Abstract
Cytogenetic analysis in three Rineloricaria pentamaculata populations revealed diploid number 2n = 56 chromosomes, karyotype formula 8m/sm + 48st/a and FN = 64. Owing to the presence of the heteromorphic chromosome pair with a big submetacentric chromosome and a small acrocentric one in both males and females, 42.9% of specimens in the Tauá Stream population had the karyotype formula 9m/sm + 47st/a and FN = 65. Analysis of the nucleolus-organizing region by Ag-NOR and FISH techniques showed a single NOR system at pair 5 for R. pentamaculata populations of the Keller River and the Tauá Stream. However, specimens of populations of the Tatupeba Stream had multiple NOR systems at pairs 5 and 8. A constitutive heterochromatin pattern in R. pentamaculata is mainly distributed in the pericentromeric and telomeric regions with interstitial markers in certain chromosomes. Heterochromatin is located in the telomeric and centromeric positions of the acrocentric chromosome in the heteromorphic pair of the Tauá Stream population. In the submetacentric chromosome the markings are located in the telomeric (short arm), pericentomeric and interstitial (long arm) positions. The origins of polymorphisms are discussed.
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Acknowledgments
We thank the Brazilian agency Capes for financial support and Cláudio Henrique Zawadzki for aid in the collecting. We declare that any experiments comply with the current laws of the country in which they were performed.
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Porto, F.E., Portela-Castro, A.L.B. & Martins-Santos, I.C. Chromosome polymorphism in Rineloricaria pentamaculata (Loricariidae, Siluriformes) of the Paraná River basin. Ichthyol Res 58, 225–231 (2011). https://doi.org/10.1007/s10228-011-0215-5
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DOI: https://doi.org/10.1007/s10228-011-0215-5