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Genome-wide comparative analysis of DNAJ genes and their co-expression patterns with HSP70s in aestivation of the sea cucumber Apostichopus japonicus

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Abstract

DNAJ proteins function as co-chaperones of HSP70 and play key roles in cell physiology to promote protein folding and degradation, especially under environmental stress. Based on our previous study on HSP70, a systematic study of DNAJ was performed in sea cucumber Apostichopus japonicus using the transcriptomic and genomic data, identifying 43 AjDNAJ genes, including six AjDNAJA genes, eight AjDNAJB genes, and 29 AjDNAJC genes. Slight expansion and conserved genomic structure were observed using the phylogenetic and syntenic analysis. Differential period-specific and tissue-specific expression patterns of AjDNAJs were observed between adult and juvenile individuals during aestivation. Strong tissue-specific expression correlations between AjDNAJ and AjHSP70 genes were found, indicating that the involvements of AjHSP70IVAs in the aestivation of sea cucumbers were regulated by AjDNAJs. Several key genes with significant expression correlations, such as AjDNAJB4L and AjHSP70IVAs, were suggested to function together under heat stress. Together, these findings provide early insight into the involvement of AjDNAJs in the aestivation and their roles as co-chaperones of AjHSP70s.

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Funding

This research was supported by the National Key R&D Program of China (2019YFD0901002), the Natural Science Foundation of Liaoning Province (2019-ZD-0725), the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (GML2019ZD0402), Dalian Qing Nian Ke Ji Zhi Xing Program (2018RQ20), the National Natural Science Foundation of China (Grant No. 31602155), the Liaoning BaiQianWan Talents Program (2018921039), and the Science and Technology Innovation Fund of Dalian (2021JJ13SN73).

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Authors and Affiliations

  1. Dalian Ocean University, Dalian, 116023, Liaoning, China

    Lei Gao & Zhen Ma

  2. Liaoning Ocean and Fisheries Science Research Institute, Dalian, 116023, Liaoning, China

    Lei Gao & Yunfeng Li

  3. The University of Texas Health Science Center at Houston, Houston, TX, 77030, USA

    Zihao Yuan

  4. The Fish Molecular Genetics and Biotechnology Laboratory, Aquatic Genomics Unit, School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA

    Zihao Yuan

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  1. Lei Gao
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Contributions

L.G. and Z.M. designed the project. L.G. and Y.L. collected the samples. L.G. and Z.Y. participated in data analyses. L.G. and Z.M. wrote the manuscript. All authors have read and approved the final manuscript.

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Correspondence to Lei Gao or Zhen Ma.

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Gao, L., Yuan, Z., Li, Y. et al. Genome-wide comparative analysis of DNAJ genes and their co-expression patterns with HSP70s in aestivation of the sea cucumber Apostichopus japonicus. Funct Integr Genomics 22, 317–330 (2022). https://doi.org/10.1007/s10142-022-00830-2

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