An optimum condition of MICP indigenous bacteria with contaminated wastes of heavy metal

Abstract

Heavy metals are naturally occurring trace elements present in the environment, including soil, water, and air. However, high heavy metal concentration contamination from waste is a serious environmental problem brought on by industrial activities. The research in this study evaluated different biomineralized CaCO3 forms from individual bacteria species in heavy-metal-contaminated soils and mine tailings. Scanning electron microscope (SEM) images of the crystals were used to characterize the precipitated CaCO3. Generally, urea-hydrolysis bacteria form minerals via a microbiologically induced calcite precipitation (MICP) process. These bacteria produce the urease enzyme, which leads to urea-hydrolysis. These bacteria were isolated from heavy-metal-contaminated soils and characterized for their potential utilization in the S/S process. Optimum conditions for indigenous bacterial growth were 30 °C and a pH range of 7–8; and growth patterns were further affected by the growth medium salinity. SEM and X-ray diffraction (XRD) analyses demonstrated that bioaccumulated heavy metal ions were deposited around the cell envelope as rhombohedral and sphere shaped crystalline carbonate minerals in optimum conditions. In this study, the authors hypothesize that the indigenous bacteria can effectively precipitate heavy metals in soil and mine tailing with the urea-hydrolysis enzyme, and play an important role in heavy metal stabilization.

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Acknowledgements

This study was supported by the Korea Ministry of Environment (MOE) as part of the GAIA Project (Geo-Advanced Innovative Action Project; No. 2015000550007).

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Correspondence to Jai-Young Lee.

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Kim, JH., Lee, JY. An optimum condition of MICP indigenous bacteria with contaminated wastes of heavy metal. J Mater Cycles Waste Manag 21, 239–247 (2019). https://doi.org/10.1007/s10163-018-0779-5

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Keywords

  • CaCO3
  • Heavy metal
  • Soil
  • Mine tailing
  • S/S
  • Indigenous bacteria