Abstract
In recent years, the potassium voltage-gated channel subfamily D (KCND) channels, particularly KCND2 (also known as Kv4.2), have been suggested to play a role in a variety of cancers, but their role in breast cancer has not yet been revealed. We analyzed RNA sequencing data from The Cancer Genome Atlas database and the Genotype-Tissue Expression database to investigate the differential expression of KCND2 in breast cancer and normal breast tissue. In addition, we leveraged GO and KEGG analysis techniques to gain a better understanding of the potential functional enrichment of 500 genes related to KCND2. Our findings were validated using collected tissue samples and clinical data from hospitals showed that KCND2 is a crucial independent factor in the prognosis of breast cancer patients. The higher the expression of KCND2, the shorter the survival time of breast cancer patients. Colony formation assay confirmed that KCND2 promotes the proliferation of breast cancer cells, whereas transwell assay and wound healing assay verified that KCND2 promoted breast cancer invasion and migration. In addition, 5-Ethynyl-2′-deoxyuridine (EdU) and flow cytometry revealed that KCND2 affected the cycle changes of breast cancer cells and contributed to the G1/S phase transition of breast cancer cells. Overall, our study demonstrates that KCND2 holds a promising potential as a significant target for breast cancer diagnosis and therapy.
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Data availability
Some data used in this study were obtained from the publicly accessible websites (https://portal.gdc.com; https://www.genome.gov/Funded-Programs-Projects/Genotype-Tissue-Expression-Project ). Other data are available from Shengjie Yang on reasonable request.
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This work was funded by Beijing Municipal Science and Technology Project (Z211100002521011) and National Science and Technology Major Project (2017ZX09304026).
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SY and XW conceived and designed the experiments. SY, PZ, LQ, and YW completed cell experiments and collected relevant data. SY and YL analyzed the data and wrote the paper. All authors discussed and improved the article.
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This study was conducted in accordance with the Declaration of Helsinki and approved by the he Ethics Committee of Beijing Shijitan Hospital (sjtky11-lx-2022 (052)) and Ethics Committee of Shandong Cancer Hospital (SDTHEC20110324). The patients/participants provided their written informed consent to participate in this study. Written informed consent was obtained from the individual(s) for the publication of any potentially identifiable images or data included in this article.
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Supplementary 1
Five-hundred genes list (Supplementary 1) which were most relevant to KCND2 (XLSX 23 kb)
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Yang, S., Zhou, P., Qi, L. et al. Promoting proliferation and tumorigenesis of breast cancer: KCND2’s significance as a prognostic factor. Funct Integr Genomics 23, 257 (2023). https://doi.org/10.1007/s10142-023-01183-0
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DOI: https://doi.org/10.1007/s10142-023-01183-0