Skip to main content

Bactericidal activities of parenteral antibiotics and genotype of penicillin-binding protein in Streptococcus pneumoniae and Haemophilus influenzae isolated from children's blood

Abstract

A total of 16 isolates of Streptococcus pneumoniae and 18 isolates of Haemophilus influenzae were obtained from the blood of children admitted to the pediatric wards of hospitals in Hokkaido Kamikawa subprefecture between January 2003 and December 2005. The ages of the patients with S. pneumoniae or H. influenzae infection ranged from 2 months to 9 years and from 1 month to 4 years, respectively. The diagnoses of S. pneumoniae infection were as follows: pneumonia in 8 patients, occult bacteremia in 5 patients, and meningitis in 3 patients. The diagnoses of H. influenzae were: meningitis in 6 patients, pneumonia in 4 patients, occult bacteremia in 4 patients, epiglotitis in 2 patients, and facial cellulitis in 2 patients. Out of 16 S. pneumoniae isolates, penicillin-resistant strains with a mutation of 3 genes were observed in 7 children, and penicillin intermediate-resistant strains with a mutation of 1 or 2 genes were observed in 8 children. Out of 18 H. influenzae isolates, the β-lactamase-negative ampicillin-resistant strain with a substitution of 2 points in the ftsI gene was revealed in 2 children, the β-lactamase-negative ampicillin-resistant strain with a substitution of 1 point in the ftsI gene was observed in 4 children, the β-lactamase-positive amoxicillin/clavulanic acid-resistant strain with blaTEM-1 and ftsI with 2 substitutions in the ftsI gene was observed in 3 children, and the β-lactamase-positive ampicillin-resistant strain with blaTEM-1was not observed. The MBC90s of ampicillin, ceftriaxone, cefotaxime, meropenem, panipenem, and vancomycin against S. pneumoniae were 8 µg/ml, 1 µg/ml, 1 µg/ml 1 µg/ml, 0.25 µg/ml, and 0.5 µg/ml, respectively. Those of ampicillin, piperacillin, ceftriaxone, cefotaxime, meropenem, and panipenem against H. influenzae were >128 µg/ml, >128 µg/ml, 0.25 µg/mL, 1 µg/ml, 0.12 µg/ml, and 0.5 g/ml, respectively. It is suggest that the minimum bactricidal concentration (MBC) was dissociated from the minimum inhibitory concentration (MIC) in S. pneumoniae and H. influenzae with abnormal pbp genes.

This is a preview of subscription content, access via your institution.

References

  1. SD Brown MJ Rybak (2004) ArticleTitleAntimicrobial susceptibility of Streptococcus pneumoniae, Streptpcoccus pyogenes, and Haemophilus influenzae collected from patients across the USA, in 2001–2002, as part of the PROTEKT US study J Antimicrob Chemother 54 IssueIDSuppl A i7–15 Occurrence Handle15265831 Occurrence Handle1:CAS:528:DC%2BD2cXmtF2ls74%3D Occurrence Handle10.1093/jac/dkh313

    PubMed  CAS  Article  Google Scholar 

  2. GC Schito D Felmingham (2004) ArticleTitleSusceptibility of Streptococcus pneumoniae to penicillin, azithromycin and telithromycin (PROTEKT 1999–2003) Int J Antimicrob Agents 26 479–85 Occurrence Handle10.1016/j.ijantimicag.2005.04.022

    Article  Google Scholar 

  3. FC Fluit A Florijin J Verhoef D Milatovic (2005) ArticleTitleSusceptibility of European β-lactamase-positive and –negative Haemophilus influenzae isolates from the periods 1997/1998 and 2002/2003 J Antimicrob Chemother 56 133–8 Occurrence Handle15917287 Occurrence Handle1:CAS:528:DC%2BD2MXmsV2itLo%3D Occurrence Handle10.1093/jac/dki167

    PubMed  CAS  Article  Google Scholar 

  4. JA Karlowsky IA Critchley R Blosser-Middleton EA Karginova ME Jones C Thornsberry et al. (2002) ArticleTitleAntimicrobial surveillance of Haemophilus influenzae in the United States during 2000–2001 leads to detection of clonal dissemination of a β-lactamase-negative and ampicillin-resistant strains J Clin Microbiol 40 1063–6 Occurrence Handle11880440 Occurrence Handle1:CAS:528:DC%2BD38XisVKrsbo%3D Occurrence Handle10.1128/JCM.40.3.1063-1066.2002

