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Thymic stromal lymphopoietin secretion of synovial fibroblasts is positively and negatively regulated by Toll-like receptors/nuclear factor-κB pathway and interferon-γ/dexamethasone

  • ORIGINAL ARTICLE
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Modern Rheumatology

Abstract

Thymic stromal lymphopoietin (TSLP) is an interleukin (IL)-7-like cytokine produced by epithelial cells and triggers dendritic cell-mediated Th2 type allergic inflammatory responses. This study investigated whether Toll-like receptor (TLR) ligands, lipopolysaccharide (LPS) and poly-IC affect TSLP production in synovial fibroblasts. Enzyme-linked immunosorbent assay showed that LPS and poly-IC upregulated TSLP production in synovial fibroblasts obtained from patients with rheumatoid arthritis (RA) and osteoarthritis (OA). In addition, we found that nuclear factor (NF)-κB inhibitor IMD-0354, dexamethasone, and interferon (IFN)-γ inhibited the LPS- and poly-IC-induced TSLP production in RA and OA synovial fibroblasts. Thus, LPS and poly-IC can upregulate TSLP via a NF-κB pathway in synovial fibroblasts, which is downregulated by dexamethasone and interferon (IFN)-γ. The current findings suggest that TSLP may be involved in the pathophysiology of inflammatory arthritis as well as allergic disease.

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Authors and Affiliations

  1. Department of Immunology, Faculty of Medicine, University of Yamanashi, Chuo, Japan

    Tetsuro Ozawa, Kensuke Koyama, Takashi Ando, Yuko Ohnuma, Kyosuke Hatsushika, Tetsuro Ohba & Atsuhito Nakao

  2. Department of Orthopaedic Surgery, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, 409-3898, Japan

    Kensuke Koyama, Tetsuro Ohba, Hajime Sugiyama & Yoshiki Hamada

  3. Atopy Research Center, Juntendo University School of Medicine, Tokyo, Japan

    Hideoki Ogawa, Ko Okumura & Atsuhito Nakao

Authors
  1. Tetsuro Ozawa
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  2. Kensuke Koyama
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  3. Takashi Ando
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  4. Yuko Ohnuma
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  5. Kyosuke Hatsushika
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  11. Atsuhito Nakao
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Correspondence to Kensuke Koyama.

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Ozawa, T., Koyama, K., Ando, T. et al. Thymic stromal lymphopoietin secretion of synovial fibroblasts is positively and negatively regulated by Toll-like receptors/nuclear factor-κB pathway and interferon-γ/dexamethasone. Mod Rheumatol 17, 459–463 (2007). https://doi.org/10.1007/s10165-007-0620-9

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  • DOI: https://doi.org/10.1007/s10165-007-0620-9

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