Skip to main content

Neutralizing activity of bovine colostral antibody against verotoxin derived from enterohemorrhagic Escherichia coli O157:H7 in mice

Abstract

The neutralization efficacy of bovine colostral antibody against verotoxin (VT) 1 and 2 was investigated. Cows were immunized with VT1 or VT2 fourteen times at 7-day intervals. A colostral antibody exhibiting high titers was obtained from immunized cows. Survival rates were evaluated in mice administered VT1 or VT2, and those infected with Escherichia coli (E. coli) O157:H7 producing VT1 or VT2. Survival rates after VT1 administration were 100% in the single-administration group, 90% in the repeat-administration group, and 78.6% in the control group. Survival rates after VT2 were 75.0% in the single-administration group, and 100% in the repeat-administration group. All mice in the control group died. Colostral antibody and fosfomycin (FOM) in the colostral antibody group and FOM and skim milk in the control group were administered three times per day for 5 days to mice infected with E. coli O157:H7 producing VT1 or VT2. Survival rates after inoculation with E. coli O157:H7 producing VT1 were 80.0% in the colostral antibody group, and 63.6% in the control group. Survival rates after inoculation with E. coli O157:H7 producing VT2 were 83.3% in the colostral antibody group, and 20.0% in the control group. The survival rate in mice without treatment following inoculation with E. coli O157:H7 producing VT2 was 88.2%.The survival rates in mice infected with E. coli O157:H7 strains producing VT1 or VT2 improved after administration of this colostral antibody, which exhibited neutralization efficacy against VT.

This is a preview of subscription content, access via your institution.

References

  1. MA Karmali (1989) ArticleTitleInfection by verocytoxin-producing Escherichia coli Clin Microbiol Rev 2 15–38 Occurrence Handle2644022 Occurrence Handle1:STN:280:DyaL1M7hvFCisQ%3D%3D

    PubMed  CAS  Google Scholar 

  2. J Nataro JB Kaper (1998) ArticleTitleDiarrheagenic Escherichia coli Clin Microbiol Rev 11 142–201 Occurrence Handle9457432 Occurrence Handle1:CAS:528:DyaK1cXntVGmsA%3D%3D

    PubMed  CAS  Google Scholar 

  3. H Michino K Araki S Minani S Takaya N Sakai M Miyazaki et al. (1999) ArticleTitleMassive outbreak of Escherichia coli O157:H7 infection in schoolchildren in Sakai City, Japan, associated with consumption of white radish sprouts Am J Epidemiol 150 787–96 Occurrence Handle10522649 Occurrence Handle1:STN:280:DyaK1MvltVCrtg%3D%3D

    PubMed  CAS  Google Scholar 

  4. JR Brandt LS Fouser SL Watkins I Zelikovic PI Tarr VN Stewart et al. (1994) ArticleTitleEscherichia coli O157:H7-associated hemolytic-uremic syndrome after ingestion of contaminated hamburgers J Pediatr 125 519–26 Occurrence Handle7931869 Occurrence Handle1:STN:280:DyaK2M%2FhslehtA%3D%3D Occurrence Handle10.1016/S0022-3476(94)70002-8

    PubMed  CAS  Article  Google Scholar 

  5. AO Carter AA Borczyk JAK Carlson B Harvey JC Hockin MA Karmali et al. (1987) ArticleTitleA severe outbreak of Escherichia coli O157:H7-associated hemorrhagic colitis in a nursing home N Engl J Med 317 1496–500 Occurrence Handle3317047 Occurrence Handle1:STN:280:DyaL1c%2FmsFOntw%3D%3D Occurrence Handle10.1056/NEJM198712103172403

    PubMed  CAS  Article  Google Scholar 

  6. TG Cleary (2004) ArticleTitleThe role of Shiga-toxin-producing Escherichia coli in hemorrhagic colitis and hemolytic uremic syndrome Semin Pediatr Infect Dis 14 260–5 Occurrence Handle10.1053/j.spid.2004.07.007

    Article  Google Scholar 

  7. H Karch PI Tarr M Bielaszewska (2005) ArticleTitleEnterohaemorrhagic Escherichia coli in human medicine Int J Med Microbiol 295 405–18 Occurrence Handle16238016 Occurrence Handle1:CAS:528:DC%2BD2MXht1Cqs73N Occurrence Handle10.1016/j.ijmm.2005.06.009

    PubMed  CAS  Article  Google Scholar 

  8. AT Pavia CR Nichols DP Green RV Tauxe S Mottice KD Greene et al. (1990) ArticleTitleHemolytic-uremic syndrome during an outbreak of Escherichia coli O157:H7 infections in mentally retarded persons: clinical and epidemiologic observations J Pediatr 116 544–51 Occurrence Handle2181098 Occurrence Handle1:STN:280:DyaK3c3gsVWmtw%3D%3D Occurrence Handle10.1016/S0022-3476(05)81600-2

