Functional & Integrative Genomics

, Volume 18, Issue 6, pp 701–707 | Cite as

Differential expression of miR-let7a in hair follicle cycle of Liaoning cashmere goats and identification of its targets

  • Tao Ma
  • Jianping Li
  • Qian Jiang
  • Sufang Wu
  • Huaizhi Jiang
  • Qiaoling Zhang
Original Article


In order to improve the production and quality of Chinese cashmere, the research of hair follicle development has aroused more and more attention; the regulation mechanism of miRNA in hair follicle development has become a hot spot. A survey of transcriptome profiling screened 10 hair follicle-related miRNAs that were differentially expressed, including miR-let7a. In this study, the expression of miR-let7a was lower in anagen of hair follicle of cashmere goats than that in catagen of hair follicle of cashmere goats (p < 0.01). Results were in accordance with transcriptome data. The expression patterns of miR-let7a target genes (IGF-1R, C-myc, and FGF5) were verified by qRT-PCR, which were consistent with the results of Western blot and showed a downward trend. The dual-luciferase reporter gene system was used to verify the correlation between the expression of miR-let7a and its target genes, and it showed that miR-let7a negatively correlates with C-myc and FGF5. Present study offers new information on miRNAs and their related target genes in the regulation of hair follicle development mechanism.


Liaoning cashmere goats miR-let7a Hair follicle development Differential expression 



This research was supported by the Cashmere Goat Breeding Center of Liaoning province.

Funding information

This work was supported by the Jilin Province Natural Science Foundation (20170101156JC), the Special Funds for Scientific Research on Public Causes (201303119), the Special Foundation for Postdoctor of China Ministry of Education (No. 20100471261), and the grants from the National Natural Science Foundation of China (NSFC) (Nos. 30800807 and 31072097).

Compliance with ethical standards

This study was conducted in strict compliance with the recommendations of under the guidance of Jilin University Animal Care and Use Committee.

