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Decompressive craniectomy and mild hypothermia reduces infarction size and counterregulates Bax and Bcl-2 expression after permanent focal ischemia in rats

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Abstract

Both mild hypothermia (MH) and decompressive craniectomy (CE) have been shown to have neuroprotective effects in brain ischemia. We investigated a possible effect of MH and a combination of CE and MH (CE+MH) on the changes of infarction size, DNA fragmentation, and immunoreactivities for Bcl-2 and Bax after 24 h of permanent middle cerebral artery occlusion (MCAO) in rats. For the estimation of ischemic brain injury, we calculated the infarct size of the MCA region at 24 h after the MCAO. Terminal deoxynucleotidyl transferase-mediated dUTP–biotin in situ nick labeling (TUNEL) staining was performed for the detection of DNA fragmentation. Immunoreactivities for Bcl-2 and Bax were stained. Infarction size after permanent MCAO was significantly reduced by CE+MH treatment (P<0.01). Infarction size did not change significantly by application of MH alone (P>0.05). TUNEL staining was remarkably reduced both in MH-treated animals and in CE+MH-treated animals. Immunoreactivity for Bcl-2 was greatly induced both in MH-treated animals and in CE+MH-treated animals. Induction of immunoreactivity for Bcl-2 was obviously inhibited both in MH-treated animals and in CE+MH-treated animals. It suggests that temporary MH delays infarct evolution and ameliorates neuron apoptosis but does not significantly reduce definite infarction size. CE+MH not only ameliorates neuron apoptosis but also remarkably reduces infarction size.

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Acknowledgement

This study was supported in part by a Japan–China Sasakawa Medical Fellowship.

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Authors and Affiliations

  1. Neurosurgery Department, The First Affliated Hospital to Suzhou University, Suzhou, People’s Republic of China

    Bian Jieyong, Wang Zhong, Zhang Shiming & Zhou Dai

  2. Neurosurgery Department, Fujita Health University, Toyoake, 470-1192, Aichi, Japan

    Yoko Kato, Tetsuo Kanno & Hirotoshi Sano

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  1. Bian Jieyong
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  2. Wang Zhong
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  3. Zhang Shiming
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  4. Zhou Dai
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  5. Yoko Kato
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  7. Hirotoshi Sano
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Correspondence to Bian Jieyong.

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Jieyong, B., Zhong, W., Shiming, Z. et al. Decompressive craniectomy and mild hypothermia reduces infarction size and counterregulates Bax and Bcl-2 expression after permanent focal ischemia in rats. Neurosurg Rev 29, 168–172 (2006). https://doi.org/10.1007/s10143-005-0010-8

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  • DOI: https://doi.org/10.1007/s10143-005-0010-8

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