    PubMed  CAS  Article  Google Scholar 

  9. CS Wong S Jelacic RL Hareeb SL Watkins PI Tarr (2000) ArticleTitleThe risk of the hemolytic-uremic syndrome after antibiotic treatment of Escherichia coli O157:H7 infections N Engl J Med 342 1930–6 Occurrence Handle10874060 Occurrence Handle1:STN:280:DC%2BD3czit1arsg%3D%3D Occurrence Handle10.1056/NEJM200006293422601

    PubMed  CAS  Article  Google Scholar 

  10. H Baum R Marre (2005) ArticleTitleAntimicrobial resistance of Escherichia coli and therapeutic implications Int J Med Microbiol 295 503–11 Occurrence Handle10.1016/j.ijmm.2005.07.002 Occurrence Handle1:CAS:528:DC%2BD2MXht1Cqs7rK

    Article  CAS  Google Scholar 

  11. S Higami K Nishimoto T Kawamura T Tsuruhara G Isshiki A Ookita (1998) ArticleTitleRetrospective analysis of the relationship between HUS incidence and antibiotics among patients with Escherichia coli O157 enterocolitis in the Sakai outbreak Kansenshogaku Zasshi 72 266–72 Occurrence Handle9643979 Occurrence Handle1:STN:280:DyaK1c3ks1OqsA%3D%3D

    PubMed  CAS  Google Scholar 

  12. T Ito E Akino K Hiramatsu (1997) ArticleTitleEvaluation of antibiotics used for enterohemorrhagic Escherichia coli O157 enteritis: effect of various antibiotics on extracellular release of verotoxin Kansennshogaku Zasshi 71 130–5 Occurrence Handle1:STN:280:DyaK2s3jtFOqsQ%3D%3D

    CAS  Google Scholar 

  13. B Phillips K Tryerman SM Whiteley (2005) ArticleTitleUse of antibiotics in suspected haemolytic-uraemic syndrome BMJ 330 409–11 Occurrence Handle15718542 Occurrence Handle10.1136/bmj.330.7488.409

    PubMed  Article  Google Scholar 

  14. PI Tarr (1991) ArticleTitleEscherichia coli O157:H7: clinical, diagnostic, and epidemiological aspects of human infection Clin Infect Dis 20 1–10

    Google Scholar 

  15. PI Tarr CA Gordon W Chandler (2005) ArticleTitleShiga-toxin-producing Escherichia coli and haemolytic uraemic syndrome Lancet 365 1073–86 Occurrence Handle15781103 Occurrence Handle1:CAS:528:DC%2BD2MXisFGrs7Y%3D

    PubMed  CAS  Google Scholar 

  16. JN Walterspiel AshkenaziS AL Morrow TG Cleary (1992) ArticleTitleEffect of subinhibitory concentrations of antibiotics on extracellular Shiga-like toxin I. Infection 20 25–33 Occurrence Handle1:CAS:528:DyaK38XisFWqur8%3D Occurrence Handle10.1007/BF01704889

    CAS  Article  Google Scholar 

  17. GD Armstrong E Fodor R Vanmaele (1991) ArticleTitleInvestigation of Shiga-like toxin binding to chemically synthesized oligosaccharide sequence J Infect Dis 64 1160–7

    Google Scholar 

  18. GD Armstrong PC Rowe P Goodyer E Orrbine TP Klassen G Wells et al. (1995) ArticleTitleA phase I study of chemically synthesized verotoxin (Shiga-like toxin) Pk-trisaccharide receptors attached to chromosorb for preventing hemolytic-uremic syndrome J Infect Dis 171 1042–5 Occurrence Handle7706786 Occurrence Handle1:STN:280:DyaK2M3itF2lsw%3D%3D

    PubMed  CAS  Google Scholar 

  19. T Takeda K Yoshino E Adachi K Yamagata H Uchida K Okonogi et al. (1998) ArticleTitleIn vitro specific binding of Shiga toxin 1 and 2 by TAK-751S (Gb3 analog) Kansenshogaku Zassshi 72 924–34 Occurrence Handle1:STN:280:DyaK1M%2Fht1artg%3D%3D

    CAS  Google Scholar 

  20. Y Matsumoto (2002) ArticleTitleAnti-Shiga-like toxin II (SLT-II) humanized monoclonal antibody Nippon Rinsho 60 473–9 Occurrence Handle11904961

    PubMed  Google Scholar 

  21. S Yamagishi M Mtoki T Kimura H Izumi T Takeda Y Katsuura et al. (2001) ArticleTitleEfficacy of postinfection treatment with anti-Shiga toxin (Stx) 2 humanized monoclonal antibody TMA-15 in mice lethally challenged with Stx-producing Escherichia coli J Infect Dis 184 738–42 Occurrence Handle10.1086/323082

