Abstract
This study investigated the production of d-lactic acid from unutilized sugarcane bagasse using steam explosion pretreatment. The optimal steam pressure for a steaming time of 5 min was determined. The steam-exploded sugarcane bagasse was hydrolyzed using cellulase (Meicelase) and then the hydrolyzate was subjected to fermentation substrate. By enzymatic saccharification using Meicelase, the highest recovery of glucose from raw bagasse, 73.7 %, was obtained at a steam pressure of 20 atm. For extracted residue with water after steam explosion, the glucose recovery increased up to 94.9 % at a steam pressure of 20 atm. These results showed that washing with water is effective in removing enzymatic reaction inhibitors. After steam pretreatment (steam pressure of 20 atm), d-lactic acid was produced by Lactobacillus delbrueckii NBRC 3534 from the enzymatic hydrolyzate of steam-exploded bagasse and washed residue. The conversion rate of d-lactic acid obtained from the glucose concentration was 66.6 % for the hydrolyzate of steam-exploded bagasse without washing with water and 90.0 % for that derived from the extracted residue with water after steam explosion. These results also demonstrated that the hydrolyzate of steam-exploded bagasse (without washing with water) contains fermentation inhibitors and washing with water can remove them.
Similar content being viewed by others
References
da Silva AS, Inoue H, Endo T, Yano S, Bon EPS (2010) Milling pretreatment of sugarcane bagasse and straw for enzymatic hydrolysis and ethanol fermentation. Bioresour Technol 101:7402–7409
Zhao X, Peng F, Cheng K, Liu D (2009) Enhancement of the enzymatic digestibility of sugarcane bagasse by alkali-peracetic acid pretreatment. Enzyme Microb Technol 44(1):17–23
Rocha GJM, Gonçalves AR, Oliveira BR, Olivares EG, Rossell CEV (2012) Steam explosion pretreatment reproduction and alkaline delignification reactions performed on a pilot scale with sugarcane bagasse for bioethanol production. Ind Crops Prod 35:274–279
Soares IB, Travassos JA, Baudel HM, Benachour M, Abreu CAM (2011) Effects of washing, milling and loading enzymes on the enzymatic hydrolysis of a steam pretreated sugarcane bagasse. Ind Crops Prod 33:670–675
Binod P, Satyanagalakshmi K, Sindhu R, Janu KU, Sukumaran RK, Pandey A (2012) Short duration microwave assisted pretreatment enhances the enzymatic saccharification and fermentable sugar yield from sugarcane bagasse. Renew Energy 37(1):109–116
Sasaki C, Takada R, Watanabe T, Honda Y, Karita S, Nakamura Y, Watanabe T (2011) Surface carbohydrate analysis and bioethanol production of sugarcane bagasse pretreated with the white rot fungus, Ceriporiopsis subvermispora and microwave hydrothermolysis. Bioresour Technol 102:9942–9946
Marques S, Santos JAL, Gírio FM, Roseiro JC (2008) Lactic acid production from recycled paper sludge by simultaneous saccharification and fermentation. Biochem Eng J 41:210–216
Nakasaki K, Adachi T (2003) Effects of intermittent addition of cellulase for production of l-lactic acid from wastewater sludge by simultaneous saccharification and fermentation. Biotechnol Bioeng 82:263–270
John RP, Nampoothiri KM, Pandey A (2006) Solid-state fermentation for l-lactic acid production from agro wastes using Lactobacillus delbrueckii. Process Biochem 41:759–763
Shindo S, Tachibana T (2004) Production of l-lactic acid from spent grain, a by-product of beer production. J Inst Brew 110:347–351
Ikeda Y, Jamshidi K, Tsuji H, Hyon SH (1987) Stereocomplex formation between enantiomeric poly(lactides). Macromolecules 20:904–906
Tsuji H, Horii F, Hyon SH, Ikada Y (1991) Stereocomplex formation between enantiomeric poly(lactic acid)s. 2. Stereocomplex formation in concentrated solutions. Macromolecules 24:2719–2724
Slivniak R, Domb AJ (2002) Stereocomplexes of enantiomeric lactic acid and sebacic acid ester-anhydride triblock copolymers. Biomacromolecules 3:754–760
Fukushima K, Sogo K, Miura S, Kimura Y (2004) Production of d-lactic acid by bacterial fermentation of rice starch. Macromol Biosci 4:1021–1027
Lu Z, Lu M, He F, Yu L (2009) An economical approach for d-lactic acid production utilizing unpolished rice from aging paddy as major nutrient source. Bioresour Technol 100:2026–2031
