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Proteomic analysis of coarse and fine skin tissues of Liaoning cashmere goat

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Abstract

Proteomics is the study of all proteins expressed by a cell or even an organism. However, knowledge of proteins that regulate the fineness of cashmere is limited. Liaoning cashmere goat (LCG) is a valuable genetic resource of China. The skin samples of Liaoning cashmere goats during the growing period were collected, performed tandem mass tag (TMT) method, and identified 117 differentially expressed proteins in CT_LCG (course type) and FT_LCG (fine type). To verify proteins differentially expressed in LCG, we performed PRM validation on three candidate proteins (ALB, SDC1, and ITGB4) in CT-LCG and FT-LCG. Furthermore, primary metabolic process and lysosome are most enriched in the GO and KEGG pathways, respectively. In addition, we also derived a protein–protein interaction (PPI) regulatory network from the perspective of bioinformatics. This study sought to elucidate the molecular mechanism of differential proteins regulating cashmere fineness of Liaoning cashmere goats by using TMT quantitative proteomics analysis. Differentially expressed proteins ALB and SDC1 may regulate cashmere fineness; ITGB4 can become a promising protein for further study. They can be used as key proteins to lay a foundation for studying cashmere fineness of Liaoning cashmere goats.

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The data that support the findings of this study are available from the corresponding author upon reasonable request.

References

  • Almeida AM, Bassols A, Bendixen E, Bhide M, Ceciliani F, Cristobal S, Eckersall PD, Hollung K, Lisacek F, Mazzucchelli G, McLaughlin M, Miller I, Nally JE, Plowman J, Renaut J, Rodrigues P, Roncada P, Staric J, Turk R (2015) Animal board invited review: advances in proteomics for animal and food sciences. Animal 9:1–17

    Article  CAS  Google Scholar 

  • Bai WL (2006) Multivariate statistic analysis of morphological and ecological characters of cashmere goat populations in China. J Anhui Agric Sci 34:489

    Google Scholar 

  • Bai WL, Zhao SJ, Wang ZY et al (2018) LncRNAs in SHF of cashmere goat: identification, expression, and their regulatory network in wnt signaling pathway. Anim Biotechnol 29(3):199211

    Article  Google Scholar 

  • Bai D, Hao et al (2019) The overexpression of Tβ4 in the hair follicle tissue of alpas cashmere goats increases cashmere yield and promotes hair follicle development. [J]. Animals 10(1):75. https://doi.org/10.3390/ani10010075

  • Berger J, Buuveibaatar B, Mishra C (2013) Globalization of the cashmere market and the decline of large mammals in central Asia. Conserv Biol 27:679–689

    Article  Google Scholar 

  • Chen X, Zhao H, Chen C, Li J, He J, Fu X, Zhao H (2020) The HPA/SDC1 axis promotes invasion and metastasis of pancreatic cancer cells by activating EMT via FGF2 upregulation. Oncol Lett 19(1):211–220. https://doi.org/10.3892/ol.2019.11121

    Article  CAS  PubMed  Google Scholar 

  • Clerens S, Cornellison CD, Deb-Choudhury S, Thomas A, Plowman JE, Dyer JM (2010) Developing the wool proteome. J Proteome 73:1722–1731

    Article  CAS  Google Scholar 

  • Cui X, Xuan J, Qin Y et al (2017) Clinicopathological and prognostic significance of SDC1 overexpression in breast cancer [J]. Oncotarget 8(67):111444–111455

    Article  Google Scholar 

  • Fabre S, Chantepie L, Plisson-Petit F et al (2021) A novel homozygous nonsense mutation in ITGB4 gene causes epidermolysis bullosa in Mouton Vendéen sheep. Anim Genet 52(1):138–139. https://doi.org/10.1111/age.13026

    Article  CAS  PubMed  Google Scholar 

  • Franceschini A, Szklarczyk D, Frankild S, Kuhn M, Simonovic M, Roth A, Lin J, Minguez P, Bork P, von Mering C, Jensen LJ (2013) STRING v9.1: protein-protein interaction networks, with increased coverage and integration. Nucleic Acids Res 41(Database issue):D808–15. https://doi.org/10.1093/nar/gks109

