Abstract
Long noncoding RNAs play important roles in the occurrence and development of many malignant cancers. This study focuses on the effects of LINC01087 on gastric cancer and its underlying mechanism. In the present study, LINC01087 and CAAP1 were found to be upregulated, and miR-135a-5p was diminished in gastric cancer specimens and cells. Inhibition of LINC01087 resulted in cell proliferation inhibition and induced cell apoptosis through the intrinsic apoptosis signaling pathway, as evidenced by the activation of caspase-3 and caspase-9. An investigation of the signaling pathway revealed that the effects on proliferation and apoptosis following LINC01087 knockdown were mediated by suppression of CAAP1. Furthermore, application of a miR-135a-5p inhibitor or overexpression of CAAP1 could attenuate the apoptotic effect achieved by LINC01087 inhibition, confirming the involvement of miR-135a-5p/CAAP1 signaling in the occurrence of gastric cancer. In conclusion, the LINC01087/miR-135a-5p/CAAP1 axis modulates gastric cancer tumorigenesis and pathogenesis and presents new insight into gastric cancer targeted therapy.
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The data used to support the findings of this study are available from the corresponding author upon request.
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DZ designed the study. KZ, CD, and DZ performed the experiments and drafted the manuscript. QL and CD analyzed the data. DZ and KZ revised the manuscript.
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All procedures involving human participants were performed in accordance with the ethical standards of the Institutional and National Research Committee, and with the Declaration of Helsinki. Ethical approval was obtained from the Ethical Committee of Soochow Medical University. Verbal informed consent was obtained from all patients included in the present study.
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Zhang, KX., Ding, C., Liu, QH. et al. Knockdown of LINC01087 inhibits gastric cancer malignant behavior by regulating the miR-135a-5p/CAAP1 axis. Funct Integr Genomics 23, 248 (2023). https://doi.org/10.1007/s10142-023-01157-2
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DOI: https://doi.org/10.1007/s10142-023-01157-2