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Identification of the ETT2 locus in human diarrheagenic Escherichia coli by multiplex PCR


An Escherichia coli type III secretion system 2 (ETT2) locus was discovered in enterohemorrhagic E. coli O157:H7. To determine presence or absence of the ETT2 locus in diarrheagenic E. coli, a multiplex polymerase chain reaction (PCR) encoding Shiga toxins (stx1 and stx2), intimin (eaeA), and ETT2 (etrA) was developed for rapid detection. The ETT2 locus was identified not only in Shiga toxin-producing E. coli (STEC) but also in various non-STEC.

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Correspondence to Kayo Osawa.

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Osawa, K., Shibata, M., Nishiyama, Y. et al. Identification of the ETT2 locus in human diarrheagenic Escherichia coli by multiplex PCR. J Infect Chemother 12, 157–159 (2006).

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Key words

  • ETT2
  • Enterohemorrhagic Escherichia coli
  • Diarrheagenic Escherichia coli
  • Multiplex PCR