  • Fu X, Zhao B, Tian K et al (2020) Integrated analysis of lncRNA and mRNA reveals novel insights into cashmere fineness in Tibetan cashmere goats [J]. PeerJ 8:e10217. https://doi.org/10.7717/peerj.10217

    Article  PubMed  PubMed Central  Google Scholar 

  • Geng R, Wang L, Wang X, Chen Y (2014) Cyclic expression of Lhx2 is involved in secondary hair follicle development in cashmere goat. Gene Expr Patterns 16(1):3135

    Article  Google Scholar 

  • He N, Su R, Wang Z, Zhang Y, Li J (2020) Exploring differentially expressed genes between anagen and telogen secondary hair follicle stem cells from the cashmere goat (Capra hircus) by RNA-Seq. PLoS ONE 15(4):e0231376. https://doi.org/10.1371/journal.pone.0231376

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Huang DW, Sherman BT, Lempicki RA (2009) Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists[J]. Nucleic Acids Res 37(1):1–13

    Article  Google Scholar 

  • Ji XY, Wang JX, Liu B, Zheng ZQ, Fu SY, Tarekegn GM, Bai X, Bai YS, Li H, Zhang WG (2016) Comparative transcriptome analysis reveals that a ubiquitin-mediated proteolysis pathway is important for primary and secondary hair follicle development in cashmere goats. PLoS ONE 11(10):e0156124. https://doi.org/10.1371/journal.pone.0156124

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jin M, Fu ZY, Luan YY (2006a) Cloning and evolution analysis of ZFX, ZFY partial gene in new-breeding cashmere goat and identification of its sex. Acta Vet Zootech Sin 37(6):530–536

    CAS  Google Scholar 

  • Jin M, Cui YH, Fu ZY et al (2006b) The correlation analysis of blood protein polymorphism with economics traits in Liaoning new-breeding cashmere goat. Hereditas (Beijing) 28(5):529–532

    CAS  Google Scholar 

  • Jin M, Cao M, Chu M, Piao J, Guo X, Zhao F, Piao J (2019) Gene expression and localization of LAMTOR3 in the skin cells of Liaoning cashmere goats. Anim Biotechnol 30(1):36–42. https://doi.org/10.1080/10495398.2018.1428198

    Article  CAS  PubMed  Google Scholar 

  • Jones P, Binns D, Chang HY et al (2014) InterProScan 5: genome-scale protein function classification[J]. Bioinformatics 30(9):1236–1240

    Article  CAS  Google Scholar 

  • Koehn H, Clerens S, Deb-Choudhury S, Morton JD, Dyer JM, Plowman JE (2010) The proteome of the wool cuticle. J Proteome Res 9:2920–2928

    Article  CAS  Google Scholar 

  • Li CQ, Yin J, Zhang YJ et al (2005) Comparative study on skin and hair follicles cycling between Inner Mongolia and Liaoning cashmere goats. Acta Vet Zootech Sin 36(7):674–679

    Google Scholar 

  • Li YS, Meng RR, Chen X et al (2018) Generation of H11-albumin-rtTA Transgenic Mice: a tool for inducible gene expression in the liver [J]. G3 (Bethesda) 9(2):g3.200963.2018

    Google Scholar 

  • Li J, Jiang Y, Chen C et al (2020) Integrin β4 is an effective and efficient marker in synchronously highlighting lymphatic and blood vascular invasion, and perineural aggression in malignancy. Am J Surg Pathol 44(5):681–690. https://doi.org/10.1097/PAS.0000000000001451

    Article  PubMed  Google Scholar 

  • Liao S, Liu C, Zhu G et al (2019) Relationship between SDC1 and cadherin signalling activation in cancer [J]. Pathol Res Pract 216(1):152756

    Article  Google Scholar 

  • Liu Z, Jin H, Yang S, Cao H, Zhang Z, Wen B, Zhou S (2020) SDC1 knockdown induces epithelial-mesenchymal transition and invasion of gallbladder cancer cells via the ERK/Snail pathway. J Int Med Res 48(8):300060520947883. https://doi.org/10.1177/0300060520947883

