The pathogenesis shared between abdominal aortic aneurysms and intracranial aneurysms: a microarray analysis
Abdominal aortic aneurysms (AAAs) and intracranial saccular aneurysms (IAs) are the most common types of aneurysms. This study was to investigate the common pathogenesis shared between these two kinds of aneurysms. We collected 12 IAs samples and 12 control arteries from the Beijing Tiantan Hospital and performed microarray analysis. In addition, we utilized the microarray datasets of IAs and AAAs from the Gene Expression Omnibus (GEO), in combination with our microarray results, to generate messenger RNA expression profiles for both AAAs and IAs in our study. Functional exploration and protein–protein interaction (PPI) analysis were performed. A total of 727 common genes were differentially expressed (404 was upregulated; 323 was downregulated) for both AAAs and IAs. The GO and pathway analyses showed that the common dysregulated genes were mainly enriched in vascular smooth muscle contraction, muscle contraction, immune response, defense response, cell activation, IL-6 signaling and chemokine signaling pathways, etc. The further protein–protein analysis identified 35 hub nodes, including TNF, IL6, MAPK13, and CCL5. These hub node genes were enriched in inflammatory response, positive regulation of IL-6 production, chemokine signaling pathway, and T/B cell receptor signaling pathway. Our study will gain new insight into the molecular mechanisms for the pathogenesis of both types of aneurysms and provide new therapeutic targets for the patients harboring AAAs and IAs.
KeywordsAbdominal aortic aneurysms Intracranial aneurysms Microarray Gene ontology Pathway analysis
This research was supported by the following funds: Cooperative project between The Science and Technology Ministry of China and Canada titled “Research on the genetics and pathogenesis of intracranial aneurysm and arteriovenous malformation,” led by Professor Jizong Zhao (No. 2010dfb30850); Beijing Municipal Administration of Hospitals’ Mission Plan (SML20150501); “11th Five-Year Plan” the National Science and Technology supporting plan (2006BAI01A13); “13th Five-Year Plan” National Science and Technology supporting plan (2015BAI09B04); the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2013BAI09B03); and “973” the Ministry of Science and Technology of China (2012CB825505).
Compliance with ethical standards
Conflict of interest
The authors declare that there is no conflict of interest.
This study was approved by the Ethics Committee of the Department of Medicine, Beijing Tiantan Hospital, Capital Medical University (KY2011-002-02) and the national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.
Informed consent was obtained from all individual participants who were included in the study.
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