Folia Zool 49 (Suppl 1):9–16Google Scholar
  34. Sawada Y (1982) Phylogeny and zoogeography of the superfamily Cobitoidea (Cyprinoidei, Cypriniformes). Mem Fac Fish Hokkaido Univ 28:65–223Google Scholar
  35. Šlechtová V, Bohlen J, Perdices A (2008) Molecular phylogeny of the freshwater fish family Cobitidae (Cypriniformes: Teleostei): Delimitation of genera, mitochondrial introgression and evolution of sexual dimorphism. Mol Phyl Evol 47:812–831Google Scholar
  36. Suzawa Y (2006) A new loach, Cobitis shikokuensis (Teleostei: Cobitidae), from Shikoku Island, Japan. Ichthyol Res 53:315–322Google Scholar
  37. Takeda R, Fujie K (1945) Distribution of some color pattern types of Cobitis taenia. Zool Mag Tokyo 56:1–5Google Scholar
  38. Takeno M, Kashiwagi S, Kitagawa T (2010) A distinctive allotetraploid spined loach population (genus Cobitis) from Tango District, Kyoto Prefecture, Japan. Jpn J Ichthyol 572:105–112Google Scholar
  39. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729Google Scholar
  40. Tang Q, Liu H, Mayden R, Xiong B (2006) Comparison of evolutionary rates in the mitochondrial DNA cytochrome b gene and control region and their implications for phylogeny of the Cobitoidea (Teleostei: Cypriniformes). Mol Phyl Evol 39:347–357Google Scholar
  41. Watanabe K, Abe T, Iwata A (2009) Phylogenetic position and generic status of the Japanese botiid loach. Ichthyol Res 56:421–425Google Scholar

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© The Ichthyological Society of Japan 2015

Authors and Affiliations

  1. 1.Fukuoka Institute of Health and Environmental SciencesDazaifuJapan
  2. 2.Institute of River Biology, Inc.MunakataJapan

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