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A transcriptomic study of selenium against liver injury induced by beta-cypermethrin in mice by RNA-seq

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Abstract

Evidence from biochemical liver function index and histopathology analysis suggested that selenium could effectively repair the liver injury caused by beta-cypermethrin (β-CYP). However, the molecular mechanism of selenium against liver injury induced by β-CYP remains unclear. In the present study, dynamic changes in gene expression profiles before and after the treatment of Na2SeO3 in liver injury mice were analyzed by using RNA sequencing. As a result, several essential genes and pathways were identified to be significantly associated with this process. In particular, ten genes including Cyp2j11, Cyp2b10, Cyp3a13, Dhrs9, Socs2, Stat4, Gm13305, Cyp3a44, Retsat, and Cyp26b1 were significantly enriched in the functional categories related to retinol metabolism, linoleic acid metabolism, and Jak-STAT signaling pathway. Among them, the expression patterns of nine genes were validated by qRT-PCR, except for Cyp3a44. Furthermore, we have constructed the associated regulatory network based on the identified targets revealed by high throughput screening. Our study may provide insight into the molecular mechanism underlying the protective effect of selenium against liver injury induced by β-CYP in mammals.

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References

  • Altucci L, Leibowitz MD, Ogilvie KM, de Lera AR, Gronemeyer H (2007) RAR and RXR modulation in cancer and metabolic disease. Nat Rev Drug Discov 6:793–810

    Article  CAS  Google Scholar 

  • Chandra V, Wu D, Li S, Potluri N, Kim Y, Rastinejad F (2017) The quaternary architecture of RARbeta-RXRalpha heterodimer facilitates domain-domain signal transmission. Nat Commun 8:868

    Article  Google Scholar 

  • Chen L, Xu P, Diao J, Di S, Li R, Zhou Z (2016) Distribution, metabolism and toxic effects of beta-cypermethrin in lizards (Eremias argus) following oral administration. J Hazard Mater 306:87–94

    Article  CAS  Google Scholar 

  • Dennis G Jr, Sherman BT, Hosack DA, Yang J, Gao W, Lane HC, Lempicki RA (2003) DAVID: Database for annotation, visualization, and integrated discovery. Genome Biol 4:P3

    Article  Google Scholar 

  • Diel F, Horr B, Borck H, Irman-Florjanc T (2003) Pyrethroid insecticides influence the signal transduction in T helper lymphocytes from atopic and nonatopic subjects. Inflamm Res 52:154–163

    Article  CAS  Google Scholar 

  • Dobie R, MacRae VE, Pass C, Milne EM, Ahmed SF, Farquharson C (2018) Suppressor of cytokine signaling 2 (Socs2) deletion protects bone health of mice with DSS-induced inflammatory bowel disease. 11

  • Gao B (2005) Cytokines, STATs and liver disease. Cell Mol Immunol 2:92–100

    CAS  PubMed  Google Scholar 

  • Hou Z, Qin P, Ren G (2010) Effect of anthocyanin-rich extract from black rice (Oryza sativa L. Japonica) on chronically alcohol-induced liver damage in rats. J Agric Food Chem 58:3191–3196

    Article  CAS  Google Scholar 

  • Huang da W, Sherman BT, Lempicki RA (2009) Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc 4:44–57

    Article  Google Scholar 

  • Huang P, Chandra V, Rastinejad F (2014) Retinoic acid actions through mammalian nuclear receptors. Chem Rev 114:233–254

    Article  CAS  Google Scholar 

  • Lestremau F, Willemin ME, Chatellier C, Desmots S, Brochot C (2014) Determination of cis-permethrin, trans-permethrin and associated metabolites in rat blood and organs by gas chromatography-ion trap mass spectrometry. Anal Bioanal Chem 406:3477–3487

    Article  CAS  Google Scholar 

  • Liu L, Hu JX, Wang H, Chen BJ, He Z, Xu LC (2010) Effects of beta-cypermethrin on male rat reproductive system. Environ Toxicol Pharmacol 30:251–256

