During development, axonogenesis, an integral part of neurogenesis, is based on well-concerted events comprising generation, rearrangement, migration, elongation, and adhesion of neurons. Actin, specifically the crosstalk between the guardians of actin polymerization, like enabled, chickadee, capping protein plays an essential role in crafting several events of axonogenesis. Recent evidences reflect multifaceted role of microRNA during axonogenesis. Here, we investigated the role of bantam miRNA, a well-established miRNA in Drosophila, in regulating the actin organization during brain development. Our immunofluorescence studies showed altered arrangement of neurons and actin filaments whereas both qPCR and western blot revealed elevated expression of enabled, one of the actin modulators in bantam mutant background. Collectively, our results clearly demonstrate that bantam plays an instrumental role in shaping the axon architecture regulating the actin geometry through its modulator enabled.
DrosophilamiRNA Bantam Axon Actin Enabled
This is a preview of subscription content, log in to check access.
Compliance with ethical standards
Conflict of interest
Banerjee A, Roy JK (2017) Dicer-1 regulates proliferative potential of Drosophila larval neural stem cells through bantam miRNA based downregulation of the G1/S inhibitor Dacapo. Dev Biol 423:57–65CrossRefPubMedGoogle Scholar
Barzik M, Kotova TI, Higgs HN, Hazelwood L, Hanein D, Gertler FB, Schafer DA (2005) Ena/VASP proteins enhance actin polymerization in the presence of barbed end capping proteins. J Biol Chem 280:28653–28662CrossRefPubMedPubMedCentralGoogle Scholar
Bashaw GJ, Kidd T, Murray D, Pawson T, Goodman CS (2000) Repulsive axon guidance: abelson and enabled play opposing roles downstream of the roundabout receptor. Cell 101:703–715CrossRefPubMedGoogle Scholar
Bear JE, Loureiro JJ, Libova I, Fassler R, Wehland J, Gertler FB (2000) Negative regulation of fibroblast motility by Ena/VASP proteins. Cell 101:717–728CrossRefPubMedGoogle Scholar
Becam I, Rafel N, Hong X, Cohen SM, Milan M (2011) Notch-mediated repression of bantam miRNA contributes to boundary formation in the Drosophila wing. Development 138:3781–3789CrossRefPubMedGoogle Scholar
Bentley D, Toroian-Raymond A (1986) Disoriented pathfinding by pioneer neurone growth cones deprived of filopodia by cytochalasin treatment. Nature 323:712–715CrossRefPubMedGoogle Scholar
Brennecke J, Hipfner DR, Stark A, Russell RB, Cohen SM (2003) bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. Cell 113:25–36CrossRefPubMedGoogle Scholar
Lebrand C, Dent EW, Strasser GA, Lanier LM, Krause M, Svitkina TM, Borisy GG, Gertler FB (2004) Critical role of Ena/VASP proteins for filopodia formation in neurons and in function downstream of netrin-1. Neuron 42:37–49CrossRefPubMedGoogle Scholar
Neumüller RA, Richter C, Fischer A, Novatchkova M et al (2011) Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. Cell Stem Cell 8:580–593CrossRefPubMedPubMedCentralGoogle Scholar