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Ichthyological Research

, Volume 60, Issue 4, pp 390–395 | Cite as

Molecular identification and morphological description of totoaba Totoaba macdonaldi and curvina Cynoscion reticulatus preflexion larvae (Perciformes: Sciaenidae)

  • Noé Díaz-Viloria
  • Laura Sánchez-Velasco
  • Ricardo Perez-Enriquez
  • Sylvia P. A. Jiménez-Rosenberg
Short Report

Abstract

Preflexion larvae of Sciaenidae taxa collected in the upper Gulf of California on 29 March 2011, were identified as Totoaba macdonaldi (n = 4; notochord length 2.83 mm ± 0.22), an endemic species, and Cynoscion reticulatus (n = 11; notochord length 4.51 mm ± 0.47) on the basis of genetic divergence of partial sequences of 16S rRNA gene (448 base pairs) of mitochondrial DNA. Molecular identification was supported by clustering of preflexion larvae with adult specimens in the phylogenetic analysis, intraspecific (<1 %), and interspecific (>1 %) genetic divergences. Detailed observations allowed morphological descriptions and suggested diagnostic pigmentation in ventral midline of gut, one in T. macdonaldi, but three to four pairs in C. reticulatus; no spots were present on the dorsal tail in T. macdonaldi, but one or two were present in C. reticulatus. Totoaba macdonaldi had more than 20 pigment spots on the ventral tail, but there were only six in C. reticulatus. These larvae descriptions would be useful to identify areas and period of spawning of both species in ichthyoplankton studies.

Keywords

Preflexion fish larvae Mitochondrial DNA Totoaba macdonaldi Cynoscion reticulatus Upper Gulf of California 

Notes

Acknowledgments

We thank the fishery cooperative “Punta Estrella” at San Felipe, B.C. Juan P. Lazo Corvera of CICESE donated four and two specimens of T. macdonaldi and C. reticulatus, respectively. Philip A. Hastings and H. J. Walker from SIO donated C. othonopterus, L. pacificus, and M. megalops specimens (one each). Jose M. Grijalva Chon from USON donated five specimens of M. megalops. Adrían F. González Acosta and José De La Cruz Agüero of CICIMAR identified adult fish and Alejandra G. Sandoval Lugo and Anaís L. Lucero Olachea assisted with laboratory analyses. Editorial services were provided by Ira Fogel of CIBNOR. N.D.V. received funding from “Programa de Apoyos Complementarios para la Consolidación Institucional de Grupos de Investigación”. L.S.V. received financial support from the project “Asociaciones de larvas de peces, hábitat trófico y su relación con la estructura física en giros del Golfo de California” of CICIMAR, and a grant to Miguel F. Lavín of CICESE for “Ecological monitoring of the upper Gulf of California”. Preparation of the manuscript was partly funded by CIBNOR grant AC0.26 to R.P.E.

Supplementary material

10228_2013_359_MOESM1_ESM.doc (56 kb)
Supplementary material 1 (DOC 56 kb)

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Copyright information

© The Ichthyological Society of Japan 2013

Authors and Affiliations

  • Noé Díaz-Viloria
    • 1
  • Laura Sánchez-Velasco
    • 1
  • Ricardo Perez-Enriquez
    • 2
  • Sylvia P. A. Jiménez-Rosenberg
    • 1
  1. 1.Instituto Politécnico Nacional-Centro Interdisciplinario de Ciencias Marinas (IPN-CICIMAR)La PazMexico
  2. 2.Centro de Investigaciones Biológicas del Noroeste (CIBNOR)Instituto Politécnico Nacional 195La PazMexico

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