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Genetic differences among three species of the genus Trichiurus (Perciformes: Trichiuridae) based on mitochondrial DNA analysis

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The mitochondrial DNA segment encoding the 16S ribosomal RNA (16S rRNA) gene sequence (ca. 600 bp) was compared among Trichiurus sp. 2 (sensu Nakabo, 2002) (obtained from various areas of Japan), T. japonicus Temminck and Schlegel (collected from various localities within Japan), and true T. lepturus Linnaeus (caught off the Atlantic coast of the United States and Brazil) of the family Trichiuridae using 10, 10, and 15 specimens, respectively. Based on phylogenetic analysis using a neighbor-joining (NJ) algorithm, the haplotypes of Trichiurus sp. 2, T. japonicus, and T. lepturus indicated three distinct monophyletic lineages, being supported by 100% bootstrap values with no haplotypes overlapping or sharing among the lineages. Trichiurus sp. 2, T. japonicus, and T. lepturus are genetically different from each other, suggesting that they are three distinct species.

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  • P Bleeker (1854) ArticleTitleNieuwe nalezingen op de ichthyologie van Japan Verh Bat Gen 37 1–132

    Google Scholar 

  • M Boeseman (1947) ArticleTitleRevision of the fishes collected by Burger and von Siebold in Japan Zool Meded 28 1–242

    Google Scholar 

  • Burhanuddin AI (2003) Review of the family Trichiuridae without a forked caudal fin (Perciformes). Ph D thesis, Kagoshima University, Kagoshima

  • Day F (1865) The fishes of Malabar. Fishes Malabar, London, pp i–xxxii + 1–293, pls 1–20

  • J Felsenstein (1985) ArticleTitleConfidence limits on phylogenies: an approach using the bootstrap Evolution 39 783–791 Occurrence Handle10.2307/2408678

    Article  Google Scholar 

  • HW Fowler (1936) A synopsis of the fishes of China Hong Kong Nature Hong Kong

    Google Scholar 

  • TA Hall (1999) ArticleTitleBioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT Nucleic Acids Symp Ser 41 95–98 Occurrence Handle1:CAS:528:DC%2BD3cXhtVyjs7Y%3D

    CAS  Google Scholar 

  • DS Jordan S Tanaka JO Snyder (1913) ArticleTitleA catalogue of the fishes of Japan J Coll Sci Imp Univ Tokyo 33 1–497

    Google Scholar 

  • N Karaiskuo AP Apostolidis A Triantafyllidis A Kouvatsi C Triantaphyllidis (2003) ArticleTitleGenetic identification and phylogeny of three species of the genus Trachurus based on mitochondrial DNA analysis Mar Biotechnol 5 493–504 Occurrence Handle10.1007/s10126-002-0099-5

    Article  Google Scholar 

  • M Kimura (1980) ArticleTitleA simple method for estimating evolutionary rates of base substitutions through comparative studies of DNA sequences J Mol Evol 16 111–120 Occurrence Handle7463489 Occurrence Handle1:CAS:528:DyaL3MXmtFSktg%3D%3D Occurrence Handle10.1007/BF01731581

    Article  PubMed  CAS  Google Scholar 

  • S Kimura K Matsuura (Eds) (2003) Fishes of Bitung, northern tip of Sulawesi, Indonesia Ocean Research Institute, The University of Tokyo Tokyo

    Google Scholar 

  • S Kumar K Tamura IB Jakobsen M Nei (2001) ArticleTitleMEGA 2: Molecular Evolutionary Genetics Analysis software Bioinformatics 17 1244–1245 Occurrence Handle11751241 Occurrence Handle1:CAS:528:DC%2BD38XmtVCktQ%3D%3D Occurrence Handle10.1093/bioinformatics/17.12.1244

    Article  PubMed  CAS  Google Scholar 

  • SC Lee KH Chang WL Wu HC Yang (1977) ArticleTitleFormosan ribbonfishes (Perciformes: Trichiuridae) Bull Inst Zool Acad Sin 16 77–84

    Google Scholar 

  • CS Li (1992) ArticleTitleHairtail fishes from Chinese coastal waters (Trichiuridae) Mar Sci Acad Sin 4 212–218 Occurrence Handle1:STN:280:By2B3sbmsFQ%3D

    CAS  Google Scholar 

  • SY Lin (1936) ArticleTitleNotes on hairtails and eels of China Bull Chekian Prov Fish Exp Sta 2 1–16

    Google Scholar 

  • K Mabuchi N Okuda T Kokita M Nishida (2003) ArticleTitleGenetic comparison of two color-morphs of Apogon properuptus from southern Japan Ichthyol Res 50 293–296 Occurrence Handle10.1007/s10228-003-0171-9

    Article  Google Scholar 

  • K Matsubara (1955) Fish morphology and hierarchy. Part 1 Ishizaki Shoten Tokyo

    Google Scholar 

  • M Miya M Nishida (1996) ArticleTitleMolecular phylogenetic perspective on the evolution of the deep-sea fish genus Cyclothone (Stomiiformes: Gonostomatidae) Ichthyol Res 43 375–398 Occurrence Handle10.1007/BF02347637

    Article  Google Scholar 

  • T Nakabo (1993) Family Trichiuridae T Nakabo (Eds) Fishes of Japan with pictorial keys to the species (in Japanese) Tokai University Press Tokyo 1142

    Google Scholar 

  • T Nakabo (2002) Family Trichiuridae T Nakabo (Eds) Fishes of Japan with pictorial keys to the species Tokai University Press Tokyo 1345

    Google Scholar 

  • Nakamura I, Parin NV (1993) FAO species catalogue, vol 15. Snake mackerels and cutlassfishes of the world (families Gempylidae and Trichiuridae). An annotated and illustrated catalogue of the snake mackerels, snoeks, escolars, gemfishes, sackfishes, domine, oilfish, cutlassfishes, scabbardfishes, hairtails and frostfishes known to date. FAO, Rome

  • N Saitou M Nei (1987) ArticleTitleThe neighbor-joining method: a new method for reconstructing phylogenetic trees Mol Biol Evol 4 406–425 Occurrence Handle3447015 Occurrence Handle1:STN:280:BieC1cbgtVY%3D

    PubMed  CAS  Google Scholar 

  • JD Thompson DG Higgins TJ Gibson (1994) ArticleTitleCLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice Nucleic Acids Res 22 4673–4680 Occurrence Handle7984417 Occurrence Handle1:CAS:528:DyaK2MXitlSgu74%3D

    PubMed  CAS  Google Scholar 

  • M Tokimura U Yamada T Irie (1995) ArticleTitleComments on taxonomy and distributions of the species of Trichiurus in the East China and Yellow Seas and adjacent waters (in Japanese) Seikaiku Suisan Kenkyusho News 80 12–14

    Google Scholar 

  • DW Tucker (1956) ArticleTitleStudies on trichiuroid fishes. 3. A preliminary revision of the family Trichiuridae Bull Br Mus Nat Hist Zool 4 73–130

    Google Scholar 

  • Yamada U, Shirai S, Irie T, Tokimura M, Deng S, Zheng Y, Li CS, Kim YU, Kim YS (1995) Names and illustrations of fishes from the East China Sea and the Yellow Sea (in Japanese, Chinese, and Korean). Overseas Fishery Cooperation Foundation, Tokyo, pp xi + 288

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Correspondence to Anirban Chakraborty.

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Chakraborty, A., Aranishi, F. & Iwatsuki, Y. Genetic differences among three species of the genus Trichiurus (Perciformes: Trichiuridae) based on mitochondrial DNA analysis. Ichthyol Res 53, 93–96 (2006).

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