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Bacterial propagation in reusable water reservoirs in a humidified oxygen supply system


Bacterial colony counts in reusable water reservoirs in a humidified oxygen supply system maintained only by the addition of nonsterilized water, with or without washing (conventional procedure), were examined. Bacteria were detected in all the reservoirs even before the operation of the oxygen suppliers, and colony counts of more than 1 × 102/ml of bacteria were detected in some reservoirs without washing. Many bacteria were also detected in many reservoirs, after the operation of the oxygen suppliers, indicating that bacteria had contaminated the water reservoirs and propagated during this operation. These results suggest that it is not recommended to maintain these water reservoirs by the conventional procedure.

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  1. Centers for (1994) ArticleTitleDisease Control and Prevention. Guidelines for prevention of nosocomial pneumonia Respir Care 39 1191–236

    Google Scholar 

  2. NAM Cobben M Drent M Jonkers EFM Wouters M Vaneechoutte EE Stobberingh (1996) ArticleTitleOutbreak of severe Pseudomonas aeruginosa respiratory infections due to contaminated nebulizers J Hosp Infect 33 63–70 Occurrence Handle8738203 Occurrence Handle1:STN:280:BymA2MzntlE%3D Occurrence Handle10.1016/S0195-6701(96)90030-4

    PubMed  CAS  Article  Google Scholar 

  3. DE Craven DA Lichtenberg TA Goularte BJ Make WR McCabe (1984) ArticleTitleContaminated medication nebulizers in mechanical ventilator circuits Am J Med 77 834–8 Occurrence Handle6496537 Occurrence Handle1:STN:280:BiqD2czptVw%3D Occurrence Handle10.1016/0002-9343(84)90520-5

    PubMed  CAS  Article  Google Scholar 

  4. MS Favero LA Carson WW Bond NJ Petersen (1971) ArticleTitlePseudomonas aeruginosa: growth in distilled water from hospital Science 173 836–8 Occurrence Handle4999114 Occurrence Handle1:STN:280:CS6B1Mzit1I%3D

    PubMed  CAS  Google Scholar 

  5. TD Mastro BS Fields RF Breiman J Campbell BD Plikagtis JS Spina (1991) ArticleTitleNosocomial Legionnaires’ disease and use of medication nebulizers J Infect Dis 163 667–71 Occurrence Handle1995743 Occurrence Handle1:STN:280:By6C2c%2FgsVM%3D

    PubMed  CAS  Google Scholar 

  6. N Kobayashi T Yamazaki S Maesaki (2006) ArticleTitleBacteriological monitoring of water reservoirs in oxygen humidifiers: safety of prolonged and multipatient use of prefilled disposable oxygen humidifier bottles Infect Control Hosp Epidemiol 27 320–2 Occurrence Handle16532426 Occurrence Handle10.1086/503300

    PubMed  Article  Google Scholar 

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Correspondence to Tsutomu Yamazaki.

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Part of this work was presented at the 16th and 17th Annual Meetings of the Japanese Society of Environmental Infections, held in 2001 and 2002

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Kobayashi, N., Yamazaki, T., Oka, Y. et al. Bacterial propagation in reusable water reservoirs in a humidified oxygen supply system. J Infect Chemother 12, 160–162 (2006).

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Key words

  • Water reservoirs
  • Reuse
  • Bacterial contamination
  • Pseudomonadaceae
  • Respiratory infections