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Genetic identification of fish eggs collected in Sendai Bay and off Johban, Japan

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Abstract

Mitochondrial DNA typing of 52 fish eggs collected in Sendai Bay and off Johban revealed ten haplotypes of the 225–251 bp region of 16S ribosomal DNA. Comparing with known sequences of fishes, we identified them as Trachurus japonicus (Carangidae), Pagrus major, Evynnis japonica (Sparidae), Paralichthys olivaceus, Pseudorhombus sp. (Paralichthidae), Pennahia argentata (Sciaenidae) and Platycephalus sp. (Platycephalidae). The occurrence of Trachurus japonicus and sparid eggs in the study area indicates significant extensions to the ranges of their spawning sites northward. Our method is useful for identifying fish eggs that are difficult to diagnose by morphology, and to uncover cryptic fish egg assemblages in the field.

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Acknowledgments

Nobuhiko Taniguchi and Masamichi Nakajima provided one of us (TT) with the opportunity to perform this collaborative work. We are grateful to crew of the research vessel Wakatakamaru, Sayuri Dasai, Ryo Nakano, Masakane Ohshima, Mitsuhiro Shoji and Michio Yoneda, for their help in the field survey. Special thanks go out to Fumie Abe and Shizuka Sasaki for their assistance in laboratory procedures. Masato Aritaki and Yuichiro Fujinami provided us with flatfish eggs to use as reference samples. This paper is a scientific contribution from the Fisheries Research Agency, Japan (FRA-TNFRI-B108).

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Author notes

  1. Kenji Saitoh

    Present address: National Research Institute of Fisheries Science, Fisheries Research Agency, 2-12-4 Fukuura, Kanazawa, Yokohama, Kanagawa, 236-8648, Japan

  2. Taro Tega

    Present address: IDEA Consultants Inc., Nagoya Office, 1-7-15 Irifune, Minato, Nagoya, Aichi, 455-0032, Japan

Authors and Affiliations

  1. Tohoku National Fisheries Research Institute, Fisheries Research Agency, 3-27-5 Shinhama, Shiogama, Miyagi, 985-0001, Japan

    Kenji Saitoh & Shinji Uehara

  2. Laboratory of Population Genetic Informatics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiya, Aoba, Sendai, Miyagi, 981-8555, Japan

    Taro Tega

Authors
  1. Kenji Saitoh
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  2. Shinji Uehara
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  3. Taro Tega
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Correspondence to Kenji Saitoh.

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Saitoh, K., Uehara, S. & Tega, T. Genetic identification of fish eggs collected in Sendai Bay and off Johban, Japan. Ichthyol Res 56, 200–203 (2009). https://doi.org/10.1007/s10228-008-0073-y

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  • DOI: https://doi.org/10.1007/s10228-008-0073-y

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