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Monitoring of Na+/K+-ATPase mRNA expression in the cinnamon clownfish, Amphiprion melanopus, exposed to an osmotic stress environment: profiles on the effects of exogenous hormone

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Abstract

We examined changes in the expression of Na+/K+-ATPase mRNA in the gills of the cinnamon clownfish using quantitative real-time PCR in an osmotically changing environment [seawater (35 psu; practical salinity unit, 1 psu ≈ 1‰) → brackish water (17.5 psu) and brackish water with prolactin]. The expression of Na+/K+-ATPase mRNA in gills was increased after the transfer to brackish water, and the expression was repressed by prolactin treatment. Also, activities of gill Na+/K+-ATPase and plasma cortisol levels increased after the transfer to brackish water and were repressed in brackish water with prolactin treatment. Na+/K+-ATPase-immunoreactive cells were almost consistently observed in the gill filaments, but absent from the lamella epithelia. The plasma osmolality level decreased in brackish water, but the level of this parameter increased in brackish water with prolactin treatment during salinity change. These results suggest that the Na+/K+-ATPase gene plays an important role in osmoregulation in gills, and prolactin improves the hyperosmoregulatory ability of cinnamon clownfish in a brackish water (hypoosmotic) environment.

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Acknowledgments

This research was supported by the Technology Development Program for Fisheries, Ministry for Food, Agriculture, Forestry and Fisheries, Korea, and by a grant from the National Fisheries Research and Development Institute (RP-2011-AQ-010). All animal experiments were approved by the Institutional Animal Ethics Committee and followed National Research Council Guidelines.

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Authors and Affiliations

  1. East Sea Fisheries Research Institute, National Fisheries Research and Development Institute, Gangneung, 210-861, Republic of Korea

    Mi Seon Park

  2. Division of Marine Environment and BioScience, Korea Maritime University, Busan, 606-791, Republic of Korea

    Hyun Suk Shin & Cheol Young Choi

  3. Division of Electrical and Electronic Engineering, Korea Maritime University, Busan, 606-791, Republic of Korea

    Gyung-Suk Kil

  4. Department of Marine Life Sciences, Jeju National University, Jeju, Jeju Special Self-Governing Province, 690-756, Republic of Korea

    Jehee Lee

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  1. Mi Seon Park
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  2. Hyun Suk Shin
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  5. Cheol Young Choi
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Correspondence to Cheol Young Choi.

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Park, M.S., Shin, H.S., Kil, GS. et al. Monitoring of Na+/K+-ATPase mRNA expression in the cinnamon clownfish, Amphiprion melanopus, exposed to an osmotic stress environment: profiles on the effects of exogenous hormone. Ichthyol Res 58, 195–201 (2011). https://doi.org/10.1007/s10228-011-0207-5

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  • DOI: https://doi.org/10.1007/s10228-011-0207-5

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