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A rapid antimicrobial susceptibility test based on chemiluminescence assay and its application to screening of genotypes in vancomycin-resistant enterococci

Abstract

Minimum inhibitory concentrations (MICs) of vancomycin (VCM) and teicoplanin (TEIC) were measured using a novel susceptibility test based on the chemiluminescence assay (CA) method (Rapid-Lumi Eiken; Eiken Chemicals, Tokyo, Japan) against 33 strains in total: 7, 5, and 10 strains of which are VCM-resistant enterococci (VRE) with vanA, vanB, and vanC genes, respectively, and the other 11 strains are vancomycin-susceptible enterococci (VSE). The results were in good accordance with the values determined by the standard broth dilution method approved by the National Committee for Clinical Laboratory Standards (NCCLS): i.e., 88% (29/33) of consistency for VCM and 97% (32/33) for TEIC, respectively. In addition, genotypes in VRE strains (vanA, vanB, vanC-1, and vanC-2/3 genes) were properly estimated from the results of the CA method and the NCCLS interpretive categories, even though the incubation time was very short (2–4 h). In conclusion, it was found that the new method is reliable and rapid to detect VRE strains in clinical laboratories.

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Correspondence to Zenzo Nagasawa.

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Nagasawa, Z., Manome, I. & Nagayama, A. A rapid antimicrobial susceptibility test based on chemiluminescence assay and its application to screening of genotypes in vancomycin-resistant enterococci. J Infect Chemother 10, 220–226 (2004). https://doi.org/10.1007/s10156-004-0329-z

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  • DOI: https://doi.org/10.1007/s10156-004-0329-z

Key words

  • Vancomycin-resistant enterococci
  • Rapid antimicrobial susceptibility test
  • Chemiluminescence assay