    Article  CAS  Google Scholar 

  • Macrae VE, Horvat S, Pells SC, Dale H, Collinson RS, Pitsillides AA, Ahmed SF, Farquharson C (2009) Increased bone mass, altered trabecular architecture and modified growth plate organization in the growing skeleton of SOCS2 deficient mice. J Cell Physiol 218:276–284

    Article  CAS  Google Scholar 

  • Masuzaki R, Zhao S, Valerius MT, Tsugawa D, Oya Y, Ray KC, Karp SJ (2016) SOCS2 balances metabolic and restorative requirements during liver regeneration. J Biol Chem 291:3346–3358

    Article  CAS  Google Scholar 

  • Mikata K, Isobe N, Kaneko H (2012) Biotransformation and enzymatic reactions of synthetic pyrethroids in mammals. Top Curr Chem 314:113–135

    Article  CAS  Google Scholar 

  • Mu X, Shen G, Huang Y, Luo J, Zhu L, Qi S, Li Y, Wang C, Li X (2017) The enantioselective toxicity and oxidative stress of beta-cypermethrin on zebrafish. Environ Pollut (Barking, Essex : 1987) 229:312–320

    Article  CAS  Google Scholar 

  • Newairy AA, El-Sharaky AS, Badreldeen MM, Eweda SM, Sheweita SA (2007) The hepatoprotective effects of selenium against cadmium toxicity in rats. Toxicology 242:23–30

    Article  CAS  Google Scholar 

  • Ratelle M, Cote J, Bouchard M (2015) Toxicokinetics of permethrin biomarkers of exposure in orally exposed volunteers. Toxicol Lett 232:369–375

    Article  CAS  Google Scholar 

  • Rayman MP (2000) The importance of selenium to human health. Lancet 356:233–241

    Article  CAS  Google Scholar 

  • Riquelme P, Amodio G, Macedo C, Moreau A, Obermajer N, Brochhausen C, Ahrens N, Kekarainen T, Fandrich F, Cuturi C, Gregori S, Metes D, Schlitt HJ, Thomson AW, Geissler EK, Hutchinson JA (2017) DHRS9 is a stable marker of human regulatory macrophages. Transplantation 101:2731–2738

    Article  CAS  Google Scholar 

  • Schomburg L, Kohrle J (2008) On the importance of selenium and iodine metabolism for thyroid hormone biosynthesis and human health. Mol Nutr Food Res 52:1235–1246

    Article  CAS  Google Scholar 

  • Schupp M, Lefterova MI, Janke J, Leitner K, Cristancho AG, Mullican SE, Qatanani M, Szwergold N, Steger DJ, Curtin JC, Kim RJ, Suh MJ, Albert MR, Engeli S, Gudas LJ, Lazar MA (2009) Retinol saturase promotes adipogenesis and is downregulated in obesity. Proc Natl Acad Sci U S A 106:1105–1110

    Article  CAS  Google Scholar 

  • Scollon EJ, Starr JM, Godin SJ, DeVito MJ, Hughes MF (2009) In vitro metabolism of pyrethroid pesticides by rat and human hepatic microsomes and cytochrome p450 isoforms. Drug Metab Dispos 37:221–228

    Article  CAS  Google Scholar 

  • Shen XD, Ke B, Zhai Y, Gao F, Anselmo D, Lassman CR, Busuttil RW, Kupiec-Weglinski JW (2003) Stat4 and Stat6 signaling in hepatic ischemia/reperfusion injury in mice: HO-1 dependence of Stat4 disruption-mediated cytoprotection. Hepatology (Baltimore, Md) 37:296–303

    Article  CAS  Google Scholar 

  • Starr JM, Scollon EJ, Hughes MF, Ross DG, Graham SE, Crofton KM, Wolansky MJ, Devito MJ, Tornero-Velez R (2012) Environmentally relevant mixtures in cumulative assessments: an acute study of toxicokinetics and effects on motor activity in rats exposed to a mixture of pyrethroids. Toxicol Sci 130:309–318

    Article  CAS  Google Scholar 

  • Uzunhisarcikli M, Aslanturk A, Kalender S, Apaydin FG, Bas H (2016) Mercuric chloride induced hepatotoxic and hematologic changes in rats: the protective effects of sodium selenite and vitamin E. Toxicol Ind Health 32:1651–1662