    PubMed  CAS  Article  Google Scholar 

  5. K Ubukata N Chiba K Hasegawa R Kobayashi S Iwata K Sunakawa (2004) ArticleTitleAntibiotic susceptibility in relation to penicillin-binding protein genes and serotype distribution of Streptococcus pneumoniae strains responsible for meningitis in Japan, 1999 to 2002 Antimicrob Agents Chemother 48 1488–94 Occurrence Handle15105095 Occurrence Handle1:CAS:528:DC%2BD2cXjslClsrw%3D Occurrence Handle10.1128/AAC.48.5.1488-1494.2004

    PubMed  CAS  Article  Google Scholar 

  6. K Hasegawa N Chiba R Kobayashi SY Murayama S Iwata K Sunakawa et al. (2004) ArticleTitleRapidly increasing prevalence of β-lactamase-nonproducing, ampicillin-resistant Haemophilus influenzae type b in patients with meningitis Antimicrob Agents Chemother 48 1509–14 Occurrence Handle15105098 Occurrence Handle1:CAS:528:DC%2BD2cXjslClsrc%3D Occurrence Handle10.1128/AAC.48.5.1509-1514.2004

    PubMed  CAS  Article  Google Scholar 

  7. N Chiba R Kobayshi K Hasegawa M Morozumi E Nakayama T Tajima et al. (2005) ArticleTitleAntibiotic susceptibility according to genotype of penicillin-binding protein and macrolide resistance genes, and serotype of Streptococcus pneumoniae isolates from community-acquired pneumonia in children J Antimicrob Chemother 56 756–60 Occurrence Handle16131518 Occurrence Handle1:CAS:528:DC%2BD2MXhtVKiu7fP Occurrence Handle10.1093/jac/dki302

    PubMed  CAS  Article  Google Scholar 

  8. InstitutionalAuthorNameCenter for Disease Control and Prevention (1998) ArticleTitleProgress toward elimination of Haemophilus influenzae type b disease among infants and children: United States 1987–1997 MMWR 47 993–8

    Google Scholar 

  9. SL Kaplan Jr EO Mason ER Wald GE Shutze JS Gradley TQ Tan et al. (2004) ArticleTitleDecrease of invasive pneumococcal infections in children among 8 children's hospitals in the United States after the introduction of the 7-valent pneumococcal conjugate vaccine Pediatrics 113 443–9 Occurrence Handle14993532 Occurrence Handle10.1542/peds.113.3.443

    PubMed  Article  Google Scholar 

  10. National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial susceptibility testing: 12th informational supplement M100-S12. NCCLS, Wayne, PA, USA, 2002

  11. National Committee for Clinical Laboratory Standards. Methods for determining bactericidal activity of antimicrobial agents: approved guidelines. M26-A. NCCLS, Wayne, PA, USA, 1999

  12. Adams WG, Deaver KA, Cochi SL, Plikaytis BD, Zell ER, Broome CV, et al. Decline of childhood Haemophilus influenzae type b (Hib) disease in the Hib vaccine era. JAMA; 269:221–6

  13. MG Tauber O Zak WM Scheld B Hengstler MA Sande (1984) ArticleTitleThe postantibiotic effect in the treatment of experimental meningitis caused by Streptococcus pneumoniae in rabbits J Infect Dis 149 575–83 Occurrence Handle6609999 Occurrence Handle1:STN:280:BiuB3MvivVY%3D

    PubMed  CAS  Google Scholar 

  14. I Lutsar A Ahmed IR Friedland M Trujillo L Wubbel K Olsen et al. (1997) ArticleTitlePharmacodynamics and bactericidal activity of CTRX therapy in experimental cephalosporin-resistant pneumococcal meningitis Antimicrob Agents Chemother 41 2414–7 Occurrence Handle9371342 Occurrence Handle1:CAS:528:DyaK2sXntFKntb8%3D

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hiroshi Sakata.

About this article

Cite this article

Sakata, H. Bactericidal activities of parenteral antibiotics and genotype of penicillin-binding protein in Streptococcus pneumoniae and Haemophilus influenzae isolated from children's blood. J Infect Chemother 12, 338–342 (2006). https://doi.org/10.1007/s10156-006-0467-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10156-006-0467-6

Key words

  • Streptococcus pneumoniae
  • Haemophilus influenzae
  • Minimum inhibitory concentration (MIC)
  • Minimum bactricidal concentration (MBC)
  • Children