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A transcriptomic study of selenium against liver injury induced by beta-cypermethrin in mice by RNA-seq

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Evidence from biochemical liver function index and histopathology analysis suggested that selenium could effectively repair the liver injury caused by beta-cypermethrin (β-CYP). However, the molecular mechanism of selenium against liver injury induced by β-CYP remains unclear. In the present study, dynamic changes in gene expression profiles before and after the treatment of Na2SeO3 in liver injury mice were analyzed by using RNA sequencing. As a result, several essential genes and pathways were identified to be significantly associated with this process. In particular, ten genes including Cyp2j11, Cyp2b10, Cyp3a13, Dhrs9, Socs2, Stat4, Gm13305, Cyp3a44, Retsat, and Cyp26b1 were significantly enriched in the functional categories related to retinol metabolism, linoleic acid metabolism, and Jak-STAT signaling pathway. Among them, the expression patterns of nine genes were validated by qRT-PCR, except for Cyp3a44. Furthermore, we have constructed the associated regulatory network based on the identified targets revealed by high throughput screening. Our study may provide insight into the molecular mechanism underlying the protective effect of selenium against liver injury induced by β-CYP in mammals.

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This study was supported by grants from the Tackle key Problem Project in Science and Technology in Anhui Province, China (1501041177), the Promoting Project for Team Construction of Anhui Province, China (Y05201374), the Natural Science Foundation Project of Anhui Province (1508085QC63 and 1908085MC87), the Scientific Research Foundation and Academic & Technology Leaders Introduction Project, and the 211 Project of Anhui University (10117700023). We also thank the supports by the Natural Science Foundation of China (Grant No. 81570376 and 81870307).

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KH and BH designed the research. QT, MG, SY, XC, HZ, and DL performed the experiments and/or data analysis. KH, QT, MG, and BH wrote the paper with input from all authors.

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Correspondence to Bei Huang.

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He, K., Tang, Q., Gong, M. et al. A transcriptomic study of selenium against liver injury induced by beta-cypermethrin in mice by RNA-seq. Funct Integr Genomics 20, 343–353 (2020).

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