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Identification of the ETT2 locus in human diarrheagenic Escherichia coli by multiplex PCR

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Journal of Infection and Chemotherapy

Abstract

An Escherichia coli type III secretion system 2 (ETT2) locus was discovered in enterohemorrhagic E. coli O157:H7. To determine presence or absence of the ETT2 locus in diarrheagenic E. coli, a multiplex polymerase chain reaction (PCR) encoding Shiga toxins (stx1 and stx2), intimin (eaeA), and ETT2 (etrA) was developed for rapid detection. The ETT2 locus was identified not only in Shiga toxin-producing E. coli (STEC) but also in various non-STEC.

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Authors and Affiliations

  1. Department of Medical Technology, Faculty of Health Sciences, Kobe University School of Medicine, 7-10-2 Tomogaoka, Suma-ku, Kobe, 654-0142, Japan

    Kayo Osawa, Mariko Shibata, Yoko Nishiyama & Nobumasa Kataoka

  2. Department of Microbiology, Kobe Institute of Health, Kobe, Japan

    Manabu Kurokawa

  3. Department of Clinical Laboratory, Nishi-Kobe Medical Center, Kobe, Japan

    Go Yamamoto

  4. Department of Clinical Laboratory, Kobe University Hospital, Kobe, Japan

    Shohiro Kinoshita

Authors
  1. Kayo Osawa
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  2. Mariko Shibata
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  3. Yoko Nishiyama
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  4. Manabu Kurokawa
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  5. Go Yamamoto
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  6. Shohiro Kinoshita
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  7. Nobumasa Kataoka
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Corresponding author

Correspondence to Kayo Osawa.

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Osawa, K., Shibata, M., Nishiyama, Y. et al. Identification of the ETT2 locus in human diarrheagenic Escherichia coli by multiplex PCR. J Infect Chemother 12, 157–159 (2006). https://doi.org/10.1007/s10156-006-0435-1

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  • DOI: https://doi.org/10.1007/s10156-006-0435-1

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