Skip to main content

Utility of a rapid diagnosis kit for Mycoplasma pneumoniae pneumonia in children, and the antimicrobial susceptibility of the isolates

Abstract

We evaluated a kit for the rapid diagnosis of Mycoplasma pneumoniae infection and investigated the antimicrobial susceptibility of the isolates. A total of 194 otherwise healthy children, aged 0.3–14.9 years, were diagnosed as having pneumonia by chest X-ray findings between December 2003 and November 2004, and were admitted to Showa Hospital. Isolation of M. pneumoniae was attempted from a throat swab obtained on admission, and the complement fixation titer was measured in paired serum samples obtained at admission and at the convalescent stage. We also used a rapid diagnosis kit (ImmunoCard Mycoplasma) for the detection of specific immunoglobulin M antibody in paired sera. Pneumonia due to M. pneumoniae was defined by isolation of this microorganism, or by seroconversion, or a ≥4-fold increase in the antibody titer. Using each isolate, we determined the minimum inhibitory concentrations for five antimicrobial agents by the broth dilution method. M. pneumoniae pneumonia was diagnosed in 45 children (23.2%). The ImmunoCard had a sensitivity of 31.8% using admission serum and 88.6% using paired sera, while the specificity was 78.1% and 70.5%, respectively. M. pneumoniae was isolated from 14 of the 45 patients (31.1%). The 50%/90% minimum inhibitory concentration (µg/ml) of erythromycin, clarithromycin, azithromycin, minocycline, and levofloxacin was 0.006/0.012, ≤0.003/≤0.003, ≤0.003/≤0.003, 0.78/1.56, and 0.39/0.78, respectively. For a rapid diagnosis of M. pneumoniae pneumonia, the ImmunoCard was not effective. Macrolides showed superior in vitro antimicrobial activity against M. pneumoniae isolates causing pediatric pneumonia.

This is a preview of subscription content, access via your institution.

References

  1. TS Alexander LD Gray JA Kraft DS Leland MT Nikaido DH Willis (1996) ArticleTitlePerformance of Meridian ImmunoCard Mycoplasma test in a multicenter clinical trial J Clin Microbiol 34 1180–3 Occurrence Handle8727899 Occurrence Handle1:STN:280:BymB1cvkt1Y%3D

    PubMed  CAS  Google Scholar 

  2. L Matas J Dominguez F De Ory N Garcia N Gali PJ Cardona et al. (1998) ArticleTitleEvaluation of Meridian ImmunoCard Mycoplasma test for the detection of Mycoplasma pneumoniae-specific IgM in paediatric patients Scand J Infect Dis 30 289–93 Occurrence Handle9790139 Occurrence Handle10.1080/00365549850160954 Occurrence Handle1:STN:280:DyaK1M%2FgsVOquw%3D%3D

    PubMed  Article  CAS  Google Scholar 

  3. N Okazaki M Narita S Yamada K Izumikawa M Umetsu T Kenri et al. (2001) ArticleTitleCharacteristics of macrolide-resistant Mycoplasma pneumoniae strains isolated from patients and induced with erythromycin in vivo Microbiol Immunol 45 617–20 Occurrence Handle11592636 Occurrence Handle1:CAS:528:DC%2BD3MXmvFWgtL8%3D

    PubMed  CAS  Google Scholar 

  4. M Matsuoka M Narita N Okazaki H Ohya T Yamazaki K Ouchi et al. (2004) ArticleTitleCharacterization and molecular analysis of macrolide-resistant Mycoplasma pneumoniae clinical isolates obtained in Japan Antimicrob Agents Chemother 48 4624–30 Occurrence Handle15561835 Occurrence Handle10.1128/AAC.48.12.4624-4630.2004 Occurrence Handle1:CAS:528:DC%2BD2cXhtVKmtrbJ

    PubMed  Article  CAS  Google Scholar 

  5. M Morozumi K Hasegawa R Kobayashi N Inoue S Iwata H Kuroki et al. (2005) ArticleTitleEmergence of macrolide-resistant Mycoplasma pneumoniae with a 23S rRNA gene mutation Antimicrob Agents Chemother 49 2302–6 Occurrence Handle15917525 Occurrence Handle10.1128/AAC.49.6.2302-2306.2005 Occurrence Handle1:CAS:528:DC%2BD2MXkvFCgsr0%3D

    PubMed  Article  CAS  Google Scholar 

  6. S Suzuki T Yamazaki M Narita N Okazaki I Suzuki T Andoh et al. (2006) ArticleTitleClinical evaluation of macrolide-resistant Mycoplasma pneumoniae Antimicrob Agents Chemother 50 709–12 Occurrence Handle16436730 Occurrence Handle10.1128/AAC.50.2.709-712.2006 Occurrence Handle1:CAS:528:DC%2BD28XhsFegur0%3D

    PubMed  Article  CAS  Google Scholar 

  7. CM Bébéar I Kempf (2005) Antimicrobial therapy and antimicrobial resistance A Blanchard G Browning (Eds) Mycoplasmas Horizon Bioscience Norfolk 535–68

