Skip to main content

Advertisement

Log in

Emergence of rifampicin resistance in methicillin-resistant Staphylococcus aureus in tuberculosis wards

  • NOTE
  • Published:
Journal of Infection and Chemotherapy

Abstract

To assess whether the occurrence of rifampicin (RFP) resistance in methicillin-resistant Staphylococcus aureus (MRSA) is related to treatment of tuberculosis, we determined the RFP susceptibility of MRSA isolates obtained from tuberculosis patients and screened for mutation(s) in the rpoB gene of these isolates. The MICs of RFP for 84 MRSA isolates obtained from two hospitals in Japan were determined. DNA was sequenced in the region 1318–1602 nucleotides (nt) of the rpoB gene, which includes RFP resistance-determining clusters I (1384–1464 nt, 462–488 amino acids). The majority of MRSA isolates from tuberculosis wards, i.e., 48 of 51 (94%) [33 of 34 in a Tokyo hospital (97%) and 15 of 17 in a Chubu hospital (88%)], were resistant to RFP. Meanwhile, no isolates of 33 from the other wards were resistant to RFP. All RFP-resistant MRSA isolates had a mutation(s), including novel mutation(s) such as Val453ÆPhe, Asp471ÆAsn, and Ile527ÆLeu, in rpoB. An emergence of RFP-resistant MRSAs in tuberculosis wards in Japan was strongly suggested.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. ES Darley AP MacGowan (2004) ArticleTitleAntibiotic treatment of gram-positive bone and joint infections J Antimicrob Chemother 53 928–35 Occurrence Handle10.1093/jac/dkh191 Occurrence Handle1:CAS:528:DC%2BD2cXksVait7Y%3D Occurrence Handle15117932

    Article  CAS  PubMed  Google Scholar 

  2. FJ Schmitz ME Jones (1997) ArticleTitleAntibiotics for treatment of infections caused by MRSA and elimination of MRSA carriage. What are the choices? Int J Antimicrob Agents 9 1–19 Occurrence Handle1:CAS:528:DyaK2sXmtV2itbk%3D Occurrence Handle10.1016/S0924-8579(97)00027-7

    Article  CAS  Google Scholar 

  3. W Wehrli (1983) ArticleTitleRifampin: mechanisms of action and resistance Rev Infect Dis 5 S407–11 Occurrence Handle1:CAS:528:DyaL3sXlslantrs%3D Occurrence Handle6356275

    CAS  PubMed  Google Scholar 

  4. H Aubry-Damon CJ Soussy P Courvalin (1998) ArticleTitleCharacterization of mutations in the rpoB gene that confer rifampin resistance in Staphylococcus aureus Antimicrob Agents Chemother 42 2590–4 Occurrence Handle1:CAS:528:DyaK1cXms1Cqu7Y%3D Occurrence Handle9756760

    CAS  PubMed  Google Scholar 

  5. TA Wichelhaus V Schäfer V Brade B Böddinghaus (1999) ArticleTitleMolecular characterization of rpoB mutations conferring cross-resistance to rifamycins on methicillin-resistant Staphylococcus aureus Antimicrob Agents Chemother 43 2813–6 Occurrence Handle1:CAS:528:DyaK1MXnt1Wrurg%3D Occurrence Handle10543773

    CAS  PubMed  Google Scholar 

  6. TA Wichelhaus V Schäfer V Brade B Böddinghaus (2001) ArticleTitleDifferential effect of rpoB mutations on antibacterial activities of rifampicin and KRM-1648 against Staphylococcus aureus J Antimicrob Chemother 47 153–6 Occurrence Handle10.1093/jac/47.2.153 Occurrence Handle1:CAS:528:DC%2BD3MXhtlGgurk%3D Occurrence Handle11157898

    Article  CAS  PubMed  Google Scholar 

  7. TA Wichelhaus B Boddinghaus S Besier V Schafer V Brade A Ludwig (2002) ArticleTitleBiological cost of rifampin resistance from the perspective of Staphylococcus aureus Antimicrob Agents Chemother 46 3381–5 Occurrence Handle10.1128/AAC.46.11.3381-3385.2002 Occurrence Handle1:CAS:528:DC%2BD38Xot1ykt7k%3D Occurrence Handle12384339

    Article  CAS  PubMed  Google Scholar 

  8. T Fujino J Sekiguchi A Kawana H Konosaki H Nishimura K Saruta et al. (2004) ArticleTitleMolecular epidemiology of methicillin-resistant Staphylococcus aureus in a Tokyo hospital in 2003 Jpn J Infect Dis 57 83–5 Occurrence Handle1:CAS:528:DC%2BD2cXlsFGjtbc%3D Occurrence Handle15118219

    CAS  PubMed  Google Scholar 

  9. T Fujino J Sekiguchi A Kawana H Konosaki H Nishimura K Saruta et al. (2002) ArticleTitleMolecular epidemiology of methicillin-resistant Staphylococcus aureus in a Tokyo hospital in 2002 Jpn J Infect Dis 55 210–3 Occurrence Handle1:CAS:528:DC%2BD3sXjtVagurs%3D Occurrence Handle12606833

    CAS  PubMed  Google Scholar 

  10. T Fujino N Mori A Kawana Y Naiki H Kawahata T Kuratsuji K Kudo et al. (2001) ArticleTitleMolecular epidemiology of methicillin-resistant Staphylococcus aureus in a Tokyo hospital in 2001 Jpn J Infect Dis 54 240–2 Occurrence Handle1:CAS:528:DC%2BD38Xjtlyrs7s%3D Occurrence Handle11862007

    CAS  PubMed  Google Scholar 

  11. T Fujino N Mori A Kawana H Kawabata T Kuratsuji K Kudo et al. (2001) ArticleTitleMolecular epidemiology of methicillin-resistant Staphylococcus aureus in a Tokyo hospital in 2000 Jpn J Infect Dis 54 91–3 Occurrence Handle1:STN:280:DC%2BD3Mzms1Ortg%3D%3D Occurrence Handle11427757

    CAS  PubMed  Google Scholar 

  12. N Mori H Kawata T Hama T Fujino H Kawahata A Kawana et al. (2001) ArticleTitleAn outbreak of methicillin-resistant Staphylococcus aureus (MRSA) in a tuberculosis ward Jpn J Infect Dis 54 157–9 Occurrence Handle1:STN:280:DC%2BD3MnhtVGltQ%3D%3D Occurrence Handle11684789

    CAS  PubMed  Google Scholar 

  13. M Hosoda J Toyama K Hasada T Fujino R Ohtsuki T Kirikae (2002) ArticleTitleA clonal expansion of methicillin-resistant Staphylococcus aureus (MRSA) in a tuberculosis ward Jpn J Infect Dis 55 19–21

    Google Scholar 

  14. InstitutionalAuthorNameNational Committee for Clinical Laboratory Standards (2003) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 6th ed. M7-A5: 2003 NCCLS Wayne, PA

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Teruo Kirikae.

About this article

Cite this article

Sekiguchi, Ji., Fujino, T., Araake, M. et al. Emergence of rifampicin resistance in methicillin-resistant Staphylococcus aureus in tuberculosis wards. J Infect Chemother 12, 47–50 (2006). https://doi.org/10.1007/s10156-005-0417-8

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10156-005-0417-8

Key words

Navigation