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Abramov R, Fu G, Zhang Y, Peng C (2013) Expression and regulation of miR-17a and miR-430b in zebrafish ovarian follicles. Gen Comp Endocrinol 188:309–315CrossRefGoogle Scholar
  2. Ahmed MI, Mardaryev AN, Lewis CJ, Sharov AA, Botchkareva NV (2011) Micro RNA-21 is an important downstream component of BMP signaling in epidermal keratinocytes. J Cell Sci 124(Pt 20):3399–3404CrossRefGoogle Scholar
  3. Andl T, Murchison EP, Liu F, Zhang Y, Yunta-Gonzalez M, Tobias JW, Andl CD, Seykora JT, Hannon GJ, Millar SE (2006) The miRNA-processing enzyme dicer is essential for the morphogenesis and maintenance of hair follicles. Curr Biol 16(10):1041–1049CrossRefGoogle Scholar
  4. Collu GM, Hidalgo-Sastre A, Brennan K (2014) Wnt-notch signalling crosstalk in development and disease. Cell Mol Life Sci 71(18):3553–3567CrossRefGoogle Scholar
  5. Elhamamsy AR, El Sharkawy MS, Zanaty AF et al (2017) Circulating miR-92a, miR-143 and miR-342 in plasma are novel potential biomarkers for acute myeloid leukemia. Int J Mol Cell Med 6(2):77–86PubMedPubMedCentralGoogle Scholar
  6. Estrach S, Ambler CA, Lo Celso C et al (2006) Jagged 1 is a beta-catenin target gene required for ectopic hair follicle formation in adult epidermis. Development 133(22):4427–4438CrossRefGoogle Scholar
  7. Frucht CS, Santos-Sacchi J, Navaratnam DS (2011) MicroRNA181a play a key role in hair cell regeneration in the avian auditory epithelium. Neurosci Lett 493(1–2):44–48CrossRefGoogle Scholar
  8. Isik M, Berezikov E (2013) Expression pattern analysis of microRNAs in Caenorhabditis elegans. Methods Mol Biol 936:129–141CrossRefGoogle Scholar
  9. Jin M (2016) The study on biological function of keratin 26, a novel member of Liaoning cashmere goat keratin gene family. PLoS One 11(12):e0168015CrossRefGoogle Scholar
  10. Ku AT, Mia Q, Nguyen H (2016) Monitoring Wnt/β-catenin signaling in skin. Methods Mol Biol 1481:127–140CrossRefGoogle Scholar
  11. Kurek D, Garinis GA, van Doorninck JH, van der Wees J, Grosveld FG (2007) Transcriptome and phenotypic analysis reveals Gata3-dependent signalling pathways in murine hair follicles. Development 134(2):261–272CrossRefGoogle Scholar
  12. Li JP, Qu HE, Jiang HZ et al (2015) Transcriptome-wide comparative analysis of microRNA profiles in the Telogen skins of Liaoning cashmere goats (Capra hircus) and fine-wool sheep (Ovis aries) by Solexa deep sequencing. DNA Cell Biol 35(11):696–705CrossRefGoogle Scholar
  13. Lv X, Sun W, Yin J, Ni R, Su R, Wang Q, Gao W, Bao J, Yu J, Wang L, Chen L (2016) An integrated analysis of microRNA and mRNA expression profiles to identify RNA expression signatures in lambskin hair follicles in Hu sheep. PLoS One 11(7):e0157463CrossRefGoogle Scholar
  14. Mardaryev AN, Ahmed MI, Vlahov NV, Fessing MY, Gill JH, Sharov AA, Botchkareva NV (2010) Micro-RNA-31 controls hair cycle-associated changes in gene expression programs of the skin and hair follicle. FASEB J 24(10):3869–3881CrossRefGoogle Scholar
  15. Qiu W, Lei M, Zhou L, Bai X, Lai X, Yu Y, Yang T, Lian X (2017) Hair follicle stem cell proliferation, Akt and Wnt signaling activation in TPA-induced hair regeneration. Histochem Cell Biol 147(6):749–758CrossRefGoogle Scholar
  16. Qu HE (2014) The analysis of differential expression of miRNA-125b on the skin of cashmere goats and fine-wool sheep, and screen or identify its targets. Jilin University, ChangchunGoogle Scholar
  17. Qu L, Li JP, Zhao ZH et al (2017) Differential expression of miR-202 and validation of predicted target genes in the skin tissue of C57BL/6 black mice and BALB/c white mice. DNA Cell Biol 36(6):443–450CrossRefGoogle Scholar
  18. Sehultz J, Lorenz P, Gross G et al (2008) MicroRNA let-7b targets important cell cycle molecules in malignant melanoma cells and interferes with anchorage-independent growth. Cell Res 18(5):549–557CrossRefGoogle Scholar
  19. Soma T, Fujiwara S, Shirakata Y et al (2012) Hair-inducing ability of human dermal papilla cells cultured under Wnt/β-catenin signalling activation. Exp Dermatol 21(4):307–309CrossRefGoogle Scholar
  20. Song J, Lee JE (2015) ASK1 modulates the expression of microRNA Let7A in microglia under high glucose in vitro condition. Front Cell Neurosci 9:198PubMedPubMedCentralGoogle Scholar
  21. Telerman SB, Rognoni E, Sequeira I, Pisco AO, Lichtenberger BM, Culley OJ, Viswanathan P, Driskell RR, Watt FM (2017) Dermal Blimp1 acts downstream of epidermal TGFβ and Wnt/β-catenin to regulate hair follicle formation and growth. J Invest Dermatol 137(11):2270–2281CrossRefGoogle Scholar
  22. Tian X, Jiang JB, Fan RW et al (2012) Identification and characterization of mi RNAs in white and brown alpaca skin. BMC Genomics 13:555CrossRefGoogle Scholar
  23. Veltri A, Lang C, Lien WH (2018) Concise review: Wnt signaling pathways in skin development and epidermal stem cells. Stem Cells 36(1):22–35CrossRefGoogle Scholar
  24. Wang P, Zhao Y, Fan R et al (2016) MicroRNA-21a-5p functions on the regulation of melanogenesis by targeting Sox5 in mouse skin melanocytes. Int J Mol Sci 17(7):E959CrossRefGoogle Scholar
  25. Wang S, Ge W, Luo Z, Guo Y, Jiao B, Qu L, Zhang Z, Wang X (2017) Integrated analysis of coding genes and non-coding RNAs during hair follicle cycle of cashmere goat (Capra hircus). BMC Genomics 18(1):767CrossRefGoogle Scholar
  26. Zhang J, Liu Y, Zhu Z, Yang S, Ji K, Hu S, Liu X, Yao J, Fan R, Dong C (2017) Role of microRNA508-3p in melanogenesis by targeting microphthalmia transcription factor in melanocytes of alpaca. Animal 11(2):236–243CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Veterinary MedicineJilin UniversityChangchunChina
  2. 2.College of Animal Science and TechnologyJilin Agricultural Science and Technology UniversityJilinChina
  3. 3.College of Animal Science and TechnologyJilin Agricultural UniversityChangchunChina

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