    Article  CAS  Google Scholar 

  • Wang XZ, Liu SS, Sun Y, Wu JY, Zhou YL, Zhang JH (2009) Beta-cypermethrin impairs reproductive function in male mice by inducing oxidative stress. Theriogenology 72:599–611

    Article  CAS  Google Scholar 

  • Wang X, Gao X, He B, Jin Y, Fu Z (2017a) Cis-bifenthrin causes immunotoxicity in murine macrophages. Chemosphere 168:1375–1382

    Article  CAS  Google Scholar 

  • Wang X, He B, Kong B, Wei L, Wang R, Zhou C, Shao Y, Lin J, Jin Y, Fu Z (2017b) beta-Cypermethrin and its metabolite 3-phenoxybenzoic acid exhibit immunotoxicity in murine macrophages. Acta Biochim Biophys Sin 49:1083–1091

    Article  CAS  Google Scholar 

  • Zhang F, Zhang J, Li Y (2012) Corn oligopeptides protect against early alcoholic liver injury in rats. Food Chem Toxicol 50:2149–2154

    Article  CAS  Google Scholar 

  • Zhang M, Liu C, Hu MY, Zhang J, Xu P, Li F, Zhong ZY, Liu L, Liu XD (2015a) High-fat diet enhanced retinal dehydrogenase activity, but suppressed retinol dehydrogenase activity in liver of rats. J Pharmacol Sci 127:430–438

    Article  CAS  Google Scholar 

  • Zhang L, Wang K, Lei Y, Li Q, Nice EC, Huang C (2015b) Redox signaling: potential arbitrator of autophagy and apoptosis in therapeutic response. Free Radic Biol Med 89:452–465

    Article  CAS  Google Scholar 

  • Zhang Y, Zhou Y, Tang Q, Hu F, Feng L, Shen J, Huang B (2018) The protective effects of selenium-enriched spirulina on the reproductive system of male zebrafish (Danio rerio) exposed to beta-cypermethrin. Food Funct

  • Zhou YJ, Wang XD, Xiao S, Yu DE, Wang LQ, Wang JH, Zhu HQ (2018a) Exposure to beta-cypermethrin impairs the reproductive function of female mice. Regul Toxicol Pharmacol 95:385–394

    Article  CAS  Google Scholar 

  • Zhou YJ, Huang HR, Zhou J, Wang LQ (2018b) Beta-cypermethrin exposure affects female reproduction by enhancing oxidative stress in mice uterine tissue. Regul Toxicol Pharmacol 98:284–290

    Article  CAS  Google Scholar 

  • Zhou YJ, Wang JH, Wang LQ, Xiao S, Wang XD, Yan HL, Li CF, Zhu HQ (2018c) Effect of beta-cypermethrin exposure on embryo implantation in mice. Reprod Toxicol (Elmsford, NY) 76:1–11

    Article  CAS  Google Scholar 

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Funding

This study was supported by grants from the Tackle key Problem Project in Science and Technology in Anhui Province, China (1501041177), the Promoting Project for Team Construction of Anhui Province, China (Y05201374), the Natural Science Foundation Project of Anhui Province (1508085QC63 and 1908085MC87), the Scientific Research Foundation and Academic & Technology Leaders Introduction Project, and the 211 Project of Anhui University (10117700023). We also thank the supports by the Natural Science Foundation of China (Grant No. 81570376 and 81870307).

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KH and BH designed the research. QT, MG, SY, XC, HZ, and DL performed the experiments and/or data analysis. KH, QT, MG, and BH wrote the paper with input from all authors.

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Correspondence to Bei Huang.

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He, K., Tang, Q., Gong, M. et al. A transcriptomic study of selenium against liver injury induced by beta-cypermethrin in mice by RNA-seq. Funct Integr Genomics 20, 343–353 (2020). https://doi.org/10.1007/s10142-019-00719-7

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  • DOI: https://doi.org/10.1007/s10142-019-00719-7

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