    Article  CAS  PubMed  Google Scholar 

  • MacLean B et al (2010) Skyline: an open source document editor for creating and analyzing targeted proteomics experiments. Bioinformatics 26(7):966–968

    Article  CAS  Google Scholar 

  • Malhotra A, Mittal BR (2014) SiRNA gene therapy using albumin as a carrier[J]. Pharmacogenet Genomics 24(12):582–587

    Article  CAS  Google Scholar 

  • Masukawa Y, Narita H, Imokawa G (2005) Characterization of the lipid composition at the proximal root regions of human hair. Int J Cosmet Sci 27:191–191

    Article  Google Scholar 

  • McLaren RJ, Rogers GR, Davies KP, Maddox JF, Montgomery GW (1997) Linkage mapping of wool keratin and keratin-associated protein genes in sheep. Mamm Genome 8:938–940

    Article  CAS  Google Scholar 

  • Miao C, Yang Y, Li S et al (2018) Discrimination and quantification of homologous keratins from goat and sheep with dual protease digestion and PRM assays [J]. J Proteomics 186:38–46

    Article  CAS  Google Scholar 

  • Peterson AC, Russell JD, Bailey DJ et al (2012) Parallel reaction monitoring for high resolution and high mass accuracy quantitative, targeted proteomics [J]. Mol Cell Proteomics 11(11):1475–1488

    Article  Google Scholar 

  • Piao J, Xu CL, Piao JA et al (2020) Expression analysis of proteasome maturation protein (POMP) gene in Liaoning cashmere goat [J]. Anim Biotechnol 31(4):324–334. https://doi.org/10.1080/10495398.2019.1596946

  • Plowman JE, Harland DP, Ganeshan S, Woods JL, van Shaijik B, Deb-Choudhury S, Thomas A, Clerens S, Scobie DR (2015) The proteomics of wool fibre morphogenesis. J Struct Biol 191:341–351

    Article  CAS  Google Scholar 

  • Soares R, Franco C, Pires E, Ventosa M, Palhinhas R, Koci K, Martinho de Almeida A, Varela Coelho A (2012) Mass spectrometry and animal science: protein identification strategies and particularities of farm animal species. J Proteomics 75:4190–4206

    Article  CAS  Google Scholar 

  • Su R, Fan Y, Qiao X, Li X, Zhang L, Li C, Li J (2018) Transcriptomic analysis reveals critical genes for the hair follicle of Inner Mongolia cashmere goat from catagen to telogen. PLoS ONE 13(10):e0204404. https://doi.org/10.1371/journal.pone.0204404

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Suárez-Vega A, Gutiérrez-Gil B, Benavides J et al (2015) Combining GWAS and RNA-Seq approaches for detection of the causal mutation for hereditary junctional epidermolysis bullosa in sheep [J]. PLoS ONE 10(5):e0126416

    Article  Google Scholar 

  • Wang X, Wang Y, Zheng X, Hao X, Liang Y, Wu M, Wang X, Wang Z (2014) Direct interaction between Ras homolog enriched in brain and FK506 binding protein 38 in cashmere goat fetal fibroblast cells. Asian-Australas J Anim Sci 27(12):1671–1677. https://doi.org/10.5713/ajas.2014.14145

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ware LB, Johnson A, Zager RA (2011) Renal cortical albumin gene induction and urinary albumin excretion in response to acute kidney injury. [J]. AJP Renal Physiol 300(3):F628–F638

    Article  CAS  Google Scholar 

  • Wu G, He Y (2009) Identification of varieties of cashmere by Vis/Nir spectroscopy technology based on Pca-Svm, vol 29. IEEE, Piscataway, pp 1541–1544

    Google Scholar 

  • Yu L, Xu H, Zhang S et al (2020) SDC1 promotes cisplatin resistance in hepatic carcinoma cells via PI3K-AKT pathway [J]. Hum Cell 33(3):721–729

