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Functional & Integrative Genomics

, Volume 19, Issue 3, pp 467–486 | Cite as

Proteomic and transcriptomic analysis to unravel the influence of high temperature on banana fruit during postharvest storage

  • Taotao Li
  • Qixian Wu
  • Xuewu Duan
  • Ze YunEmail author
  • Yueming Jiang
Original Article
  • 291 Downloads

Abstract

Banana, an important food, incurs significant economic losses due to high storage temperature. Integrative analysis of proteome and transcriptome profiles of the banana peel stored at 20 °C (control) and 30 °C (HT) was used to investigate the molecular mechanism in response to high temperature stress. Critical proteins and genes relating to the response of banana fruit to HT stress were evaluated using partial least squares-discriminant analysis (PLS-DA) and orthogonal signal correction partial least squares-discriminant analysis (OPLS-DA). HT stress influenced proteins/genes related to chlorophyll metabolism, fruit firmness, signal transduction, energy metabolism, and stress response and defense. Together with scanning electron microscopy (SEM) and real time quantitative PCR (RT-qPCR) results, it can be concluded that HT stress resulted in stay-green ripening of banana fruit. Additionally, HT stress accelerated firmness loss and senescence of banana peel, might mainly through regulating hormone signaling pathway, stress protective ability, and energy metabolism in the banana peel. Our study provided a clearer understanding of regulatory mechanisms of HT treatment on banana fruit and potential genetic resources for the improvement of high temperature-tolerant characteristics in banana fruit.

Keywords

Fruit Temperature Transcriptome Proteome Stress 

Notes

Acknowledgments

We extend sincere thanks and gratitude to Afiya John, a native English speaker, for her kind assistance in revision and editing of this manuscript.

Funding information

This work was supported by Pearl River S&T Nova Program of Guangzhou (No.201610010041), National Natural Science Foundation of China (Grant Nos. 31671911, 31701657), Young Elite Scientists Sponsorship Program by CAST (2017QNRC001), and Key Laboratory of Post-Harvest Handling of fruits, Ministry of Agriculture.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Fig. S1 (DOCX 625 kb)
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Table S1 (DOCX 14 kb)
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Table S2 (XLSX 100 kb)
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Table S3 (XLSX 170 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Taotao Li
    • 1
  • Qixian Wu
    • 1
    • 2
  • Xuewu Duan
    • 1
  • Ze Yun
    • 1
    Email author
  • Yueming Jiang
    • 1
  1. 1.Key Laboratory of Plant Resource Conservation and Sustainable Utilization, South China Botanical GardenChinese Academy of SciencesGuangzhouChina
  2. 2.College of Life ScienceUniversity of Chinese Academy of SciencesBeijingChina

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