Abstract
The genetic relationships of a population of brook lamprey Lampetra planeri from Spain were analysed using a fragment of the mitochondrial non-coding region in order to describe its relationships with other European Lampetra populations. DNA sequences were obtained from ten larvae, all corresponding to a single private haplotype. Its closest haplotype differed by five mutations and was found in several samples of Lampetra fluviatlis, both from the North Sea and the Baltic. These results argue for the great conservation value of this brook lamprey population, likely originated from L. fluviatilis ancestors.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Almada VC, Pereira AM, Robalo JI, Fonseca JP, Levy A, Maia C, Valente A (2008) Mitochondrial DNA fails to reveal genetic structure in sea-lampreys along European shores. Mol Phylogenet Evol 46:391–396
Alvarez J, Doadrio I (1986) Sobre la presencia de Lampetra planeri Bloch, 1784 en España. Acta Verteb 13:158–160
Clement M, Posada D, Crandall KA (2000) TCS: a computer program to estimate gene genealogies. Mol Ecol 9:1657–1659
Espanhol R, Almeida PR, Alves J (2007) Evolutionary history of lamprey paired species Lampetra fluviatilis (L.) and Lampetra planeri (Bloch) as inferred from mitochondrial DNA variation. Mol Ecol 16:1909–1924
Excoffier LGL, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinf 1:47–50
Hardisty, MW (2006) Lampreys. Life without jaws. Forrest text, Ceredigion, UK
Kottelat M, Freyhof J (2007) Handbook of European freshwater fishes. Kottelat, Cornol
Lee WJ, Kocher D (1995) Complete sequence of a sea lamprey (Petromyzon marinus) mitochondrial genome: early establishment of a vertebrate genome organization. Genetics 139:873–887
Pereira AM, Robalo JI, Freyhof J, Maia C, Fonseca JP, Valente A, Almada VC (2010) Phylogeographical analysis reveals multiple conservation units in brook lampreys of Portuguese streams. J Fish Biol 77:361–371
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York
Takezaki N, Figueroa F, Zaleska-Rutczynska Z, Klein J (2003) Molecular phylogeny of early vertebrates: monophyly of the agnathans as revealed by sequences of 35 genes. Mol Biol Evol 20:287–292
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882
Zanandrea G (1959) Speciation among lampreys. Nature 184:280
Acknowledgments
We thank C. Sousa-Santos for her help. This work was supported by a grant from Iceland, Liechtenstein and Norway through the EEA Financial Mechanism and the Norwegian Financial Mechanism, by the project MarinERA “Marine phylogeographic structuring during climate change: the signature of leading and rear edge of range shifting populations” and from the Eco-Ethology Research Unit (331/94) Pluriannual financing program (Fundação para a Ciência e a Tecnologia). Collection of specimens complied with the current laws of each country.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
About this article
Cite this article
Pereira, A.M., Almada, V.C. & Doadrio, I. Genetic relationships of brook lamprey of the genus Lampetra in a Pyrenean stream in Spain. Ichthyol Res 58, 278–282 (2011). https://doi.org/10.1007/s10228-011-0218-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10228-011-0218-2