Abstract
Hepatocellular carcinoma (HCC) is a leading cause of cancer-related mortality globally. Many herbal medicines and their bioactive compounds have shown anti-tumor properties. This study was conducted to examine the effect of psilostachyin C (PSC), a sesquiterpenoid lactone isolated from Artemisia vulgaris L., in the malignant properties of HCC cells. CCK-8, flow cytometry, wound healing, and Transwell assays revealed that 25 μM PSC treatment significantly suppressed proliferation, cell cycle progression, migration, and invasion of two HCC cell lines (Hep 3B and Huh7) while promoting cell apoptosis. Bioinformatics prediction suggests CREB binding protein (CREBBP) as a promising target of PSC. CREBBP activated transcription of GATA zinc finger domain containing 2B (GATAD2B) by binding to its promoter. CREBBP and GATAD2B were highly expressed in clinical HCC tissues and the acquired HCC cell lines, but their expression was reduced by PSC. Either upregulation of CREBBP or GATAD2B restored the malignant properties of HCC cells blocked by PSC. Collectively, this evidence demonstrates that PSC pocessess anti-tumor functions in HCC cells by blocking CREBBP-mediated transcription of GATAD2B.
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Acknowledgements
We thanks to the Key research and development project of Shaanxi Province (Approval no. 2021SF-181) for the funding support.
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This study was supported by the Key research and development project of Shaanxi Province (Approval no. 2021SF-181).
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KJ conceived the study and conducted the experiments; NN is responsible for data collection, analyzed and interpreted the data; JH wrote the manuscript and revised the manuscript and important intellectual content. YC performed experiments and collected data; RW analyzed the results and reviewed the manuscript; JM performed experiments and statistical analysis. All authors read and approved the final manuscript.
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Jiang, K., Ning, N., Huang, J. et al. Psilostachyin C reduces malignant properties of hepatocellular carcinoma cells by blocking CREBBP-mediated transcription of GATAD2B. Funct Integr Genomics 24, 75 (2024). https://doi.org/10.1007/s10142-024-01353-8
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DOI: https://doi.org/10.1007/s10142-024-01353-8