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Paternity testing of wild black rockfish Sebastes inermis (brownish type) from the Seto Inland Sea of Japan

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Abstract

We developed four microsatellite DNA loci to test for multiple paternity of black rockfish, Sebastes inermis, from the Seto Inland Sea of Japan. All loci showed a high degree of polymorphism (number of alleles per locus = 10–14, expected heterozygosity = 0.80) and discriminating power (probability of identity index = 3.71 × 10−6, exclusion probability = 0.999) in unrelated wild specimens (n = 32). Genotypic assignment of five dams (109–220 mm in total length) and 50 embryos from each dam (n = 50) indicated that four dams were mated with a single sire. Only for one dam and three of her embryos we could not exclude multiple paternity.

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Acknowledgments

We express special thanks to Mr. George Kandathil for constructive comments that have improved the manuscript. The present study was partly supported by a grant from the Japan Ministry of Education, Culture, Sports, Science and Technology.

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Authors and Affiliations

  1. Graduate School of Biosphere Sciences, Hiroshima University, Higashi-Hiroshima, Hiroshima, 739-8528, Japan

    Enrique Blanco Gonzalez, Yuki Teshima & Tetsuya Umino

  2. Hiroshima Prefectural Technology Research Institute Fisheries and Ocean Technology Center, Kure, Hiroshima, 737-1207, Japan

    Tomoya Murakami

  3. Hiroshima Prefectural Fisheries Coordination Office, Hiroshima, Hiroshima, 730-8511, Japan

    Kouji Yoshioka

  4. Marine Seed Releasing Technology Center, 591 Jeongdori, Wando, 537-806, South Korea

    Dal-Sang Jeong

Authors
  1. Enrique Blanco Gonzalez
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  2. Tomoya Murakami
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  3. Yuki Teshima
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  4. Kouji Yoshioka
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  5. Dal-Sang Jeong
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  6. Tetsuya Umino
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Corresponding author

Correspondence to Tetsuya Umino.

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Blanco Gonzalez, E., Murakami, T., Teshima, Y. et al. Paternity testing of wild black rockfish Sebastes inermis (brownish type) from the Seto Inland Sea of Japan. Ichthyol Res 56, 87–91 (2009). https://doi.org/10.1007/s10228-008-0055-0

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  • DOI: https://doi.org/10.1007/s10228-008-0055-0

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