Chua MGS, Wayman M (1979) Characterization of autohydrolysis aspen (P. tremuloides) lignins. Part 1. Composition and molecular weight distribution of extracted autohydrolysis linin. Can J Chem 57:1141–1149
Mikami H, Ishida Y (1983) Post-column fluorometric detection of reducing sugars in high performance liquid chromatography using arginine (in Japanese). Bunseki Kagaku 32:E207–E210
Palmqvist E, Hahn-Hägerdal B (2000) Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition. Bioresour Technol 74:25–33
Ando H, Sakaki T, Kokusho T, Shibata M, Uemura Y, Hatate Y (2000) Decomposition behavior of plant biomass in hot-compressed water. Ind Eng Chem Res 39:3688–3693
Dunlop AP (1948) Furfural formation and behavior. Ind Eng Chem 40:204–209
Nakamura Y, Sawada T, Inoue E (2001) Enhanced ethanol production from enzymatically treated steam-exploded rice straw using extractive fermentation. J Chem Technol Biotechnol 76:879–884
Kobayashi F, Take H, Asada C, Nakamura Y (2004) Methane production from steam-exploded bamboo. J Biosci Bioeng 97:426–428
Asada C, Nakamura Y, Kobayashi F (2005) Chemical characteristics and ethanol fermentation of the cellulose component in autohydrolyzed bagasse. Biotechnol Bioprocess Eng 10:346–352
Larsson S, Palmqvist E, Hahn-Hägerdal B, Tengborg C, Stenberg K, Zacchi G, Nilvebrant N-O (1999) The generation of fermentation inhibitors during dilute acid hydrolysis of softwood. Enzyme Microb Technol 24:151–159
Helle S, Cameron D, Lam J, White B, Duff S (2003) Effect of inhibitory compounds found in biomass hydrolysates on growth and xylose fermentation by a genetically engineered strain of S. cerevisiae. Enzyme Microb Technol 33:786–792
Ruiz E, Cara C, Manzanares P, Ballesteros M, Castro E (2008) Evaluation of steam explosion pre-treatment for enzymatic hydrolysis of sunflower stalks. Enzyme Microb Technol 42:160–166
Caparrós S, Ariza J, López F, Nacimiento JA, Garrote G, Jiménez L (2008) Hydrothermal treatment and ethanol pulping of sunflower stalks. Bioresour Technol 99:1368–1372
Ulbricht RJ, Northup SJ, Thomas JA (1984) A review of 5-hydroxymethylfurfural (HMF) in parenteral solutions. Fundam Appl Toxicol 4:843–853
Bardet M, Robert DR (1985) On the reactions and degradation of the lignin during steam hydrolysis of aspen wood. Svensk Papperstidning 6:61–67
Lapierre C, Rolando C, Monties B (1983) Characterization of poplar lignins acidolysis products: capillary gas–liquid and liquid–liquid chromatography of monomeric compounds. Holzforschung 37:189–198
Delgenes JP, Moletta R, Navarro JM (1996) Effects of lignocellulose degradation products on ethanol fermentations of glucose and xylose by Saccharomyces cerevisiae, Zymomonas mobilis, Pichia stipitis, and Candida shehatae. Enzyme Microb Technol 19:220–225
Palmqvist E, Almeida JS, Hahn-Hägerdal B (1999) Influence of furfural on anaerobic glycolytic kinetics of Saccharomyces cerevisiae in batch culture. Biotechnol Bioeng 62:447–454
Kim Y, Ximenes E, Mosier NS, Ladisch MR (2011) Soluble inhibitors/deactivators of cellulase enzymes from lignocellulosic biomass. Enzyme Microb Technol 48:408–415
Highley TL (1975) Inhibition of cellulases of wood-decay fungi. USDA Forest Service Research Paper FPL 247:1–8
Mcallister TA, Bae HD, Yanke LJ, Cheng KJ, Muir A (1994) Effect of condensed tannins from birdsfoot trefoil on endoglucanase activity and the digestion of cellulose filter paper by ruminal fungi. Can J Microbiol 40:298–305
Sineiro J, Dominguez H, Núñez MJ, Lema JM (1997) Inhibition of cellulase activity by sunflower polyphenols. Biotechnol Lett 19:521–524
Acknowledgments
A part of this study was funded by the Shiseido Female Researcher Science Grant and a Grant-in-Aid for Young Scientists (B) (no. 21750159) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sasaki, C., Okumura, R., Asakawa, A. et al. Effects of washing with water on enzymatic saccharification and d-lactic acid production from steam-exploded sugarcane bagasse. J Mater Cycles Waste Manag 14, 234–240 (2012). https://doi.org/10.1007/s10163-012-0064-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10163-012-0064-y