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A comparative evaluation of phenotypic and molecular methods for the detection of oxacillin resistance in coagulase-negative staphylococci

Abstract

Detection of oxacillin resistance in coagulase-negative staphylococci (C-NS) by phenotypic methods is often difficult. The present study compared the National Committee for Clinical Laboratory Standards (NCCLS) revised guidelines of phenotypic methods with a mecA-based polymerase chain reaction (PCR) for C-NS. Ninety clinical C-NS isolates were tested for oxacillin resistance by disk diffusion (1-µg disk), minimum inhibitory concentration (MIC) breakpoint (0.5 µg/ml) after 24 h, and mecA-based PCR. The sensitivity and specificity of disk diffusion was 80% and 93%, and the sensitivity and specificity of the MIC breakpoint after 24 h was 84% and 91%, respectively, against PCR as gold standard. Eleven strains (7 mecA-positive and 4 mecA-negative) showed discordant results between MIC breakpoint after 24 h and PCR. Six of the 7 mecA-positive and all 4 mecA-negative discordant strains had inducible oxacillin resistance and Β-lactamase hyperproduction, respectively. The present study concludes that inducible oxacillin resistance and Β-lactamase hyperproduction are the major causes of discordant results between phenotypic methods and mecA-based PCR, and need special attention.

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Correspondence to Kashi N. Prasad.

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Ghoshal, U., Prasad, K., Singh, M. et al. A comparative evaluation of phenotypic and molecular methods for the detection of oxacillin resistance in coagulase-negative staphylococci. J Infect Chemother 10, 86–89 (2004). https://doi.org/10.1007/s10156-003-0293-z

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  • DOI: https://doi.org/10.1007/s10156-003-0293-z

Key words

  • Β-lactamase
  • MecA
  • Methicillin resistance
  • PCR