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Ichthyological Research

, Volume 61, Issue 3, pp 201–206 | Cite as

Cytogenetic study in Corydoras britskii (Siluriformes: Callichthyidae), using different chromosomal banding and fluorescence hybridization in situ with rDNA probes

  • Fábio Hiroshi Takagui
  • Natalia Bortolazzi Venturelli
  • Tatiane Ramos Sampaio
  • Ana Lúcia Dias
  • Lúcia Giuliano-Caetano
Full Paper

Abstract

Cytogenetic analyses were performed on Corydoras britskii from the Miranda River basin, an important river located in the Pantanal, Mato Grosso do Sul State, Brazil. The karyotype of this species comprises 90 chromosomes and a karyotype formula of 4m + 10 sm + 22 st + 54a. The nucleolus organizer regions were detected by impregnation with silver nitrate and FISH with an 18S rDNA probe on the short arm of three acrocentric chromosomes. The constitutive heterochromatin is distributed in pericentromeric and interstitial positions, and also associated with the NORs. The HinfI restriction endonuclease was used and showed homology with practically all types of heterochromatin observed in C. britskii, except for two interstitial heterochromatic blocks present in a subtelocentric pair. The fluorochrome staining evidenced six chromosomes with chromomycin-positive signals indicating that both the heterochromatin interspersed with NORs and some heterochromatic blocks were rich in GC base pairs. FISH using a 5S rDNA probe revealed the presence of these regions in only one subtelocentric pair in the interstitial position. The obtained data substantiate the karyotype diversity of the genus Corydoras and provide novel information about the composition of heterochromatin and location of 5S and 18S rDNA sites.

Keywords

Karyotype Heterochromatin Fluorochrome 18S rDNA 5S rDNA 

Notes

Acknowledgments

The authors are grateful to the State University of Londrina—CCB/Dept. General Biology for providing their laboratories; to CNPq and CAPES for their financial support; and to IBAMA for allowing the collection of biological material.

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Copyright information

© The Ichthyological Society of Japan 2014

Authors and Affiliations

  • Fábio Hiroshi Takagui
    • 1
  • Natalia Bortolazzi Venturelli
    • 1
  • Tatiane Ramos Sampaio
    • 1
  • Ana Lúcia Dias
    • 1
  • Lúcia Giuliano-Caetano
    • 1
  1. 1.Department of General Biology, CCBUniversidade Estadual de LondrinaLondrinaBrazil

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