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Distinct genetic isolation between “Kunimasu” (Oncorhynchus kawamurae) and “Himemasu” (O. nerka) in Lake Saiko, Yamanashi Prefecture, Japan, inferred from microsatellite analysis

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Abstract

The possibility of interspecific hybridization between Kunimasu (Oncorhynchus kawamurae) and Himemasu (Oncorhynchus nerka) was investigated in a large number of specimens, in a search for basic data relevant to conservation needs. A Bayesian-based clustering method using five microsatellite DNA loci separated 144 specimens from Lake Saiko, Yamanashi Prefecture, into two genetically distinct groups, corresponding to Kunimasu and Himemasu. Application of a threshold of individual proportion of membership of 0.90, so as to separate hybrids from purebreds, resulted in all specimens except two having high probability of purebred. These results implied that Kunimasu and Himemasu have been maintained as independent genetic entities in Lake Saiko.

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Acknowledgments

We thank Yasuaki Miura, Y. Watanabe, Hisashi Miura, Hiroshi Miura, Manabu Miura, K. Mitsui, K. Takahashi, T. Aoyagi, N. Kaji, M. Ohashi, S. Sasaki, M. Nozu, and N. Sakai for their help in collecting specimens, and A. Tokairin and K. Kimura for their assistance in the laboratory. We also thank G. S. Hardy for his critical reading of the manuscript. This study was partly supported by the Core Stage Backup Research Fund of Kyoto University and a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (24370036).

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Authors and Affiliations

  1. Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan

    Nozomu Muto

  2. The Kyoto University Museum, Kyoto University, Yoshida, Sakyo, Kyoto, 606-8501, Japan

    Nozomu Muto & Tetsuji Nakabo

  3. Field Science Education and Research Center, Kyoto University, Kitashirakawa-Oiwake, Sakyo, Kyoto, 606-8502, Japan

    Kouji Nakayama

Authors
  1. Nozomu Muto
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  2. Kouji Nakayama
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  3. Tetsuji Nakabo
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Correspondence to Kouji Nakayama.

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Muto, N., Nakayama, K. & Nakabo, T. Distinct genetic isolation between “Kunimasu” (Oncorhynchus kawamurae) and “Himemasu” (O. nerka) in Lake Saiko, Yamanashi Prefecture, Japan, inferred from microsatellite analysis. Ichthyol Res 60, 188–194 (2013). https://doi.org/10.1007/s10228-012-0323-x

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  • DOI: https://doi.org/10.1007/s10228-012-0323-x

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