Abstract
Phytohormones play an important role in the pollination and fertilization of crops, but the regulatory mechanisms of oil palm pollination and fertilization are unclear. The purpose of this study is to explore the hormonal changes of oil palm pistils during flowering. We used RNA sequencing to evaluate differentially expressed genes (DEGs) in oil palm pistils at the pollination and non-pollination stages. In this study, we found that the hormone contents of oil palm pistil changed drastically after pollination. The transcriptome of the oil palm pistil without pollination and at 2 h, 4 h, 12 h, 24 h, and 48 h after pollination was comprehensively analyzed, and a large number of differential genes and metabolic pathways were explored. Based on the transcriptome data, it could be recognized that the changes of indoleacetic acid (IAA), zeatin riboside (ZR), and abscisic acid (ABA) during pollination were consistent with the changes in the corresponding gene transcripts. Differentially expressed genes during pollination and fertilization of oil palm were mainly related to energy metabolism and hormone signal transduction. It provides new insights to elucidate the interaction and regulation mechanisms of plant hormones before and after oil palm pollination, providing a theoretical basis and reference for the research on sexual reproduction of oil palm.
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Acknowledgements
We would like to express our gratitude to MetWare Biotechnology Co., Ltd. (Wuhan, China) for transcriptome sequencing assistance. This research was supported by Central Public-interest Scientific Institution Basal Research Fund for the Chinese Academy of Tropical Agricultural Sciences (No.1630052021028 and No. 17CXTD-13).
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This research was supported by Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (No. 1630052021028 and No. 17CXTD-13).
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MDY and RNZ designed and conducted the experiment, completed data analysis, and prepared the manuscript. YR and JJJM revised and corrected the manuscript. LFJ and LXZ participated in the experimental design and result analysis. HXC conceived the whole project, guided experimental design, data analysis, and manuscript writing. All authors have read and approved the final manuscript.
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Transcriptomic study revealed the role of phytohormone biosynthesis and signaling genes during the pollination and fertilization of oil palm plants
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Yang, M., Yarra, R., Zhang, R. et al. Transcriptome analysis of oil palm pistil during pollination and fertilization to unravel the role of phytohormone biosynthesis and signaling genes. Funct Integr Genomics 22, 261–278 (2022). https://doi.org/10.1007/s10142-022-00834-y
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DOI: https://doi.org/10.1007/s10142-022-00834-y