Abstract
To investigate the role of matrix metalloproteinases (MMPs) in the mobilization of peripheral blood stem cells stimulated by granulocyte colony-stimulating factor (G-CSF), we analyzed MMP serum levels in 11 healthy donors and 9 patients who had hematological malignancies or germ cell tumors. A dose of 5–10 µg/kg per day of G-CSF (lenograstim) was administered for 4–8 days to each subject. The serum levels of MMP-2, and MMP-9; interleukin-3, -6, -8, and -10; stem cell factor; interferon-γ; and tumor necrosis factor-α were measured both before and during G-CSF administration. MMP-9 was found to be increased in both the cancer patients and the healthy donor group. In contrast, the levels of each of the other factors tested were unchanged. No significant positive correlation was observed between the MMP-9 levels and the number of CD34+ cells. Hence, we found no significant role for MMPs during the mobilization of peripheral blood stem cells stimulated by G-CSF.
Similar content being viewed by others
References
EJ Goetzl MJ Banda D Leppert (1996) ArticleTitleMatrix metalloproteinase in immunity J Immunol 156 1–4 Occurrence Handle8598448 Occurrence Handle1:CAS:528:DyaK28XlsVCq
L Laterveer IJ Lindley DP Heemskerk JA Camps EK Pauwels R Willemze et al. (1996) ArticleTitleRapid mobilization of hematopoietic progenitor cells in rhesus monkeys by a single intravenous injection of interleukin-8 Blood 87 781–8 Occurrence Handle8555503 Occurrence Handle1:CAS:528:DyaK28XkvFWitA%3D%3D
JF Pruijt WE Fibbe L Laterveer RA Pieters IJ Lindly L Paeman et al. (1999) ArticleTitlePrevention of interleukin-8-induced mobilization of hematopoietic progenitor cells in rhesus monkeys by inhibitory antibodies against the metalloproteinase gelatinase B (MMP-9) Proc Natl Acad Sci U S A 96 IssueID10 863–8
T Watanabe Y Kawano S Kanamaru T Onishi S Kaneko Y Wakata et al. (1999) ArticleTitleEndogenous interleukin-8 (IL-8) surge in granulocyte colony-stimulating factor-induced peripheral blood stem cell mobilization Blood 94 1157–63
B Heissig K Hattori S Dias M Friedrich B Ferris NR Hackett et al. (2002) ArticleTitleRecruitment of stem and progenitor cells from the bone marrow niche requires MMP-9 mediated release of Kit-ligand Cell 109 625–37 Occurrence Handle12062105 Occurrence Handle10.1016/S0092-8674(02)00754-7 Occurrence Handle1:CAS:528:DC%2BD38XksVGgs7w%3D
LM Pelus H Bian AG King S Fukuda (2004) ArticleTitleNeutrophil-derived MMP-9 mediates synergistic mobilization of hematopoietic stem and progenitor cells by the combination of G-CSF and the chemokines GROβ/CXCL2 and GROβT/CXCL2Δ4 Blood 103 110–9 Occurrence Handle12958067 Occurrence Handle10.1182/blood-2003-04-1115 Occurrence Handle1:CAS:528:DC%2BD2cXhsFCqug%3D%3D
D Carstanjen N Ulbricht A Iacone M Regenfus A Salama (2002) ArticleTitleMatrix metalloproteinase-9 (gelatinase B) is elevated during mobilization of peripheral blood progenitor cells by G-CSF Transfusion 42 588–96 Occurrence Handle12084167 Occurrence Handle10.1046/j.1537-2995.2002.00088.x Occurrence Handle1:CAS:528:DC%2BD38Xlt1Kgs7o%3D
A Carion L Benboubker O Herault F Roingeard M Degenne D Senecal et al. (2003) ArticleTitleStromal-derived factor 1 and matrix metalloproteinase 9 levels in bone marrow and peripheral blood of patients mobilized by granulocyte colony-stimulating factor and chemotherapy. Relationship with mobilizing capacity of haematopoietic progenitor cells Br J Haematol 122 918–26 Occurrence Handle12956762 Occurrence Handle10.1046/j.1365-2141.2003.04545.x Occurrence Handle1:CAS:528:DC%2BD3sXotFKns74%3D
M Xu E Bruno J Chao S Huang G Finazzi SM Fruchtman et al. (2005) ArticleTitleConstitutive mobilization of CD34+ cells into the peripheral blood in idiopathic myelofibrosis may be due to the action of a number of proteases Blood 105 4508–15 Occurrence Handle15705794 Occurrence Handle10.1182/blood-2004-08-3238 Occurrence Handle1:CAS:528:DC%2BD2MXks12isbs%3D
D Domanović G Wozniak P Cernelc M Samardzija S Balen-Marunić P Rozman (2005) ArticleTitleMatrix metalloproteinase-9 and cell kinetics during the collection of peripheral blood stem cells by leukapheresis Transfus Apher Sci 33 37–45 Occurrence Handle15951240 Occurrence Handle10.1016/j.transci.2004.09.009
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Saito, T., Usui, N., Asai, O. et al. Elevated serum levels of human matrix metalloproteinase-9 (MMP-9) during the induction of peripheral blood stem cell mobilization by granulocyte colony-stimulating factor (G-CSF). J Infect Chemother 13, 426–428 (2007). https://doi.org/10.1007/s10156-007-0553-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10156-007-0553-4