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Diagnostic performance of fluorescence in situ hybridization (FISH) in upper tract urothelial carcinoma (UTUC): a systematic review and meta-analysis

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Abstract

Background

Fluorescence in situ hybridization (FISH) is a technique that uses fluorescently labeled DNA probes. Many studies have evaluated the ROC curve (sensitivity and specificity) with the FISH method to diagnose upper tract urothelial carcinoma (UTUC). The current meta-analysis was performed to examine the diagnostic power of the FISH method in UTUC.

Methods

We reviewed databases and methodically obtained papers for analysis until April 25th, 2022. The Meta-disc V.1.4 and Comprehensive Meta-Analysis V.3.3 software calculated the pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio, area under the curve (AUC), and summary receiver-operating characteristic (SROC). The I2 and Chi-square tests were used to examine the heterogeneity. Finally, the publication bias was estimated using Begg’s and Egger’s tests.

Results

A total of 13 articles included 1,067 participants (439 cases and 628 controls). The overall pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the curve (AUC) were 0.72 (95% CI 0.67–0.76), 0.95 (95% CI 0.93–0.97), 10.42 (95% CI 5.84–18.60), 0.29 (95% CI 0.21–0.40), 38.55 (95% CI 18.58–79.96), and 0.91, respectively. No publication bias was reported based on Begg’s and Egger’s tests (Begg’s p = 0.200; Egger’s p = 0.151).

Conclusion

This paper clearly shows that the high specificity and acceptable sensitivity of the FISH method make it a promising diagnostic method for UTUC in urine samples. However, further research with higher statistical numbers is needed to strengthen the correlation and be used for diagnostic applications.

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Data availability

Data associated with this study can be accessed form the corresponding author upon a reasonable request.

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Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Biology, Mashhad Branch, Islamic Azad University, 9187147578, Mashhad, Iran

    Amir Hossein Aalami

  2. Student Research Committee, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran

    Farnoosh Aalami

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  1. Amir Hossein Aalami
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Contributions

Conceptualization, AHA.; searching and resources, AHA. and FA.; writing—original draft preparation, AHA.; writing—review and editing, FA.; supervision, AHA All the authors have read and agreed to the published version of the manuscript. All the named authors meet the International Committee of Medical Journal Editors (ICMJE) criteria for authorship for this article, take responsibility for the integrity of the work as a whole, and have given their approval for this version to be published.

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Correspondence to Amir Hossein Aalami.

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Aalami, A.H., Aalami, F. Diagnostic performance of fluorescence in situ hybridization (FISH) in upper tract urothelial carcinoma (UTUC): a systematic review and meta-analysis. Int J Clin Oncol 27, 1605–1615 (2022). https://doi.org/10.1007/s10147-022-02216-7

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