Abstract
Objective
Dysregulated circular RNAs (circRNAs) have been verified to function in the development of gastric cancer (GC). The current study was designed to investigate the role of circ_0000419 in GC progression, and the potential mechanistic pathway.
Methods
Relative expression of circ_0000419, microRNA-300 (miR-300) and Repulsive Guidance Molecule B (RGMB) was analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay. Cell metastasis, including migration and invasion, was assessed by wound healing and Transwell assays. Glucose consumption and lactate production were examined using kits. The association between miR-300 and circ_0000419 or RGMB was validated by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assays. Role of circ_0000419 in vivo was determined by xenograft experiment.
Results
Circ_0000419 and RGMB were downregulated, while miR-300 was upregulated in GC tissues and cells. Gain of circ_0000419 inhibited migration, invasion and glycolysis in GC cells, which was attenuated by introduction of miR-300 or silencing of RGMB. Circ_0000419 sponged miR-300, and RGMB was direct target of miR-300. Circ_0000419 overexpression could block GC tumor growth in vivo.
Conclusion
Circ_0000419 inhibited GC cell migration, invasion and glycolysis through regulation of miR-300/RGMB axis, at least in part, affording a molecular target for GC treatment.
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Data availability
Not applicable.
Change history
13 September 2023
A Correction to this paper has been published: https://doi.org/10.1007/s10147-023-02409-8
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Funding
Jiaxing City Science and Technology Plan Project (No.2019AD32262). 2019 Jiaxing Key Discipline of Medicine–Oncology (Supporting Subject) 2019-zc-11. 2023 Jiaxing city and provinces to build medical key disciplines-Oncology (2023-SSGJ-001).
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JL designed and performed the research; YZ and ZC analyzed the data; HJ, JL and GD wrote the manuscript. All authors read and approved the final manuscript.
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Written informed consents were obtained from all participants and this study was permitted by the Ethics Committee of The First Hospital of Jiaxing, The Affiliated Hospital of Jiaxing University.
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Lu, J., Zhou, Y., Chen, Z. et al. Circ_0000419 acts as a tumor suppressor in gastric cancer development via regulating miR-300/RGMB axis. Int J Clin Oncol 28, 1475–1485 (2023). https://doi.org/10.1007/s10147-023-02379-x
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DOI: https://doi.org/10.1007/s10147-023-02379-x