Abstract
Recently, the same fish diseases, which have been found in pond farming, have been found in the newly tested largemouth bass (Micropterus salmoides) system. Bacterial septicemia caused by Aeromonas hydrophila occurs frequently in largemouth bass culture leading to significant economic losses. To investigate the role miRNA in the largemouth bass disease resistance, twelve (2 tissues (spleen and head kidney) × 2 experimental groups (infected and control) × three biological replicates) small RNA libraries were constructed and sequenced with miRNA-seq. A total of 26 differentially expressed miRNAs, 8 upregulated and 18 downregulated, were identified in the spleen, and 19 differentially expressed miRNAs, 9 upregulated and 10 downregulated, were identified in head kidney (fold change ≥ 2 or ≤ 0.5 and P ≤ 0.05). The differentially expressed miRNAs with the largest fold change were selected for target gene prediction using GO and KEGG analysis. Six miRNAs in the spleen and 5 miRNAs in the head kidney were selected. Analysis showed that, of all the immune and metabolic pathways, the FoxO signaling pathway was enriched in both the spleen and head kidney groups. Common target genes of the pathway included AMP-activated catalytic subunit alpha 1 (prkaa1), phosphatidylinositol 3-kinase (pik3r3b), serine/threonine-protein kinase (plk2), and forkhead box protein G1 (foxg1a). MiRNAs (such as miR-126-5P, miR-126-3P) are involved in immune response and cell cycle functions as they regulate targeted genes in the FoxO pathway. These results will enhance our understanding of the molecular mechanisms underlying immune responses to bacterial septicemia and facilitate molecular-assisted selection of resistant strains of largemouth bass.
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We are uploading the sequencing data to NCBI, and we will soon get the search number. Availability of data and materials: All data generated and analyzed during this study are included in this published article.
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This work was financially supported by the Agriculture Research System of China (CARS-46).
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Jian Zhou, Han Zhao, and Song Yang designed the experiment and wrote the manuscript. Lu Zhang, Qiang Li, Zhipeng Huang, and Zhongmeng Zhao carried out the experiments data organization and statistical analyses. Hongyu Ke, Yu Xiao, and Xutao Su provided the experimental samples. Han Zhao Qiao Liu and Liulan Zhao participated in the study design and discussed the manuscript. All authors read and approved the final manuscript.
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Zhou, J., Zhao, H., Zhang, L. et al. MiRNA-seq analysis of spleen and head kidney tissue from aquacultured largemouth bass (Micropterus salmoides) in response to Aeromonas hydrophila infection. Funct Integr Genomics 21, 101–111 (2021). https://doi.org/10.1007/s10142-020-00763-8
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DOI: https://doi.org/10.1007/s10142-020-00763-8