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In vitro activity of daptomycin against clinical isolates ofGram-positive bacteria


We determined the activity of daptomycin, a recently FDA-approved antimicrobial agent, against clinical isolates of Gram-positive bacteria, including viridans group streptococci (16 Streptococcus mitis species group, 12 S. mutans species group, 9 S. anginosus species group, 8 S. sanguinis species group, 5 S. salivarius species group) from patients with infective endocarditis, 32 methicillin-resistant Staphylococcus aureus, 32 high-level penicillin-resistant Streptococcus pneumoniae, 38 vancomycin-resistant enterococci (including 1 linezolid-resistant isolate), and thefollowing unusual Gram-positive bacteria: 3 Listeria monocytogenes, 4 Erysipelothrix rhusiopathiae, 9 Corynebacterium species, 10 Abiotrophia/Granulicatella species, 2 Rothia (Stomatococcus) mucilaginosus, and 4 Gemella morbillorum. Daptomycin minimum inhibitory concentration (MIC)90 values for the viridans group streptococci, methicillin-resistant S. aureus, penicillin-resistant S. pneumoniae, and Enterococcus species were 0.5, 0.5, ≤0.125, and 4 µg/ml, respectively. The daptomycin MIC range for the unusual Gram-postitive bacteria was ≤0.125–2 µg/ml. We conclude that daptomycin has in vitro activity against viridans group streptococci associated with endocarditis as well as against several types of unusual Gram-positive bacteria that can cause endocarditis.

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Correspondence to Robin Patel.

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Presented in part at the 13th European Congress of Clinical Microbiology and Infectious Diseases, Glasgow, 2003 and at the 44th ICAAC, Washington, DC, 2004

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Piper, K., Steckelberg, J. & Patel, R. In vitro activity of daptomycin against clinical isolates ofGram-positive bacteria. J Infect Chemother 11, 207–209 (2005).

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Key words

  • Daptomycin
  • Gram-positive bacteria
  • Antimicrobial resistance
  • Endocarditis
  • Enterococci
  • Streptococci