Differential expression of miR-let7a in hair follicle cycle of Liaoning cashmere goats and identification of its targets
In order to improve the production and quality of Chinese cashmere, the research of hair follicle development has aroused more and more attention; the regulation mechanism of miRNA in hair follicle development has become a hot spot. A survey of transcriptome profiling screened 10 hair follicle-related miRNAs that were differentially expressed, including miR-let7a. In this study, the expression of miR-let7a was lower in anagen of hair follicle of cashmere goats than that in catagen of hair follicle of cashmere goats (p < 0.01). Results were in accordance with transcriptome data. The expression patterns of miR-let7a target genes (IGF-1R, C-myc, and FGF5) were verified by qRT-PCR, which were consistent with the results of Western blot and showed a downward trend. The dual-luciferase reporter gene system was used to verify the correlation between the expression of miR-let7a and its target genes, and it showed that miR-let7a negatively correlates with C-myc and FGF5. Present study offers new information on miRNAs and their related target genes in the regulation of hair follicle development mechanism.
KeywordsLiaoning cashmere goats miR-let7a Hair follicle development Differential expression
This research was supported by the Cashmere Goat Breeding Center of Liaoning province.
This work was supported by the Jilin Province Natural Science Foundation (20170101156JC), the Special Funds for Scientific Research on Public Causes (201303119), the Special Foundation for Postdoctor of China Ministry of Education (No. 20100471261), and the grants from the National Natural Science Foundation of China (NSFC) (Nos. 30800807 and 31072097).
Compliance with ethical standards
This study was conducted in strict compliance with the recommendations of under the guidance of Jilin University Animal Care and Use Committee.
Conflict of interest
The authors declare that they have no conflict of interest.
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