The pathogenesis shared between abdominal aortic aneurysms and intracranial aneurysms: a microarray analysis

  • Wen Wang
  • Hao Li
  • Zheng Zhao
  • Haoyuan Wang
  • Dong Zhang
  • Yan Zhang
  • Qing Lan
  • Jiangfei Wang
  • Yong Cao
  • Jizong Zhao
Original Article
  • 73 Downloads

Abstract

Abdominal aortic aneurysms (AAAs) and intracranial saccular aneurysms (IAs) are the most common types of aneurysms. This study was to investigate the common pathogenesis shared between these two kinds of aneurysms. We collected 12 IAs samples and 12 control arteries from the Beijing Tiantan Hospital and performed microarray analysis. In addition, we utilized the microarray datasets of IAs and AAAs from the Gene Expression Omnibus (GEO), in combination with our microarray results, to generate messenger RNA expression profiles for both AAAs and IAs in our study. Functional exploration and protein–protein interaction (PPI) analysis were performed. A total of 727 common genes were differentially expressed (404 was upregulated; 323 was downregulated) for both AAAs and IAs. The GO and pathway analyses showed that the common dysregulated genes were mainly enriched in vascular smooth muscle contraction, muscle contraction, immune response, defense response, cell activation, IL-6 signaling and chemokine signaling pathways, etc. The further protein–protein analysis identified 35 hub nodes, including TNF, IL6, MAPK13, and CCL5. These hub node genes were enriched in inflammatory response, positive regulation of IL-6 production, chemokine signaling pathway, and T/B cell receptor signaling pathway. Our study will gain new insight into the molecular mechanisms for the pathogenesis of both types of aneurysms and provide new therapeutic targets for the patients harboring AAAs and IAs.

Keywords

Abdominal aortic aneurysms Intracranial aneurysms Microarray Gene ontology Pathway analysis 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interest.

Ethical statement

This study was approved by the Ethics Committee of the Department of Medicine, Beijing Tiantan Hospital, Capital Medical University (KY2011-002-02) and the national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants who were included in the study.

Supplementary material

10143_2017_912_MOESM1_ESM.xlsx (12 kb)
Supplementary Table 1 Identification of top 30 canonical pathways using IPA. (XLSX 11 kb)
10143_2017_912_MOESM2_ESM.docx (465 kb)
Supplementary Table 2 Genes connectivity in Protein-Protein Interaction network. (DOCX 465 kb)

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Department of Neurosurgery, Beijing Tiantan HospitalCapital Medical UniversityBeijingChina
  2. 2.Department of NeurosurgeryThe Second Affiliated Hospital of Soochow UniversitySuzhouChina
  3. 3.China National Clinical Research Center for Neurological DiseasesBeijingChina
  4. 4.Beijing Key Laboratory of Translational Medicine for Cerebrovascular DiseasesBeijingChina
  5. 5.Beijing Neurosurgical InstituteCapital Medical UniversityBeijingChina
  6. 6.Department of NeurosurgeryThe First Affiliated Hospital of Anhui Medical UniversityHefeiChina

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