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Genetic association of promoter in GRP78 gene with nasopharyngeal carcinoma in a Chinese population

International Journal of Clinical Oncology Aims and scope Submit manuscript

Abstract

Background

Emerging evidences were accumulated to support the view that GRP78 might be associated with multiple types of cancer. Given these, the aim of this study is to investigate the relationship between single nucleotide polymorphisms (SNPs) of GRP78 gene promoter and nasopharyngeal carcinoma (NPC).

Methods

Three SNPs (rs3216733, rs17840761 and rs17840762) in GRR78 promoter were estimated in 422 NPC patients and 452 controls. Genotyping was performed using SNaPshot SNP. Serum GRP78 level was performed by enzyme-linked immunosorbent assay (ELISA). Data were analyzed by SPSS 17.0 software.

Results

Significant association between rs3216733 polymorphism and NPC was observed (Cd vs. dd: OR = 0.57, 95% CI 0.43–0.76, P < 0.001; CC vs. dd: OR = 0.62, 95% CI 0.39–0.98, P = 0.043; Cd/CC vs. dd: OR = 0.58, 95% CI 0.44–0.76, P < 0.001; C vs. d OR = 0.70, 95% CI 0.57–0.86, P = 0.001). Additionally, we further found that expression were down-regulated in serum of patients with NPC carrying rs3216733 CC genotype when compared to that of dd genotype (P < 0.001).

Conclusion

The observations suggest that rs3216733 polymorphism in the GRP78 gene promoter may correlate with NPC susceptibility.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (81560461). This study was supported by medical high-level talent training plan and thousands of young and middle-aged backbone teachers cultivation plan of Guangxi province and the 2018–2020 professional and experimental practice teaching base construction projects of Guangxi province. The authors acknowledge all the participants involved in this study for their support.

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Correspondence to Jun-Li Wang.

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Wang, R., Qin, HM., Qin, L. et al. Genetic association of promoter in GRP78 gene with nasopharyngeal carcinoma in a Chinese population. Int J Clin Oncol 24, 359–365 (2019). https://doi.org/10.1007/s10147-018-1366-4

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  • DOI: https://doi.org/10.1007/s10147-018-1366-4

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