    Article  Google Scholar 

  22. K Ikeda O Ida K Kimoto T Takatorige N Nakanishi K Tatara (1999) ArticleTitleEffect of early fosfomycin on treatment for prevention of hemolytic uremic syndrome accompanying Escherichia coli O157:H7 infection Clin Nephrol 52 357–62 Occurrence Handle10604643 Occurrence Handle1:CAS:528:DC%2BD3cXoslWlsg%3D%3D

    PubMed  CAS  Google Scholar 

  23. S Sawamura K Tanaka Y Koga (1999) ArticleTitleTherapeutic effects of anti-biotics against enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection: in vitro analysis using germ-free mice Kansenshogaku Zasshi 73 1054–63 Occurrence Handle10565122 Occurrence Handle1:STN:280:DC%2BD3c%2FjtVelsg%3D%3D

    PubMed  CAS  Google Scholar 

  24. H Hilpert H Brussow C Mietens J Sidoti L Lerner H Werchau (1987) ArticleTitleUse of bovine milk concentrate containing antibody to rotavirus to treat rotavirus gastroenteritis in infants J Infect Dis 156 158–66 Occurrence Handle3110303 Occurrence Handle1:STN:280:DyaL2s3lt1ersQ%3D%3D

    PubMed  CAS  Google Scholar 

  25. H Brussow H Hilpert I Walther J Sidoti C Mietens P Bachmann (1987) ArticleTitleBovine milk immunoglobulins for passive immunity to infantile rotavirus gastroenteritis J Clin Microbiol 25 982–6 Occurrence Handle3036910 Occurrence Handle1:STN:280:DyaL2s3ltFehuw%3D%3D

    PubMed  CAS  Google Scholar 

  26. T Ebina M Ohta M Kanamaru Y Yamamoto Y Osumi K Baba (1992) ArticleTitlePassive immunizations of suckling mice and infants with bovine colostrum containing antibodies to human rotavirus J Med Virol 38 117–23 Occurrence Handle1334126 Occurrence Handle1:STN:280:DyaK3s%2FpsVyhsA%3D%3D

    PubMed  CAS  Google Scholar 

  27. K Funatogawa T Ide F Kirikae K Saruta M Nakano T Kirikae (2002) ArticleTitleUse of immunoglobulin-enriched bovine colostrum against oral challenge with enterohaemorrhagic Escherichia coli O157:H7 in mice Microbiol Immunol 46 761–6 Occurrence Handle12516772 Occurrence Handle1:CAS:528:DC%2BD38XpvVSqtrs%3D

    PubMed  CAS  Google Scholar 

  28. R Lissner H Schmidt H Karch (1996) ArticleTitleA standard immunoglobulin preparation produced from bovine colostra shows antibody reactivity and neutralization activity against Shiga-like toxins and EHEC-hemolysin of Escherichia coli O157:H7 Infection 24 378–82 Occurrence Handle8923050 Occurrence Handle1:CAS:528:DyaK28XntlGrsLk%3D Occurrence Handle10.1007/BF01716084

    PubMed  CAS  Article  Google Scholar 

  29. M Noda T Yutsudo N Nakabayashi T Hirayama Y Takeda (1987) ArticleTitlePurification and some properties of Shiga-like toxin from Escherichia coli O157:H7 that is immunologically identical to Shiga toxin Microb Pathol 2 339–49 Occurrence Handle1:CAS:528:DyaL2sXksFertbw%3D Occurrence Handle10.1016/0882-4010(87)90076-3

    CAS  Article  Google Scholar 

  30. Iwakura N, Kojio S, Taneike I, Nakagawa S, Yamamoto T. The 77th Annual Meeting of Japanese Association for Infectious Diseases, Abstract 77, Fukuoka, 2003

  31. AW Paton J Paton (1998) ArticleTitlePathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections Clin Microbiol Rev 11 450–79 Occurrence Handle9665978 Occurrence Handle1:STN:280:DyaK1czjtlKlug%3D%3D

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Shizuo Yamamoto.

About this article

Cite this article

Kuribayashi, T., Seita, T., Fukuyama, M. et al. Neutralizing activity of bovine colostral antibody against verotoxin derived from enterohemorrhagic Escherichia coli O157:H7 in mice. J Infect Chemother 12, 251–256 (2006). https://doi.org/10.1007/s10156-006-0470-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10156-006-0470-y

Key words

  • Neutralization of verotoxin
  • Bovine colostral antibody
  • EHEC
  • Verotoxin
  • Hemolytic uremic syndrome
  • Mice