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Identification of patients with nasopharyngeal carcinoma by serum protein profiling using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry

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Abstract

Background

As diagnosis of nasopharyngeal carcinoma at an early disease stage is important, we attempted to distinguish between patients with nasopharyngeal carcinoma and noncancer controls by using serum protein profiles.

Methods

Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry and CM10 protein chip were used to detect the serum proteomic patterns of 65 patients with nasopharyngeal carcinoma before radiotherapy and 93 noncancer controls. Proteomic spectra of serum samples from 50 nasopharyngeal carcinoma patients and 60 noncancer controls were used as a training set. The validity of the classification tree was then challenged with a blind test set which included another 15 patients with nasopharyngeal carcinoma and 33 noncancer controls. Biomarker Wizard 3.01 and Biomarker Pattern 5.01 were used in combination to analyze the data and to develop diagnostic models.

Results

21 protein peaks were significantly different between nasopharyngeal carcinoma and controls. 4 mass peaks (M4182, M5343, M5913 and M8702 mass/charge ratio) were chosen automatically to construct a classification tree. The classification tree correctly determined 93.8 % (45/48) of the test samples with 93.3 % (14/15) of the nasopharyngeal carcinoma samples and 93.9 % (31/33) of the noncancer samples. Using a combination of serum protein profiles and Epstein−Barr viral capsid antigen immunoglobulin A antibody tests, the diagnostic sensitivity and specificity were increased to 100 and 97 %, respectively.

Conclusions

Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry could correctly distinguish nasopharyngeal carcinoma from noncancer individuals and showed great potential for the development of a screening test for the detection of nasopharyngeal carcinoma.

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Acknowledgments

The work was sponsored by the clinical research special foundation of Wu Jieping Medical Foundation (No.320.6750.06080), Guangxi Scientific Research and Technology Development Project (No.0719006-2-23), Guangxi Graduate Education and Innovation Project (No.2007105981002 M33), supplementary programs of Guangxi large instruments shared network test (No.533-2007-112) (No. 555-2007-134), major research projects of Guangxi universities (No. 201101ZD004), and the key project of Guangxi health care (No. 2011076). Xiao-dong Zhu and Fang Su contributed equally to this article. All authors read and approved the final manuscript.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Radiotherapy, Guangxi Autonomous Regional Cancer Hospital, Cancer Hospital of Guangxi Medical University, Nanning, 530021, China

    Xiao-dong Zhu, Fang Su, Zhong-guo Liang, Ling Li, Song Qu, Xia Liang, Shi-xiong Liang & Long Chen

  2. Department of the Sixth Laboratory, Guangxi Autonomous Regional Cancer Hospital, Cancer Hospital of Guangxi Medical University, Nanning, 530021, China

    Qi Wang

Authors
  1. Xiao-dong Zhu
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  2. Fang Su
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  3. Zhong-guo Liang
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  4. Ling Li
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  5. Song Qu
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  6. Xia Liang
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  7. Qi Wang
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  8. Shi-xiong Liang
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  9. Long Chen
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Corresponding author

Correspondence to Xiao-dong Zhu.

Additional information

X. Zhu and F. Su were co-first authors.

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Zhu, Xd., Su, F., Liang, Zg. et al. Identification of patients with nasopharyngeal carcinoma by serum protein profiling using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. Int J Clin Oncol 19, 579–585 (2014). https://doi.org/10.1007/s10147-013-0621-y

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  • DOI: https://doi.org/10.1007/s10147-013-0621-y

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