Abstract
Vasotocin (VT) has been associated with the regulation of different aspects of social behavior (e.g., mating and aggression). Given the fact that androgens are also known to regulate reproductive behavior, we hypothesized that VT and androgens could be interacting, rather than acting independently, in the regulation of reproductive behavior. In the present study, we aimed to understand the effect of VT and its interaction with gonadal hormones (putatively androgens) on different aspects of reproductive behavior of a polygynous and territorial cichlid fish, the Mozambique tilapia (Oreochromis mossambicus). Using a within-subject design, we treated territorial males, that were previously castrated or sham-operated, with different dosages of VT as well as with a V1A receptor antagonist (Manning compound) and subsequently analyzed their behavior towards females and towards an intruder male. Our results showed that VT affected the behavior of territorial males towards females but not towards males. Specifically, VT-treated males interacted less with females (i.e., spent less time touching the transparent partition that allowed visual contact with females) and were less aggressive towards females than saline-treated males. Moreover, in sham-operated males, blocking V1A receptors increased the frequency of bites towards females in comparison to saline-treated males, but not in castrated males. This result suggests that VT down-regulates aggressiveness towards females through the action of V1A receptors in the gonads (putatively decreasing androgen secretion), and that androgens up-regulate this behavior. In summary, our results suggest that VT may modulate social behavior, through an interaction with gonadal hormones.
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This study was funded by a Fundação para a Ciência e a Tecnologia (FCT, Portugal) grant (EXCL/BIA-ANM/0549/2012) given to RO. OA and AF were supported by FCT Ph.D. fellowships (SFRH/BD/37187/2007 and SFRH/BD/102892/2014, respectively).
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OA and RO designed the experiments. OA performed behavioral experiments. AF analyzed the data. AF and RO wrote the paper, which was based on the Ph.D. thesis (chapter 3) of AF.
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Animal experimentation procedures were conducted in accordance with the European Communities Council Directive of 24 November 1986(86/609/EEC) and were approved by the Portuguese Veterinary Authority (Direcção Geral de Alimentação e Veterinária, Portugal; permit # 0421/000/000/2013).
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Almeida, O., Félix, A.S. & Oliveira, R.F. Interaction between vasotocin and gonadal hormones in the regulation of reproductive behavior in a cichlid fish. acta ethol 26, 31–43 (2023). https://doi.org/10.1007/s10211-022-00404-2
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DOI: https://doi.org/10.1007/s10211-022-00404-2