    Google Scholar 

  8. DA Powell (2004) Mycoplasmal infections RE Behrman RM Kliegman HB Jenson (Eds) Nelson textbook of pediatrics EditionNumber17th ed Saunders Pennsylvania 990–2

    Google Scholar 

  9. N Miyashita H Fukano K Mouri M Fukuda K Yoshida Y Kobashi et al. (2005) ArticleTitleCommunity-acquired pneumonia in Japan: a prospective ambulatory and hospitalized patient study J Med Microbiol 54 395–400 Occurrence Handle15770027 Occurrence Handle10.1099/jmm.0.45920-0

    PubMed  Article  Google Scholar 

  10. K Ouchi H Komura M Fujii H Matsushima T Maki K Hasegawa et al. (1999) ArticleTitleChlamydia pneumoniae infection and Mycoplasma pneumoniae infection in pediatric patients Kansenshogaku Zasshi 73 1177–82 Occurrence Handle10655676 Occurrence Handle1:STN:280:DC%2BD3c7itFWhuw%3D%3D

    PubMed  CAS  Google Scholar 

  11. Y Miyajima H Kidokoro Y Sato M Suzuki T Kubota T Kato et al. (2000) ArticleTitle Chlamydia pneumoniae and Mycoplasma pneumoniae in children with respiratory tract infections J Jpn Pediatr Soc 104 723–9

    Google Scholar 

  12. A Nariai (2004) ArticleTitle Mycoplasma pneumoniae infection in hospitalized children with acute pneumonia under the Mycoplasma epidemic Kansenshogaku Zasshi 78 496–502 Occurrence Handle15287477

    PubMed  Google Scholar 

  13. M Morozumi K Hasegawa N Chiba S Iwata N Kawamura H Kuroki et al. (2004) ArticleTitleApplication of PCR for Mycoplasma pneumoniae detection in children with community-acquired pneumonia J Infect Chemother 10 274–9 Occurrence Handle16163461 Occurrence Handle10.1007/s10156-004-0338-y Occurrence Handle1:CAS:528:DC%2BD2cXps1Onu7k%3D

    PubMed  Article  CAS  Google Scholar 

  14. M Morozumi A Ito SY Murayama K Hasegawa R Kobayashi S Iwata et al. (2006) ArticleTitleAssessment of real-time PCR for diagnosis of Mycoplasma pneumoniae pneumonia in pediatric patients Can J Microbiol 52 125–9 Occurrence Handle16541148 Occurrence Handle10.1139/w05-118 Occurrence Handle1:CAS:528:DC%2BD28XksVWltbY%3D

    PubMed  Article  CAS  Google Scholar 

  15. M Narita (2005) ArticleTitleEvaluation of ELISA kits for detection of Mycoplasma pneumoniae-specific IgG, IgA, IgM antibodies on the diagnosis of Mycoplasma pneumoniae infection in children Kansenshogaku Zasshi 79 457–63 Occurrence Handle16119804

    PubMed  Google Scholar 

  16. InstitutionalAuthorNameAmerican Academy of Pediatrics (2006) Mycoplasma pneumoniae infections LK Pickering CJ Baker SS Long JA McMillan (Eds) Red book: 2006 report of the Committee on Infectious Diseases EditionNumber27th ed American Academy of Pediatrics Illinois 468–70

    Google Scholar 

  17. D Taylor-Robinson C Bébéar (1997) ArticleTitleAntibiotic susceptibilities of mycoplasmas and treatment of mycoplasmal infections J Antimicrob Chemother 40 622–30 Occurrence Handle9421309 Occurrence Handle10.1093/jac/40.5.622 Occurrence Handle1:CAS:528:DyaK2sXnvFWis70%3D

    PubMed  Article  CAS  Google Scholar 

  18. KB Waites GH Cassell KC Canupp PB Fernandes (1998) ArticleTitleIn vitro susceptibilities of mycoplasmas and ureaplasmas to new macrolides and aryl-fluoroquinolones Antimicrob Agents Chemother 32 1500–2

    Google Scholar 

  19. KB Wattes DM Crabb LB Duffy (2003) ArticleTitleInhibitory and bactericidal activities of gemifloxacin and other antimicrobials against Mycoplasma pneumoniae Int J Antimicrob Agents 21 574–7 Occurrence Handle10.1016/S0924-8579(03)00060-8

    Article  Google Scholar 

  20. KB Waites (2003) ArticleTitleNew concepts of Mycoplasma pneumoniae infections in children Pediatr Pulmonol 36 267–78 Occurrence Handle12950038 Occurrence Handle10.1002/ppul.10346

    PubMed  Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Takao Ozaki.

About this article

Cite this article

Ozaki, T., Nishimura, N., Ahn, J. et al. Utility of a rapid diagnosis kit for Mycoplasma pneumoniae pneumonia in children, and the antimicrobial susceptibility of the isolates. J Infect Chemother 13, 204–207 (2007). https://doi.org/10.1007/s10156-007-0519-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10156-007-0519-6

Key words

  • Mycoplasma pneumoniae
  • Pneumonia
  • Rapid diagnosis
  • Antimicrobial susceptibility
  • Macrolides