    Article  CAS  Google Scholar 

  • Yuan C, Wang X, Geng R, He X, Qu L, Chen Y (2013) Discovery of cashmere goat (Capra hircus) microRNAs in skin and hair follicles by Solexa sequencing. BMC Genomics 14:511. https://doi.org/10.1186/1471-2164-14-511

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zheng YY, Sheng SD, Hui TY, Yue C, Sun JM, Guo D, Guo SL, Li BJ, Xue HL, Wang ZY, Bai WL (2019) An integrated analysis of cashmere fineness lncRNAs in cashmere goats. Genes (Basel) 10(4):266. https://doi.org/10.3390/genes10040266

    Article  CAS  Google Scholar 

  • Zhu B, Xu T, Yuan J, Guo X, Liu D (2013) Transcriptome sequencing reveals differences between primary and secondary hair follicle-derived dermal papilla cells of the cashmere goat (Capra hircus). PLoS ONE 8(9):e76282. https://doi.org/10.1371/journal.pone.0076282

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

1. Thanks to the teachers and students of Shenyang Agricultural University in the technical help, including instruments and equipment and their related experimental materials, collaborative experimental work, to provide useful inspiration, suggestions, guidance, review, undertake some auxiliary work, etc.

2. Thanks to the Foundation for helping the Grants from Postdoctoral Science Foundation of China: Genetic trajectory and expression localization of key genes of cashmere fineness by multi-omics, Project No. 2021M693859, 2021 Liaoning Province “ the open competition mechanism to select the best candidates” Science and Technology Research Project: Selection and breeding of special advantageous livestock and poultry breeds in Liaoning and key technology of whole industry chain production, Project No. 2021JH1/10400033 and National modern agricultural industrial technology system, project number: cars-39-27.

Funding

Our scientific research was financially aided by four projects: (1) Postdoctoral Science Foundation of China: Genetic trajectory and expression localization of key genes of cashmere fineness by multi-omics, Project No. 2021M693859. (2) 2021 Liaoning Province “ the open competition mechanism to select the best candidates” Science and Technology Research Project: Selection and breeding of special advantageous livestock and poultry breeds in Liaoning and key technology of whole industry chain production, Project No. 2021JH1/10400033. (3) National modern agricultural industrial technology system, project number: cars-39–27.

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Authors and Affiliations

  1. College of Animal Science &Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China

    Zhixian Bai, Yanan Xu, Ming Gu, Weidong Cai, Yu Zhang, Yuting Qin, Rui Chen, Yinggang Sun, Yanzhi Wu & Zeying Wang

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  1. Zhixian Bai
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  2. Yanan Xu
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Contributions

Data curation, ZXB; formal analysis, YNX and GM; funding acquisition, ZYW; investigation, ZXB; methodology, ZYW; project administration, ZYW; resources, WDC and XJZ; software, ZXB, YTQ, YZ, RC, YGS and YZW; supervision, ZYW; validation, ZXB; visualization, ZYW; Writing — original draft, ZXB; writing — review & editing, ZXB and ZY.

Corresponding author

Correspondence to Zeying Wang.

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All Liaoning cashmere goat experiments used in this study were conducted in accordance with the guidelines of the Laboratory Animal Management Committee of Shenyang Agricultural University.

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Statement

In this work, we identified 117 differentially expressed proteins in CT_LCG (course type) and FT_LCG (fine type). To verify protein genes differentially expressed in LCG, we performed PRM validation on three candidate proteins (ALB, SDC1 and ITGB4) in CT-LCG and FT-LCG. His study sought to elucidate the molecular mechanism of differential proteins regulating cashmere fineness of Liaoning cashmere goats by using TMT quantitative proteomics analysis. Differentially expressed proteins ALB and SDC1 may regulate cashmere fineness; ITGB4 can be further studied as a promising protein. They can be used as key genes to lay a foundation for the study of cashmere fineness of Liaoning cashmere goats.

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Bai, Z., Xu, Y., Gu, M. et al. Proteomic analysis of coarse and fine skin tissues of Liaoning cashmere goat. Funct Integr Genomics 22, 503–513 (2022). https://doi.org/10.1007/s10142-022-00856-6

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