Abstract
The prevalence of strains with ampicillin (ABPC) resistance among Haemophilus influenzae strains isolated from the nasopharynx of children with lower respiratory tract infections has increased significantly during the 6 years from 2000, when it was 41.9%, to 2005, when it reached 60.1%. From 2002, the prevalence exceeded 50%, and the prevalence of β-lactamase-nonproducing ABPC-resistant (BLNAR) strains with a minimum inhibitory concentration (MIC) of ABPC of over 4 µg/ml doubled, from 28.2% in 2002 to 54.7% in 2005. In H. influenzae strains obtained from the nasopharynx of children with lower respiratory tract infections between April 2004 and March 2006, identification of serotype b was defined, using the slide agglutination method. The frequency of isolation of H. influenzae type b (Hib) strains was then measured and the ABPC resistance conditions of the Hib strains were also evaluated. The frequency of the Hib strains was found to be 30 out of 479 strains, 6.3%. Of these 30 strains, BLNAR accounted for 53.3% (16 strains), approximately the same frequency of isolation as that of the BLNAR isolated from all H. influenzae strains during the same period. In Japan, the prevalence of BLNAR strains among clinically isolated H. influenzae strains has continued to increase, and the frequency of isolation of BLNAR strains among Hib strains has also continued to rise. As a countermeasure, attempts at improving resistance have been made through judicious antibiotic use, but concern that the choice of antibiotics for Hib meningitis may become complicated has sparked a keen interest in the introduction of Hib conjugate vaccine.
Similar content being viewed by others
References
H Seki Y Kasahara K Ohta K Ohta Y Saikawa R Sumita et al. (1999) ArticleTitleIncreasing prevalence of ampicillin-resistant non-beta-lactamase-producing strains of Haemophilus influenzae in children in Japan Chemotherapy 45 15–21 Occurrence Handle9876205 Occurrence Handle10.1159/000007160 Occurrence Handle1:CAS:528:DyaK1MXhtFajsLk%3D
K Sunakawa M Nonoyama T Ooishi S Iwata H Akita Y Sato et al. (2006) ArticleTitleTrends in pediatric bacterial meningitis in Japan (2003–2004) J Infect Chemother 80 27–38
A Nakamura N Ishikawa H Suzuki T Kurosaki I Terashima S Uehara et al. (1995) ArticleTitleClinical bacteriology of Haemophilus influenzae isolated from respiratory tract infection in children J Jpn Pediatr Soc 99 1234–41
K Ubukata Y Shibasaki K Yamamoto N Chiba K Hasegawa Y Takeuchi et al. (2001) ArticleTitleAssociation of amino acid substitutions in penicillin-binding proteins 3 with beta-lactam resistance in β-lactamase-negative ampicillin-resistant Haemophilus influenzae Antimicrob Agents Chemother 45 1963–6 Occurrence Handle10.1128/AAC.45.6.1693-1699.2001
H Kuroki N Ishikawa S Uehara K Himi T Sonobe H Niimi (1997) ArticleTitleNasopharyngeal colonization with Haemophilus influenzae type b among infants and children in Japan Acta Paediatr Jpn 39 541–5 Occurrence Handle9363649 Occurrence Handle1:STN:280:DyaK1c%2FjtVWjsg%3D%3D
A Nariai Y Kobayashi (2005) ArticleTitleThe isolation rate of Haemophilus influenzae type b among Haemophilus influenzae strains obtained from nasopharynx of children with lower respiratory tract infections J Jpn Pediatr Soc 109 726–9
K Funabashi M Ushigaki K Nakane K Tanaka N Nishimura T Ozaki (2001) ArticleTitleSerotypes and antimicrobial sensitivities of strains of Haemophilus influenzae isolated from pharyngeal and mycteric swabs of children Jpn J Med Tech 50 22–6
Author information
Authors and Affiliations
Corresponding author
Additional information
Part of this report was presented at the fifty-fourth general meeting of the Japanese Society of Chemotherapy (Kyoto, May 2006).
About this article
Cite this article
Nariai, A. Prevalence of β-lactamase-nonproducing ampicillin-resistant Haemophilus influenzae and Haemophilus influenzae type b strains obtained from children with lower respiratory tract infections. J Infect Chemother 13, 396–399 (2007). https://doi.org/10.1007/s10156-007-0564-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10156-007-0564-1