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Response of microbial community structure to natural and accelerated hydrarch successions in the boreal wetlands in northern Hokkaido, Japan

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Abstract

We estimated the effect of invading Sasa vegetation and accelerated terrestrialization on the microbial community structure in Sarobetsu-genya wetland (SGW) and Nakanominedaira wetland (NW) (original vegetation, Sphagnum). All examined peat-pore water samples were acidic. Electrical conductivity significantly differed between SGW and NW. Nonmetric multidimensional scaling (NMDS) and analysis of similarity based on denaturing gradient gel electrophoresis (DGGE) band patterns revealed differences in the bacterial community structure between the Sasa and Sphagnum vegetations at a depth of 10 cm in NW. In contrast, the bacterial NMDS profiles at all depths differed between the 2 wetlands rather than between the 2 vegetations. The archaeal community structure significantly differed between the wetlands at depths of 30 and 50 cm. The bacterial diversity index derived from the DGGE profiles significantly differed between the wetlands at all depths. The archaeal diversity index significantly differed between the wetlands at a depth of 50 cm. Sasa invasion affected the microbial community structure in the rhizosphere, up to a depth of 10 cm; this effect differed with the terrestrialization speed. These results suggest that in peat bogs subjected to artificially accelerated terrestrialization, the microbial community changes before the occurrence of the natural hydrarch ecological succession involving ground vegetation.

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Acknowledgments

We are grateful to Mutsumi Nomura, Kentaro Takagi, and Hajime Hojo of the Teshio Experimental Forest of Hokkaido University for their cooperation during fieldwork and for providing useful information regarding the study sites. This work was supported by the Ministry of Economy, Trade and Industry (METI) of Japan.

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Authors and Affiliations

  1. Horonobe Research Institute for the Subsurface Environment, Northern Advancement Center for Science and Technology, 5-3 Sakaemachi, Horonobe-cho, Hokkaido, 098-3221, Japan

    Masaru Akiyama, Satoru Shimizu & Yoji Ishijima

  2. Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, 739-8528, Japan

    Takeshi Naganuma

  3. Geoscience Research Laboratory Co., Ltd., 1794 Kamiwada, Yamato, Kanagawa, 242-0014, Japan

    Masaru Akiyama

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  1. Masaru Akiyama
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  2. Satoru Shimizu
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  3. Yoji Ishijima
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  4. Takeshi Naganuma
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Correspondence to Masaru Akiyama.

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Akiyama, M., Shimizu, S., Ishijima, Y. et al. Response of microbial community structure to natural and accelerated hydrarch successions in the boreal wetlands in northern Hokkaido, Japan. Limnology 11, 273–279 (2010). https://doi.org/10.1007/s10201-010-0311-7

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