Advertisement

Bantam regulates the axonal geometry of Drosophila larval brain by modulating actin regulator enabled

Short Communication

Abstract

During development, axonogenesis, an integral part of neurogenesis, is based on well-concerted events comprising generation, rearrangement, migration, elongation, and adhesion of neurons. Actin, specifically the crosstalk between the guardians of actin polymerization, like enabled, chickadee, capping protein plays an essential role in crafting several events of axonogenesis. Recent evidences reflect multifaceted role of microRNA during axonogenesis. Here, we investigated the role of bantam miRNA, a well-established miRNA in Drosophila, in regulating the actin organization during brain development. Our immunofluorescence studies showed altered arrangement of neurons and actin filaments whereas both qPCR and western blot revealed elevated expression of enabled, one of the actin modulators in bantam mutant background. Collectively, our results clearly demonstrate that bantam plays an instrumental role in shaping the axon architecture regulating the actin geometry through its modulator enabled.

Keywords

Drosophila miRNA Bantam Axon Actin Enabled 

Notes

Compliance with ethical standards

Conflict of interest

None.

References

  1. Banerjee A, Roy JK (2017) Dicer-1 regulates proliferative potential of Drosophila larval neural stem cells through bantam miRNA based downregulation of the G1/S inhibitor Dacapo. Dev Biol 423:57–65CrossRefPubMedGoogle Scholar
  2. Barzik M, Kotova TI, Higgs HN, Hazelwood L, Hanein D, Gertler FB, Schafer DA (2005) Ena/VASP proteins enhance actin polymerization in the presence of barbed end capping proteins. J Biol Chem 280:28653–28662CrossRefPubMedPubMedCentralGoogle Scholar
  3. Bashaw GJ, Kidd T, Murray D, Pawson T, Goodman CS (2000) Repulsive axon guidance: abelson and enabled play opposing roles downstream of the roundabout receptor. Cell 101:703–715CrossRefPubMedGoogle Scholar
  4. Bear JE, Loureiro JJ, Libova I, Fassler R, Wehland J, Gertler FB (2000) Negative regulation of fibroblast motility by Ena/VASP proteins. Cell 101:717–728CrossRefPubMedGoogle Scholar
  5. Becam I, Rafel N, Hong X, Cohen SM, Milan M (2011) Notch-mediated repression of bantam miRNA contributes to boundary formation in the Drosophila wing. Development 138:3781–3789CrossRefPubMedGoogle Scholar
  6. Bentley D, Toroian-Raymond A (1986) Disoriented pathfinding by pioneer neurone growth cones deprived of filopodia by cytochalasin treatment. Nature 323:712–715CrossRefPubMedGoogle Scholar
  7. Berdnik D, Fan AP, Potter CJ, Luo L (2008) microRNA processing pathway regulates olfactory neuron morphogenesis. Curr Biol 25:1754–1759.  https://doi.org/10.1016/j.cub.2008.09.045 CrossRefGoogle Scholar
  8. Brennecke J, Hipfner DR, Stark A, Russell RB, Cohen SM (2003) bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. Cell 113:25–36CrossRefPubMedGoogle Scholar
  9. Davis GM, Haas MA, Pocock R (2015) MicroRNAs: not “fine-tuners” but key regulators of neuronal development and function. Front Neurol 6:245.  https://doi.org/10.3389/fneur.2015.00245 CrossRefPubMedPubMedCentralGoogle Scholar
  10. Dent EW, Gupton SL, Gertler FB (2011) The growth cone cytoskeleton in axon outgrowth and guidance. Cold Spring Harb Perspect Biol 3:a001800CrossRefPubMedPubMedCentralGoogle Scholar
  11. Hipfner DR, Weigmann K, Cohen SM (2002) The bantam gene regulates Drosophila growth. Genetics 161:1527–1537PubMedPubMedCentralGoogle Scholar
  12. Homem CCF, Knoblich JA (2012) Drosophila neuroblasts: a model for stem cell biology. Development 139:4297–4310CrossRefPubMedGoogle Scholar
  13. Kosik KS (2006) The neuronal microRNA system. Nat Rev Neurosci 7:911–920CrossRefPubMedGoogle Scholar
  14. Lebrand C, Dent EW, Strasser GA, Lanier LM, Krause M, Svitkina TM, Borisy GG, Gertler FB (2004) Critical role of Ena/VASP proteins for filopodia formation in neurons and in function downstream of netrin-1. Neuron 42:37–49CrossRefPubMedGoogle Scholar
  15. Lu CS, Zhai B, Mauss A, Landgraf M, Gygi S, Vactor DV (2014) MicroRNA-8 promotes robust motor axon targeting by coordinate regulation of cell adhesion molecules during synapse development. Philos Trans R Soc B 369:20130517.  https://doi.org/10.1098/rstb.2013.0517 CrossRefGoogle Scholar
  16. Luhur A, Chawla G, Wu Y, Li J, Sokol NS (2014) Drosha-independent DGCR8/Pasha pathway regulates neuronal morphogenesis. PNAS 111:1421–1426CrossRefPubMedPubMedCentralGoogle Scholar
  17. Nawabi H, Zukor K, He Z (2012) No simpler than mammals: axon and dendrite regeneration in Drosophila. Genes Dev 26:1509–1514CrossRefPubMedPubMedCentralGoogle Scholar
  18. Neumüller RA, Richter C, Fischer A, Novatchkova M et al (2011) Genome-wide analysis of self-renewal in Drosophila neural stem cells by transgenic RNAi. Cell Stem Cell 8:580–593CrossRefPubMedPubMedCentralGoogle Scholar
  19. Singh AK, Lakhotia SC (2015) Dynamics of hnRNPs and omega speckles in normal and heat shocked live cell nuclei of Drosophila melanogaster. Chromosoma.  https://doi.org/10.1007/s00412-015-0506-0 Google Scholar
  20. Tessier-Lavigne M, Goodman CS (1996) The molecular biology of axon guidance. Science 274:1123–1133CrossRefPubMedGoogle Scholar
  21. Vidigal JA, Ventura A (2015) The biological functions of miRNAs: lessons from in vivo studies. Trends Cell Biol 25:137–147CrossRefPubMedGoogle Scholar
  22. Wear MA, Cooper JA (2004) Capping protein: new insights into mechanism and regulation. Trends Biochem Sci 29:418–428CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Cytogenetics Laboratory, Department of ZoologyBanaras Hindu UniversityVaranasiIndia

Personalised recommendations