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Genome sequence and comparative analysis of Bacillus cereus BC04, reveals genetic diversity and alterations for antimicrobial resistance

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Abstract

In this study, we delineated the genome sequence of a Bacillus cereus strain BC04 isolated from a stool sample in India. The draft genome is 5.1 Mb in size and consists of total 109 scaffolds, GC content is 35.2% with 5182 coding genes. The comparative analysis with other completely sequenced genomes highlights the unique presence of genomic islands, hemolysin, capsular synthetic protein, modifying enzymes accC7 and catA15, regulators of antibiotic resistance MarR and LysR with annotated functions related to virulence, stress response, and antimicrobial resistance. Overall, this study not only signifies the genetic diversity in gut isolate BC04 in particular, but also pinpoints the presence of unique genes possessed by B. cereus which can be pertinently exploited to design novel drugs and intervention strategies for the treatment of food borne diseases.

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Acknowledgements

We are profoundly thankful to Director CSIR-Institute of Microbial Technology, Chandigarh, for providing an excellent facility to carry out this work. VBS remains extremely grateful to the DST (No. SB/YS/LS-177/2014) for their excellent and timely grant-in-aid and the fellowship.

Funding

The funding received from CSIR (BSC0119H), DBT (BT/HRD/NBA/34/01/2012, BT/01/IYBA/2009), and DST (SB/YS/LS-177/2014) are highly acknowledged, however, had no role in study design, data collection, and analysis, decision to publish, or preparation of the manuscript.

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Authors and Affiliations

  1. Bacterial Signaling and Drug Resistance Laboratory, CSIR-Institute of Microbial Technology, Sector 39-A, Chandigarh, 160036, India

    Vijaya Bharathi Srinivasan, Mahavinod Angrasan, Neha Chandel & Govindan Rajamohan

Authors
  1. Vijaya Bharathi Srinivasan
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  2. Mahavinod Angrasan
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  3. Neha Chandel
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  4. Govindan Rajamohan
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Contributions

VBS and GR conceived the study, design experiments, performed experiments, executed the analysis and have drafted, revised and finalized the manuscript. VA and NC validated the disc susceptibility and MIC determination. Authors have read and approved the manuscript.

Corresponding author

Correspondence to Govindan Rajamohan.

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The authors declare that they have no conflict of interest.

Electronic supplementary material

ESM 1

(DOC 190 kb)

SFigure 1

The whole genome based phylogenetic tree using B. cereus genomes (complete and incomplete) from NCBI. The tree was created using iTOL (https://itol.embl.de). (GIF 114 kb)

High resolution image

(TIFF 2299 kb)

SFigure 2

The graph shows the percentage of proteins involved in different physiological functions as extracted from RAST annotation of B. cereus BC04 strain. (GIF 25.1 kb)

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(TIFF 592 kb)

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Srinivasan, V.B., Angrasan, M., Chandel, N. et al. Genome sequence and comparative analysis of Bacillus cereus BC04, reveals genetic diversity and alterations for antimicrobial resistance. Funct Integr Genomics 18, 477–487 (2018). https://doi.org/10.1007/s10142-018-0600-5

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