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In-vitro activity of sitafloxacin (DU-6859a), either singly or in combination with rifampin analogs, against Mycobacterium leprae

Abstract

 The in-vitro antibacterial activity of sitafloxacin (DU-6859a) against Mycobacterium leprae was evaluated and compared with those of ofloxacin, levofloxacin, and ciprofloxacin. Two biochemical indicators (intracellular ATP and uptake of [3H]-thymidine) were used to measure the in-vitro growth of M. leprae in Dhople-Hanks (DH) medium. Sitafloxacin was found to be more potent than the other three commonly used fluoroquinolones, with the minimum inhibitory concentration (MIC) against M. leprae being 0.1875 μg/ml and the action being bactericidal. The MICs of ofloxacin, levofloxacin, and ciprofloxacin were 1.5, 0.75, and 3.0 μg/ml, respectively. Similar to ofloxacin and levofloxacin, sitafloxacin also exhibited synergistic activity when combined with either rifabutin or KRM-1648, but not with rifampin. Thus, further studies on the incorporation of sitafloxacin in multidrug therapy regimens in treating leprosy patients are suggested.

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Received: July 30, 2002 / Accepted: October 9, 2002

Acknowledgments The authors wish to thank Daiichi Pharmaceutical Company, Ltd. for supplying sitafloxacin for this study.

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Dhople, A., Namba, K. In-vitro activity of sitafloxacin (DU-6859a), either singly or in combination with rifampin analogs, against Mycobacterium leprae . J Infect Chemother 9, 12–15 (2003). https://doi.org/10.1007/s10156-002-0212-8

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  • DOI: https://doi.org/10.1007/s10156-002-0212-8

  • Key words Sitafloxacin
  • M. leprae
  • ATP
  • 3H-thymidine
  • Rifamycins
  • Fluoroquinolones